Immune and Epstein-Barr virus gene expression in cerebrospinal fluid and peripheral blood mononuclear cells from patients with relapsing-remitting multiple sclerosis

Peripheral blood mononuclear cells from 10 patients with rheumatoid arthritis and 9 control subjects were cultured in vitro for 30 days with and without infection by Epstein-Barr virus. All cultures showed polyclonal stimulation of B cells as indicated by rising levels of IgM in the culture supernates, reaching maximal at 18-24 days, and with no quantitative or kinetic difference between the RA and control cells. IgM anti-IgG was also produced in both groups and maximally at 18-24 days, but in greater quantity by the RA lymphocytes. The anti-IgG made by the RA lymphocytes was more easily absorbed by solid phase IgG than was the anti-IgG made by the normal lymphocytes and thus was judged to be of higher affinity. RA lymphocytes uninfected with EBV had higher transformation scores than did the normal controls and developed spontaneously into permanent cell lines in six instances.

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An assessment of the frequency of lymphocytes with IL-4 intracellular expression and IL-4 concentration in the plasma of patients with chronic lymphocytic leukemia depending on the occurrence of Epstein-Barr virus DNA in peripheral blood mononuclear cells

Postępy Nauk Medycznych ◽

10.5604/08606196.1157913 ◽

2015 ◽

Vol 28 (6) ◽

pp. 378-383

Author(s):

Ewelina Grywalska ◽

Jacek Roliński ◽

Agnieszka Grafka ◽

Maciej Maj ◽

Michał Mielnik ◽

...

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood Mononuclear Cells ◽

Epstein Barr Virus ◽

Mononuclear Cells ◽

Lymphocytic Leukemia ◽

Peripheral Blood Mononuclear ◽

Barr Virus ◽

Blood Mononuclear Cells ◽

Intracellular Expression ◽

Epstein Barr

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No significant effect of orally administered chemokine receptor 1 antagonist on intercellular adhesion molecule-3 expression in relapsing—remitting multiple sclerosis patients

Multiple Sclerosis Journal ◽

10.1177/1352458509358188 ◽

2010 ◽

Vol 16 (3) ◽

pp. 366-369 ◽

Cited By ~ 5

Author(s):

R. Reuβ ◽

V. Schreiber ◽

A. Klein ◽

C. Infante-Duarte ◽

M. Filippi ◽

...

Keyword(s):

Multiple Sclerosis ◽

Peripheral Blood Mononuclear Cells ◽

Chemokine Receptor ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Intercellular Adhesion ◽

Peripheral Blood Mononuclear ◽

Relapsing Remitting ◽

Blood Mononuclear Cells ◽

Relapsing Remitting Multiple Sclerosis

We investigated the expression of intercellular adhesion molecules ICAM-1 and ICAM-3 on peripheral blood mononuclear cells in a subgroup of 34 patients with relapsing-remitting multiple sclerosis who were treated orally with the chemokine receptor 1 antagonist BX 471 in a 16-week, randomised, double-blind, placebo-controlled phase II study. ICAM-1 and ICAM-3 expression was measured by flow cytometry at different time points during and after therapy and compared using multivariate analysis of variance and non-parametric Mann Whitney test. ICAM-3 expression on CD14 + peripheral blood mononuclear cells was increased in the verum group under therapy, but did not differ significantly between the verum and placebo groups. Most likely, this trend represents a small epiphenomenon only mediated by receptor cross-talk and feedback mechanisms.

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