A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

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A case series of medically managed Candida Parapsilosis complex prosthetic valve endocarditis and literature review

10.21203/rs.3.rs-97836/v1 ◽

2020 ◽

Author(s):

Penghao Guo ◽

Yuting He ◽

Rui Fan ◽

Zhongwen Wu ◽

Yili Chen ◽

...

Keyword(s):

Prosthetic Valve ◽

Candida Parapsilosis ◽

Case Series ◽

Prosthetic Valve Endocarditis ◽

Sensu Stricto ◽

Therapeutic Strategies ◽

Case Presentation ◽

Candida Metapsilosis ◽

Candida Parapsilosis Complex ◽

Candida Orthopsilosis

Abstract Background: In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis and Candida Parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. Our paper is the first to report cases as Candida Parapsilosis complex PVE. Case presentation: A series of 4 cases of Candida Parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions: More attention should be paid to Candida Parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida Parapsilosis complex PVE

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Identification of Candida parapsilosis Species Complex and Lodderomyces elongisporus Isolates with MALDI-TOF MS

Türk Mikrobiyoloji Cemiyeti Dergisi ◽

10.5222/tmcd.2021.59389 ◽

2021 ◽

Author(s):

Engin Kaplan ◽

Ayşe Sultan Karakoyun ◽

Deniz Alkaya ◽

Nevzat Ünal ◽

Aylin Döğen ◽

...

Keyword(s):

Species Complex ◽

Candida Parapsilosis ◽

Antifungal Susceptibility ◽

Maldi Tof Ms ◽

Biochemical Methods ◽

Maldi Tof ◽

Candida Orthopsilosis ◽

Susceptibility Profiles ◽

Reference Strains ◽

Tof Ms

Objective: Candida parapsilosis species complex and Lodderomyces elongisporus may have differences in terms of their virulence, prevalence, and antifungal susceptibility profiles. These species are difficult to identify with biochemical methods. Therefore, there is a need for more efficient identification methods in terms of time, cost, and applicability. This study aims to evaluate the diagnostic performance of the MALDI-TOF MS method in discriminating between isolates belonging to the C. parapsilosis species complex and L. elongisporus. Method: In the current study, a total of 32 reference strains, including the C. parapsilosis (n=8), Candida orthopsilosis (n=7), Candida metapsilosis (n=6), and L. elongisporus (n=11) species were identified using the MALDI-TOF MS method. Results: The species names of 31 (93.7%) isolates belonging to the C. parapsilosis species complex and L.elongisporus were correctly identified. Twenty four isolates including eight (100%) C. parapsilosis, five (83%) C. metapsilosis, five (71%) C. orthopsilosis, and six (54%) L. elongisporus isolates were identified with score values ranging from 1.7 to 2.14. According to the secure identification reference score of ≥ 1.7, the sensitivity and specificity of the MALDI-TOF MS method were determined as 54.5–100% and 96.3–100%, respectively. Conclusion: Although the MALDI-TOF MS method has been shown to be effective in the rapid molecular phenotypic diagnosis of species that were difficult to discriminate using biochemical methods such as C. parapsilosis species complex and L. elongisporus, there is a clear need to optimize the method and develop a larger MS library for species-level identification within secure score ranges.

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Evaluation of capacity to detect ability to form biofilm in Candida parapsilosis sensu stricto strains by MALDI-TOF MS

Folia Microbiologica ◽

10.1007/s12223-016-0458-7 ◽

2016 ◽

Vol 61 (6) ◽

pp. 465-471 ◽

Cited By ~ 1

Author(s):

Katarína Mlynáriková ◽

Ondrej Šedo ◽

Filip Růžička ◽

Zbyněk Zdráhal ◽

Veronika Holá ◽

...

