Correction to: microRNA-193a stimulates pancreatic cancer cell repopulation and metastasis through modulating TGF-β2/TGF-βRIII signalings

Abstract Background : Pancreatic cancer is one of most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is caused by the residual surviving cells repopulation stimulated by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation. Methods: Next generation sequencing (NGS) and bioinformatics were performed to analyze and annotate the expression and function of exosome-derived circRNAs in pancreatic cells during radiation. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing results. Results: In this study, 3,580 circRNAs were annotated in literatures and circBase among 12,572 identified circRNAs. There were 196 filtered differentially expressed circRNAs (the up-regulation and down-regulation respectively is 182 and 14, fold change >2, p-value <0.05). Regulation of metabolic process and lysine degradation were the main biological processes and pathway enrichment according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Conclusions: The hsa_circ_0002130-hsa_miR_4482-3p-NBN interaction network suggested potential miRNA sponge and target mRNA. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell repopulation upon irradiation.

Download Full-text

Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation

10.21203/rs.2.13331/v5 ◽

2020 ◽

Author(s):

Yi-yun Chen ◽

Ming-jie Jiang ◽

Zhi-long Chen ◽

Ling Tian

Keyword(s):

Pancreatic Cancer ◽

Cancer Cell ◽

Molecular Mechanisms ◽

Pancreatic Cancer Cell ◽

Pcr Analysis ◽

Circular Rnas ◽

P Value ◽

Pancreatic Cancer Cells ◽

Dying Cells ◽

Cell Repopulation

Abstract Background: Pancreatic cancer is one of the most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is caused by the residual surviving cells repopulation stimulated by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation.Methods: Next generation sequencing (NGS) and bioinformatics were performed to analyze and annotate the expression and function of exosome-derived circRNAs in pancreatic cancer cells after radiation. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing results.Results: In this study, 3,580 circRNAs were annotated in literatures and circBase among 12,572 identified circRNAs. There were 196 filtered differentially expressed circRNAs (the up-regulation and down-regulation respectively is 182 and 14, fold change >2, p-value <0.05). Regulation of metabolic process and lysine degradation were the main enriched biological processes and pathway according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Conclusions: The hsa_circ_0002130-hsa_miR_4482-3p-NBN interaction network suggested potential sponging miRNA and target mRNA. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell repopulation upon irradiation.

Download Full-text

Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation

10.21203/rs.2.13331/v4 ◽

2020 ◽

Author(s):

Yi-yun Chen ◽

Ming-jie Jiang ◽

Zhi-long Chen ◽

Ling Tian

Keyword(s):

Pancreatic Cancer ◽

Cancer Cell ◽

Molecular Mechanisms ◽

Pancreatic Cancer Cell ◽

Pcr Analysis ◽

Circular Rnas ◽

P Value ◽

Pancreatic Cancer Cells ◽

Dying Cells ◽

Cell Repopulation

Abstract Background: Pancreatic cancer is one of the most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is caused by the residual surviving cells repopulation stimulated by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation. Methods: Next generation sequencing (NGS) and bioinformatics were performed to analyze and annotate the expression and function of exosome-derived circRNAs in pancreatic cancer cells after radiation. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing results. Results: In this study, 3,580 circRNAs were annotated in literatures and circBase among 12,572 identified circRNAs. There were 196 filtered differentially expressed circRNAs (the up-regulation and down-regulation respectively is 182 and 14, fold change >2, p-value <0.05). Regulation of metabolic process and lysine degradation were the main enriched biological processes and pathway according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Conclusions: The hsa_circ_0002130-hsa_miR_4482-3p-NBN interaction network suggested potential sponging miRNA and target mRNA. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell repopulation upon irradiation.

Download Full-text

microRNA-193a stimulates pancreatic cancer cell repopulation and metastasis through modulating TGF-β2/TGF-βRIII signalings

Journal of Experimental & Clinical Cancer Research ◽

10.1186/s13046-018-0697-3 ◽

2018 ◽

Vol 37 (1) ◽

Cited By ~ 12

Author(s):

Chi Fang ◽

Chen-yun Dai ◽

Zhu Mei ◽

Ming-jie Jiang ◽

Dian-na Gu ◽

...

Keyword(s):

Pancreatic Cancer ◽

Cancer Cell ◽

Pancreatic Cancer Cell ◽

Cell Repopulation

Download Full-text

Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation

10.21203/rs.2.13331/v2 ◽

2019 ◽

Author(s):

Yi-yun Chen ◽

Ming-jie Jiang ◽

Zhi-long Chen ◽

Ling Tian

Keyword(s):

Pancreatic Cancer ◽

Cancer Cell ◽

Molecular Mechanisms ◽

Pancreatic Cancer Cell ◽

Malignant Tumors ◽

Pcr Analysis ◽

Circular Rnas ◽

P Value ◽

Dying Cells ◽

Cell Repopulation

Abstract Background : Pancreatic cancer is one of most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is the cause of the residual surviving cells repopulation by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation. Methods: Next generation sequencing (NGS) and bioinformatics were performed to analysis and annotate the expressions and functions of exosome-derived circRNAs in pancreatic cells during radiation. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing. Results: In this study, 3580 circRNAs were annotated in literatures and circBase among 12572 identified circRNAs. And there were 196 filtered differentially expressed circRNAs (the up-regulation and down-regulation respectively is 182 and 14, fold change >2, p-value <0.05). Regulation of metabolic process and lysine degradation were the main biological processes and pathway enrichment according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Conclusions: The hsa_circ_0002130-hsa_miR_4482-3p-NBN interaction network suggested potential miRNA sponge and target mRNA. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell repopulation upon irradiation.

Download Full-text

Analysis of exosomal circRNAs upon irradiation in pancreatic cancer cell repopulation

10.21203/rs.2.13331/v1 ◽

2019 ◽

Author(s):

Yi-yun Chen ◽

Ming-jie Jiang ◽

Zhi-long Chen ◽

Ling Tian

Keyword(s):

Pancreatic Cancer ◽

Cancer Cell ◽

Molecular Mechanisms ◽

Pancreatic Cancer Cell ◽

Expression Profiles ◽

Pcr Analysis ◽

Circular Rnas ◽

P Value ◽

Dying Cells ◽

Cell Repopulation

Abstract Background Pancreatic cancer is one of most malignant tumors. However, radiotherapy can lead to tumor recurrence, which is the cause of the residual surviving cells repopulation by some molecular released from dying cells. Exosomes may mediate cell-cell communication and transfer kinds of signals from the dying cells to the surviving cells for stimulating tumor repopulation. Circular RNAs (circRNAs) may be one vital kind of exosomal cargos involving in modulating cancer cell repopulation. Methods Next generation sequencing (NGS) and bioinformatics were performed to analysis and annotate the expression profiles and functions of exosome-derived circRNAs in pancreatic cells during radiation. 4 circRNAs were chosen for qRT-PCR analysis to validate the sequencing. Results A total of 12572 circRNAs were identified, and 3580 circRNAs were annotated in literatures and circBase. There were 196 filtered differentially expressed circRNAs (182 for up-regulated and 14 for down-regulated, fold change >2, p-value <0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that regulation of metabolic process and lysine degradation were the main biological processes and pathway enrichment. Conclusions The hsa_circ_002130-hsa_miR_4482-3p-NBN interaction network suggested potential miRNA sponge and target mRNA. Our results provided potential functions of circRNAs to explore molecular mechanisms and therapeutic targets in pancreatic cancer cell repopulation upon irradiation.

Download Full-text