scholarly journals Improving CoQ10 productivity by strengthening glucose transmembrane of Rhodobacter sphaeroides

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yuying Yang ◽  
Lu Li ◽  
Haoyu Sun ◽  
Zhen Li ◽  
Zhengliang Qi ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Growth Inhibition ◽  
Rhodobacter Sphaeroides ◽  
Bacterial Growth ◽  
Rapid Growth ◽  
Biomass Yield ◽  
Phosphotransferase System ◽  
Maximum Biomass ◽  
Bacterial Growth Inhibition

Abstract Background Several Rhodobacter sphaeroides have been widely applied in commercial CoQ10 production, but they have poor glucose use. Strategies for enhancing glucose use have been widely exploited in R. sphaeroides. Nevertheless, little research has focused on the role of glucose transmembrane in the improvement of production. Results There are two potential glucose transmembrane pathways in R. sphaeroides ATCC 17023: the fructose specific-phosphotransferase system (PTSFru, fruAB) and non-PTS that relied on glucokinase (glk). fruAB mutation revealed two effects on bacterial growth: inhibition at the early cultivation phase (12–24 h) and promotion since 36 h. Glucose metabolism showed a corresponding change in characteristic vs. the growth. For ΔfruAΔfruB, maximum biomass (Biomax) was increased by 44.39% and the CoQ10 content was 27.08% more than that of the WT. glk mutation caused a significant decrease in growth and glucose metabolism. Over-expressing a galactose:H+ symporter (galP) in the ΔfruAΔfruB relieved the inhibition and enhanced the growth further. Finally, a mutant with rapid growth and high CoQ10 titer was constructed (ΔfruAΔfruB/tac::galPOP) using several glucose metabolism modifications and was verified by fermentation in 1 L fermenters. Conclusions The PTSFru mutation revealed two effects on bacterial growth: inhibition at the early cultivation phase and promotion later. Additionally, biomass yield to glucose (Yb/glc) and CoQ10 synthesis can be promoted using fruAB mutation, and glk plays a key role in glucose metabolism. Strengthening glucose transmembrane via non-PTS improves the productivity of CoQ10 fermentation.

scholarly journals Improving CoQ10 Productivity by Strengthening Glucose Transmembrane of Rhodobacter Sphaeroides

2021 ◽  
Author(s):  
Yuying Yang ◽  
Lu Li ◽  
Haoyu Sun ◽  
Zhen Li ◽  
Qi Zhengliang ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Growth Inhibition ◽  
Rhodobacter Sphaeroides ◽  
Bacterial Growth ◽  
Rapid Growth ◽  
Biomass Yield ◽  
Phosphotransferase System ◽  
Maximum Biomass ◽  
Bacterial Growth Inhibition

Abstract Background: Many Rhodobacter sphaeroides have been widely applied in commercial CoQ10 production, but they have poor glucose use. Strategies for enhancing glucose use have been widely exploited in R. sphaeroides. Nevertheless, little research has focused on the role of glucose transmembrane in the improvement of production.Results: There are two potential glucose transmembrane pathways in R. sphaeroides ATCC 17023: the fructose specific-phosphotransferase system (PTSFru, fruAB) and non-PTS that relied on glucokinase (glk). fruAB mutation revealed two effects on bacterial growth: inhibition at the early cultivation phase (12-24 h) and promotion since 36 h. Glucose metabolism showed a corresponding change in characteristic vs. the growth. For △fruA△fruB, maximum biomass (Biomax) was increased by 44.39 % and the CoQ10 content was 27.08 % more than that of the WT. glk mutation caused a significant decrease in growth and glucose metabolism. Overexpressing a galactose:H+ symporter (galP) in the △fruA△fruB relieved the inhibition and enhanced the growth further. Finally, a mutant with rapid growth and high CoQ10 yield was constructed (△fruA△fruB/tac::galPOP) using several glucose metabolism modifications and was verified by fermentation in a 10-L fermenter.Conclusions: The PTSFru mutation revealed two effects on bacterial growth: inhibition at the early cultivation phase and promotion later. Additionally, biomass yield to glucose (Ybio/glc) and CoQ10 synthesis can be promoted using fruAB mutation, and glk plays a key role in glucose metabolism. Strengthening glucose transmembrane via non-PTS improves the productivity of CoQ10 fermentation.

scholarly journals Reversal by Ribonucleosides of Bacterial Growth Inhibition Caused by Alcohol