Keyword(s):

Candida Parapsilosis ◽

Sensu Stricto ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Combination antifungal therapy for treatment of Candida parapsilosis prosthetic valve endocarditis and utility of T2Candida Panel®: A case series

IDCases ◽

10.1016/j.idcr.2019.e00525 ◽

2019 ◽

Vol 15 ◽

pp. e00525 ◽

Cited By ~ 2

Author(s):

Tania Ahuja ◽

Karen Fong ◽

Eddie Louie

Keyword(s):

Antifungal Therapy ◽

Prosthetic Valve ◽

Candida Parapsilosis ◽

Case Series ◽

Prosthetic Valve Endocarditis

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Screening of strains of the Candida parapsilosis group of the BCCM/IHEM collection by MALDI-TOF MS

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2011.04.006 ◽

2011 ◽

Vol 70 (4) ◽

pp. 544-548 ◽

Cited By ~ 15

Author(s):

Marijke Hendrickx ◽

Jean-Sebastien Goffinet ◽

Danielle Swinne ◽

Monique Detandt

Keyword(s):

Candida Parapsilosis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Identification and typing of the Candida parapsilosis complex: MALDI-TOF MS vs. AFLP

Medical Mycology ◽

10.1093/mmy/myt009 ◽

2014 ◽

Vol 52 (2) ◽

pp. 123-130 ◽

Cited By ~ 28

Author(s):

E. D. Carolis ◽

L. A. M. Hensgens ◽

A. Vella ◽

B. Posteraro ◽

M. Sanguinetti ◽

...

Keyword(s):

Candida Parapsilosis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Candida Parapsilosis Complex ◽

Tof Ms

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High index of suspicion for brucellosis in a highly cosmopolitan city in southern China

10.21203/rs.2.10517/v3 ◽

2019 ◽

Author(s):

Hai-Yan Ye ◽

Fan-Fan Xing ◽

Jin Yang ◽

Simon Kam-Fai Lo ◽

Ricky Wing-Tong Lau ◽

...

Keyword(s):

Risk Factors ◽

Southern China ◽

Clinical Specimen ◽

Case Series ◽

Guangdong Province ◽

Human Brucellosis ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Chest Wall Abscess ◽

Tof Ms

Abstract Background: Brucellosis is one of the most widespread zoonosis in the world. In China, 90% of human brucellosis occurs in six northern agricultural provinces. However, there is a recent increase in the trend of human brucellosis in southern provinces with limited cases reported in the literature. Our study aims to describe the clinical features and epidemiology of brucellosis in a tertiary hospital in southern China. Methods: A retrospective case series of brucellosis was conducted between January 1, 2014 and October 31. 2018. Cases were identified based on positive Brucella serology by tube agglutination test, or positive culture from clinical specimen identified by Vitek 2 and MALDL-TOF MS. Clinical details of brucellosis including patients’ occupation, risk factors, and complications were analyzed. Clinical characteristics between patients from Guangdong and other provinces were also compared. Results: A total of 13 cases of laboratory-confirmed brucellosis were identified. 7 (53.8%) of the patients were male, 6 (46.2%) were female, with age ranging from 29 to 73 years old (median age: 51 years). 5 patients (38.5%) were from Guangdong province, while the remaining patients (61.5%) were from other provinces. The commonest risk factors of acquisition were consumption of undercooked meat and goat placenta. Patients from Guangdong province were found to be more likely to have prior placenta consumption. The commonest clinical presentations were fever, osteoarticular pain, urinary symptoms, splenomegaly, and lymphadenopathy. Spondylodiscitis/ peripheral joint arthritis (5 patients, 38.5%) was the most prevalent complication, while extra-osteoarticular complications including abdominal aortitis, hepatosplenic abscess, chest wall abscess, and epididymo-orchitis were observed in 4 other patients. Furthermore, it was demonstrated that MALDI-TOF MS is reliable in Brucella identification after additional of reference spectra with standard Brucella strain.Conclusions: Brucellosis, previously thought to be only found in northern China, is now increasingly seen in highly cosmopolitan part of southern China. MALDI-TOF MS in hospitals in China should include reference spectra with standard Brucella strain to aid bacterial identification in routine clinical practice. In addition to tuberculosis, typhoid fever and typhus, brucellosis should be considered in patients with fever of unknown origin in this locality.

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