scholarly journals Isolation and identification of Xenorhabdus and Photorhabdus bacteria associated with entomopathogenic nematodes and their larvicidal activity against Aedes aegypti

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Chamaiporn Fukruksa ◽  
Thatcha Yimthin ◽  
Manawat Suwannaroj ◽  
Paramaporn Muangpat ◽  
Sarunporn Tandhavanant ◽  
...  
PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195681 ◽  
Author(s):  
Temsiri Yooyangket ◽  
Paramaporn Muangpat ◽  
Raxsina Polseela ◽  
Sarunporn Tandhavanant ◽  
Aunchalee Thanwisai ◽  
...  

Author(s):  
Regina Geris ◽  
Ionizete Garcia da Silva ◽  
Heloísa Helena Garcia da Silva ◽  
Andersson Barison ◽  
Edson Rodrigues-Filho ◽  
...  

The objective of this study was to evaluate the larvicidal activity of diterpenoids obtained from the oil-resin of Copaifera reticulata against Aedes aegypti larvae, the principal vector of dengue and urban yellow fever. Four diterpenes were obtained from oil-resin extraction with organic solvents and subsequent chromatographic and spectroscopic procedures allowed to isolation and identification of these compounds as 3-b-acetoxylabdan-8(17)-13-dien-15-oic acid (1), alepterolic acid (2), 3-b-hidroxylabdan-8(17)-en-15-oic acid (3), and ent-agatic acid (4). Each compound was previously dissolved in dimethylsulphoxide, and distilled water was added to obtain the desired concentrations. Twenty larvae of third instars were placed into plastic beckers, containing the solution test (25 mL), in a five repetitions scheme, and their mortality, indicated by torpor and darkening of the cephalic capsule, was recorded after 48h. Probit analyses were used to determine lethal concentrations (LC50 and LC90) and their respective 95% confidence intervals. This study showed that only diterpenoids 1 and 2 exhibited larvicidal properties with LC50 of 0.8 ppm and 87.3 ppm, respectively, revealing the former as the most toxic compound against third instars of Ae. aegypti. Therefore, this compound seems to be an interesting source for new metabolite to be exploited.


2018 ◽  
pp. 362-374
Author(s):  
Harlyson L. Carvalho ◽  
Andréia L. de Amorim ◽  
Inana F. Araújo ◽  
Bianca L. B. Marino ◽  
David E. Q. Jimenez ◽  
...  

Author(s):  
A. M. S. RODRIGUES ◽  
J. E. DE PAULA ◽  
N. DEGALLIER ◽  
J. F. MOLEZ ◽  
L. S. ESPÍNDOLA

2004 ◽  
Vol 99 (5) ◽  
pp. 541-544 ◽  
Author(s):  
Eveline Solon Barreira Cavalcanti ◽  
Selene Maia de Morais ◽  
Michele Ashley A Lima ◽  
Eddie William Pinho Santana

2018 ◽  
Vol 16 (1) ◽  
pp. 324-332 ◽  
Author(s):  
Nurhayat Tabanca ◽  
Zulfiqar Ali ◽  
Ulrich R. Bernier ◽  
Nancy Epsky ◽  
Ayse Nalbantsoy ◽  
...  

AbstractAn ethanol extract fromVeratrum lobelianumBernh. rhizomes showed larvicidal activity with LC50values of 11.79 ppm and 89.9 ppm against 1stand 4thinstar larvae, respectively, at 24 h post-treatment. The extract also showed proportion not biting (PNB) value of 0.76 at 100 μg/cm2against females ofAe.aegypti. Systematic bioassay-guided fractionation ofV.lobelianumextract resulted in the isolation of five compounds that were identified as ethyl palmitate (1), ethyl linoleate (2), β-sitosterol (3), resveratrol (4) and oxyresveratrol (5) by GC-MS,1H-NMR, and13C-NMR techniques, comparison with literature data, and confirmation with authentic compounds. Compound2exhibited larvicidal activity with an LC50value of 24.1 (22.0-26.2) ppm whereas1was inactive. β-Sitosterol (3) displayed the highest larvicidal activity with LC50= 1.7 (1.3-12.3) ppm and LC90= 5.1 (3.4-13.8) ppm. Compounds4and5had larvicidal activity with LC50values of 18.5 (15.3-23.3) and 22.6 (19.0-26.8) ppm, respectively, and had an equivalent PNB values of 0.75 at 25 nmol/cm2. In addition,4and5were explored for their human-based repellency againstAe.aegypti, attractiveness against male medfliesCeratitis capitata, and also evaluated against series of human carcinoma cells (A549, HEK293, HeLa, SH-SY5Y); however, no significant activity was found.


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