scholarly journals Investigating the molecular basis of multiple insecticide resistance in a major malaria vector Anopheles funestus (sensu stricto) from Akaka-Remo, Ogun State, Nigeria

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Seun M. Atoyebi ◽  
Genevieve M. Tchigossou ◽  
Romaric Akoton ◽  
Jacob M. Riveron ◽  
Helen Irving ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Malaria Vector ◽  
Molecular Basis ◽  
Anopheles Funestus ◽  
Sensu Stricto ◽  
Ogun State ◽  
Major Malaria Vector

scholarly journals Exploring the Mechanisms of Multiple Insecticide Resistance in a Highly Plasmodium-Infected Malaria Vector Anopheles funestus Sensu Stricto from Sahel of Northern Nigeria

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 454
Author(s):  
Sulaiman S. Ibrahim ◽  
Muhammad M. Mukhtar ◽  
Helen Irving ◽  
Jacob M. Riveron ◽  
Amen N. Fadel ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Malaria Vector ◽  
Anopheles Funestus ◽  
Indoor Residual Spraying ◽  
Sensu Stricto ◽  
Bed Nets ◽  
Metabolic Resistance ◽  
Biting Rate ◽  
Insecticide Treated Bed Nets ◽  
Sahel Region

The Nigerian Government is scaling up the distribution of insecticide-treated bed nets for malaria control, but the lack of surveillance data, especially in the Sudan/Sahel region of the country, may hinder targeting priority populations. Here, the vectorial role and insecticide resistance profile of a population of a major malaria vector Anopheles funestus sensu stricto from Sahel of Nigeria was characterised. An. funestus s.s. was the only vector found, with a high human blood index (100%) and a biting rate of 5.3/person/night. High Plasmodium falciparum infection was discovered (sporozoite rate = 54.55%). The population is resistant to permethrin (mortality = 48.30%, LT50 = 65.76 min), deltamethrin, DDT (dichlorodiphenyltrichloroethane) and bendiocarb, with mortalities of 29.44%, 56.34% and 54.05%, respectively. Cone-bioassays established loss of efficacy of the pyrethroid-only long-lasting insecticidal nets (LLINs); but 100% recovery of susceptibility was obtained for piperonylbutoxide (PBO)-containing PermaNet®3.0. Synergist bioassays with PBO and diethyl maleate recovered susceptibility, implicating CYP450s (permethrin mortality = 78.73%, χ2 = 22.33, P < 0.0001) and GSTs (DDT mortality = 81.44%, χ2 = 19.12, P < 0.0001). A high frequency of 119F GSTe2 mutation (0.84) was observed (OR = 16, χ2 = 3.40, P = 0.05), suggesting the preeminent role of metabolic resistance. These findings highlight challenges associated with deployment of LLINs and indoor residual spraying (IRS) in Nigeria.

scholarly journals Multiple insecticide resistance in the major malaria vector Anopheles funestus in southern Ghana: implications for malaria control

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Jacob M. Riveron ◽  
Michael Osae ◽  
Alexander Egyir-Yawson ◽  
Helen Irving ◽  
Sulaiman S. Ibrahim ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Malaria Vector ◽  
Malaria Control ◽  
Anopheles Funestus ◽  
Southern Ghana ◽  
Major Malaria Vector

scholarly journals Evidence of a multiple insecticide resistance in the malaria vector Anopheles funestus in South West Nigeria

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Rousseau J. Djouaka ◽  
Seun M. Atoyebi ◽  
Genevieve M. Tchigossou ◽  
Jacob M. Riveron ◽  
Helen Irving ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Malaria Vector ◽  
Anopheles Funestus ◽  
South West

scholarly journals Cuticle thickening associated with pyrethroid resistance in the major malaria vector Anopheles funestus

2010 ◽  
Vol 3 (1) ◽  
pp. 67 ◽  
Author(s):  
O R Wood ◽  
S Hanrahan ◽  
M Coetzee ◽  
L L Koekemoer ◽  
B D Brooke
Keyword(s):  
Malaria Vector ◽  
Pyrethroid Resistance ◽  
Anopheles Funestus ◽  
Major Malaria Vector

scholarly journals Widespread Pyrethroid and DDT Resistance in the Major Malaria Vector Anopheles funestus in East Africa Is Driven by Metabolic Resistance Mechanisms

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e110058 ◽  
Author(s):  
Charles Mulamba ◽  
Jacob M. Riveron ◽  
Sulaiman S. Ibrahim ◽  
Helen Irving ◽  
Kayla G. Barnes ◽  
...  
Keyword(s):  
East Africa ◽  
Malaria Vector ◽  
Anopheles Funestus ◽  
Resistance Mechanisms ◽  
Metabolic Resistance ◽  
Ddt Resistance ◽  
Major Malaria Vector

scholarly journals Integration of whole genome sequencing and transcriptomics reveals a complex picture of insecticide resistance in the major malaria vector Anopheles coluzzii

2021 ◽  
Author(s):  
Victoria A Ingham ◽  
Jacob A Tennessen ◽  
Eric R Lucas ◽  
Sara Elg ◽  
Henrietta Carrington-Yates ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Malaria Vector ◽  
Anopheles Coluzzii ◽  
Whole Genome ◽  
Microbial Composition ◽  
Expression Of Genes ◽  
Oxidative Phosphorylation Pathway ◽  
Major Malaria Vector

Insecticide resistance is a major threat to gains in malaria control, which have been stalling and potentially reversing since 2015. Studies into the causal mechanisms of insecticide resistance are painting an increasingly complicated picture, underlining the need to design and implement targeted studies on this phenotype. In this study, we compare three populations of the major malaria vector An. coluzzii: a susceptible and two resistant colonies with the same genetic background. The original colonised resistant population rapidly lost resistance over a 6-month period, a subset of this population was reselected with pyrethroids a third population of this colony that did not lose resistance was also available. The original resistant, susceptible and re-selected colonies were subject to RNAseq and whole genome sequencing, which identified a number of changes across the transcriptome and genome linked with resistance. Firstly, an increase in the expression of genes within the oxidative phosphorylation pathway were seen in both resistant populations compared to the susceptible control; this translated phenotypically through an increased respiratory rate, indicating that elevated metabolism is linked directly with resistance. Genome sequencing highlighted several blocks clearly associated with resistance, including the 2Rb inversion. Finally, changes in the microbiome profile were seen, indicating that the microbial composition may play a role in the resistance phenotype. Taken together, this study reveals a highly complicated phenotype in which multiple transcriptomic, genomic and microbiome changes combine to result in insecticide resistance.

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