scholarly journals Real-time reverse transcription PCR-based sequencing-independent pathotyping of Eurasian avian influenza A viruses of subtype H7

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Annika Graaf ◽  
Martin Beer ◽  
Timm Harder
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
Influenza A Viruses ◽  
Reverse Transcription Pcr

scholarly journals Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR

2006 ◽  
Vol 45 (2) ◽  
pp. 600-603 ◽  
Author(s):  
B. Hoffmann ◽  
T. Harder ◽  
E. Starick ◽  
K. Depner ◽  
O. Werner ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
H5n1 Virus ◽  
Reverse Transcription Pcr ◽  
Highly Sensitive

scholarly journals Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China

2013 ◽  
Vol 18 (16) ◽  
Author(s):  
V M Corman ◽  
M Eickmann ◽  
O Landt ◽  
T Bleicker ◽  
S Brünink ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
Specific Detection ◽  
Validation Data ◽  
Reverse Transcription Pcr ◽  
Polymerase Chain ◽  
Pcr Assays ◽  
Positive Controls

In response to a recent outbreak in China, detection assays for a novel avian influenza A(H7N9) virus need to be implemented in a large number of public health laboratories. Here we present real-time reverse-transcription polymerase chain reaction (RT-PCR) assays for specific detection of this virus, along with clinical validation data and biologically-safe positive controls.

scholarly journals Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

2021 ◽  
pp. 104063872199481
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Infectious Dose ◽  
The Matrix ◽  
Taqman Minor Groove Binder

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

Detection of avian influenza A/H7N9/2013 virus by real-time reverse transcription-polymerase chain reaction

2014 ◽  
Vol 206 ◽  
pp. 140-143 ◽  
Author(s):  
Xiaoping Kang ◽  
Weili Wu ◽  
Chuntao Zhang ◽  
Licheng Liu ◽  
Huahua Feng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document