Down-regulated miR-22 as predictive biomarkers for prognosis of epithelial ovarian cancer

IntroductionEpithelial ovarian cancer (EOC) is the deadliest gynecologic malignancy worldwide. Reliable predictive biomarkers are urgently needed to estimate the risk of relapse and to improve treatment management. Soluble immune-checkpoints in EOC are promising molecules serving as prognostic biomarkers accessible via liquid biopsy. We thus, aimed at elucidating the role of sB7-H4 in EOC.Material and MethodsWe analyzed soluble serum B7-H4 (sB7-H4) using ELISA and circulating tumor cells (CTCs) in blood applying the AdnaTest OvarianCancer in 85 patients suffering from advanced EOC. Findings were correlated with clinical parameters as well as survival data.ResultssB7-H4 was detectable in 14.1% patients, CTCs in 32.9% patients and simultaneous presence of CTCs and sB7-H4 was found in 7% patients, respectively. Although no association between sB7-H4 and CTC could be documented, each of them served as independent predictive factors for overall survival (OS).ConclusionsB7-H4 and CTCs are independent prognostic biomarkers for impaired survival in EOC. As they are easily accessible via liquid biopsy, they may be of potential benefit for the prediction of therapy response and survival for EOC patients.

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Measurements of adiposity as predictive biomarkers for response to anti-angiogenic treatment in epithelial ovarian cancer: An NRG/GOG ancillary data analysis of GOG 218

Gynecologic Oncology ◽

10.1016/j.ygyno.2017.03.086 ◽

2017 ◽

Vol 145 ◽

pp. 32-33

Author(s):

K.N. Slaughter ◽

M.F. Brady ◽

T. Thai ◽

Y. Wang ◽

B. Zheng ◽

...

Keyword(s):

Ovarian Cancer ◽

Data Analysis ◽

Epithelial Ovarian Cancer ◽

Predictive Biomarkers ◽

Ancillary Data

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Abstract B04: Immune modeling analysis identifies ICOS and CTLA-4 as predictive biomarkers in serous epithelial ovarian cancer

10.1158/1557-3265.ovca19-b04 ◽

2020 ◽

Author(s):

Nicole James ◽

Matthew Oliver ◽

Joyce Ou ◽

Jenna Emerson ◽

Katherine Miller ◽

...

Keyword(s):

Ovarian Cancer ◽

Epithelial Ovarian Cancer ◽

Predictive Biomarkers ◽

Modeling Analysis ◽

Serous Epithelial Ovarian Cancer

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Measurements of adiposity as predictive biomarkers for response to first-line bevacizumab-based chemotherapy in epithelial ovarian cancer (EOC)

Gynecologic Oncology ◽

10.1016/j.ygyno.2014.03.451 ◽

2014 ◽

Vol 133 ◽

pp. 171

Author(s):

K.N. Slaughter ◽

T. Thai ◽

S. Penaroza ◽

D.M. Benbrook ◽

E. Thavathiro ◽

...

Keyword(s):

Ovarian Cancer ◽

Epithelial Ovarian Cancer ◽

Predictive Biomarkers ◽

First Line

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Calcineurin Gamma Catalytic Subunit PPP3CC Inhibition by miR-200c-3p Affects Apoptosis in Epithelial Ovarian Cancer

Genes ◽

10.3390/genes12091400 ◽

2021 ◽

Vol 12 (9) ◽

pp. 1400

Author(s):

Eleni Anastasiadou ◽

Elena Messina ◽

Tiziana Sanavia ◽

Vittorio Labruna ◽

Simona Ceccarelli ◽

...

Keyword(s):

Ovarian Cancer ◽

Epithelial Ovarian Cancer ◽

Mrna Expression ◽

Target Genes ◽

Cancer Cell Line ◽

Predictive Biomarkers ◽

Catalytic Subunit ◽

The Cancer Genome Atlas ◽

Apoptosis Pathway

Epithelial ovarian cancer (EOC) outpaces all the other forms of the female reproductive system malignancies. MicroRNAs have emerged as promising predictive biomarkers to therapeutic treatments as their expression might characterize the tumor stage or grade. In EOC, miR-200c is considered a master regulator of oncogenes or tumor suppressors. To investigate novel miR-200c-3p target genes involved in EOC tumorigenesis, we evaluated the association between this miRNA and the mRNA expression of several potential target genes by RNA-seq data of both 46 EOC cell lines from Cancer Cell line Encyclopedia (CCLE) and 456 EOC patient bio-specimens from The Cancer Genome Atlas (TCGA). Both analyses showed a significant anticorrelation between miR-200c-3p and the protein phosphatase 3 catalytic subunit γ of calcineurin (PPP3CC) levels involved in the apoptosis pathway. Quantitative mRNA expression analysis in patient biopsies confirmed the inverse correlation between miR-200c-3p and PPP3CC levels. In vitro regulation of PPP3CC expression through miR-200c-3p and RNA interference technology led to a concomitant modulation of BCL2- and p-AKT-related pathways, suggesting the tumor suppressive role of PPP3CC in EOC. Our results suggest that inhibition of high expression of miR-200c-3p in EOC might lead to overexpression of the tumor suppressor PPP3CC and subsequent induction of apoptosis in EOC patients.

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