1960 ◽  
Vol 235 (12) ◽  
pp. 3551-3555
Author(s):  
Edwin M. Lansford ◽  
Ira D. Hill ◽  
William Shive
Keyword(s):  
Growth Inhibition ◽  
Bacterial Growth ◽  
Bacterial Growth Inhibition

scholarly journals Natural Product Rottlerin Derivatives Targeting Quorum Sensing

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3745
Author(s):  
Dittu Suresh ◽  
Shekh Sabir ◽  
Tsz Tin Yu ◽  
Daniel Wenholz ◽  
Theerthankar Das ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Growth Inhibition ◽  
Natural Product ◽  
Bacterial Growth ◽  
Inhibitory Activity ◽  
Chalcone Derivatives ◽  
Synthetic Strategy ◽  
The Mannich Reaction ◽  
Bacterial Growth Inhibition

Rottlerin is a natural product consisting of chalcone and flavonoid scaffolds, both of which have previously shown quorum sensing (QS) inhibition in various bacteria. Therefore, the unique rottlerin scaffold highlights great potential in inhibiting the QS system of Pseudomonas aeruginosa. Rottlerin analogues were synthesised by modifications at its chalcone- and methylene-bridged acetophenone moieties. The synthesis of analogues was achieved using an established five-step synthetic strategy for chalcone derivatives and utilising the Mannich reaction at C6 of the chromene to construct morpholine analogues. Several pyranochromene chalcone derivatives were also generated using aldol conditions. All the synthetic rottlerin derivatives were screened for QS inhibition and growth inhibition against the related LasR QS system. The pyranochromene chalcone structures displayed high QS inhibitory activity with the most potent compounds, 8b and 8d, achieving QS inhibition of 49.4% and 40.6% and no effect on bacterial growth inhibition at 31 µM, respectively. Both compounds also displayed moderate biofilm inhibitory activity and reduced the production of pyocyanin.

scholarly journals Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by1H NMR

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Halyna Hrynash ◽  
Vinay Kumar Pilly ◽  
Alexandra Mankovskaia ◽  
Yaoyang Xiong ◽  
Getulio Nogueira Filho ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Inhibition ◽  
Bacterial Growth ◽  
Testing Machine ◽  
Control Group ◽  
Uv Spectrophotometry ◽  
Release Rates ◽  
H Nmr ◽  
Colony Forming Units ◽  
Bacterial Growth Inhibition

Objective.To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY;Vaccinium macrocarpon).Methods.Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture (Streptococcus mutans) for 24 h. Cell viability (n=3) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus (E) were tested on a universal testing machine (n=8). Compound release and chemical stability were evaluated by UV spectrophotometry and1H NMR (n=3). Data were analyzed by one-way ANOVA and Tukey’s test (α= 0.05).Results.Both compounds inhibited S. mutans growth, with CHX being most effective (P<0.05). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P<0.05). The 24 h compound release rates were ACY: 1.33 μg/mL and CHX: 1.92 μg/mL.1H NMR spectra suggests that both compounds remained stable after being released in water.Conclusion.The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.

scholarly journals Microbial Growth Inhibition by Alternating Electric Fields in Mice with Pseudomonas aeruginosa Lung Infection

2010 ◽  
Vol 54 (8) ◽  
pp. 3212-3218 ◽  
Author(s):  
Moshe Giladi ◽  
Yaara Porat ◽  
Alexandra Blatt ◽  
Esther Shmueli ◽  
Yoram Wasserman ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Growth Inhibition ◽  
Electric Fields ◽  
Bacterial Growth ◽  
Lung Infection ◽  
Alternating Electric Fields ◽  
Microbial Growth Inhibition ◽  
Bacterial Growth Inhibition

ABSTRACT High-frequency, low-intensity electric fields generated by insulated electrodes have previously been shown to inhibit bacterial growth in vitro. In the present study, we tested the effect of these antimicrobial fields (AMFields) on the development of lung infection caused by Pseudomonas aeruginosa in mice. We demonstrate that AMFields (10 MHz) significantly inhibit bacterial growth in vivo, both as a stand-alone treatment and in combination with ceftazidime. In addition, we show that peripheral (skin) heating of about 2°C can contribute to bacterial growth inhibition in the lungs of mice. We suggest that the combination of alternating electric fields, together with the heat produced during their application, may serve as a novel antibacterial treatment modality.

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