scholarly journals Functional characterization of the upstream components of the Hog1-like kinase cascade in hyperosmotic and carbon sensing in Trichoderma reesei

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhixing Wang ◽  
Ning An ◽  
Wenqiang Xu ◽  
Weixin Zhang ◽  
Xiangfeng Meng ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Functional Characterization ◽  
Kinase Cascade

scholarly journals Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210548 ◽  
Author(s):  
Jun He ◽  
Feng Tang ◽  
Daiwen Chen ◽  
Bing Yu ◽  
Yuheng Luo ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Functional Characterization ◽  
Thermostable Xylanase

Structural and functional characterization of the glutathione peroxidase-like thioredoxin peroxidase from the fungus Trichoderma reesei

2021 ◽  
Vol 167 ◽  
pp. 93-100
Author(s):  
Patricia P. Adriani ◽  
Fernanda C.R. de Paiva ◽  
Gabriel S. de Oliveira ◽  
Amanda C. Leite ◽  
Adriana S. Sanches ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Trichoderma Reesei ◽  
Functional Characterization ◽  
Thioredoxin Peroxidase

scholarly journals Identification and functional characterization of novel xylose transporters from the cell factories Aspergillus niger and Trichoderma reesei

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Jasper Sloothaak ◽  
Juan Antonio Tamayo-Ramos ◽  
Dorett I. Odoni ◽  
Thanaporn Laothanachareon ◽  
Christian Derntl ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Trichoderma Reesei ◽  
Functional Characterization ◽  
Cell Factories

scholarly journals Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Sami Havukainen ◽  
Jonai Pujol-Giménez ◽  
Mari Valkonen ◽  
Matthias A. Hediger ◽  
Christopher P. Landowski
Keyword(s):  
Trichoderma Reesei ◽  
Soluble Sugars ◽  
Functional Characterization ◽  
Value Added ◽  
High Specificity ◽  
Biofuel Production ◽  
Primary Metabolism ◽  
High Affinity ◽  
Xylose Transporter

Abstract Background Lignocellulose biomass has been investigated as a feedstock for second generation biofuels and other value-added products. Some of the processes for biofuel production utilize cellulases and hemicellulases to convert the lignocellulosic biomass into a range of soluble sugars before fermentation with microorganisms such as yeast Saccharomyces cerevisiae. One of these sugars is l-arabinose, which cannot be utilized naturally by yeast. The first step in l-arabinose catabolism is its transport into the cells, and yeast lacks a specific transporter, which could perform this task. Results We identified Trire2_104072 of Trichoderma reesei as a potential l-arabinose transporter based on its expression profile. This transporter was described already in 2007 as d-xylose transporter XLT1. Electrophysiology experiments with Xenopus laevis oocytes and heterologous expression in yeast revealed that Trire2_104072 is a high-affinity l-arabinose symporter with a Km value in the range of $$\sim$$ ∼  0.1–0.2 mM. It can also transport d-xylose but with low affinity (Km$$\sim$$ ∼  9 mM). In yeast, l-arabinose transport was inhibited slightly by d-xylose but not by d-glucose in an assay with fivefold excess of the inhibiting sugar. Comparison with known l-arabinose transporters revealed that the expression of Trire2_104072 enabled yeast to uptake l-arabinose at the highest rate in conditions with low extracellular l-arabinose concentration. Despite the high specificity of Trire2_104072 for l-arabinose, the growth of its T. reesei deletion mutant was only affected at low l-arabinose concentrations. Conclusions Due to its high affinity for l-arabinose and low inhibition by d-glucose or d-xylose, Trire2_104072 could serve as a good candidate for improving the existing pentose-utilizing yeast strains. The discovery of a highly specific l-arabinose transporter also adds to our knowledge of the primary metabolism of T. reesei. The phenotype of the deletion strain suggests the involvement of other transporters in l-arabinose transport in this species.

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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