Microarray analysis of ductal carcinoma in situ samples obtained by puncture from surgical resection specimens

Abstract Objective The incidence of ductal carcinoma in situ (DCIS) is increasing due to more widespread mammographic screening. DCIS, the earliest form of breast cancer, is non-invasive at the time of detection. If DCIS tissues are left undetected or untreated, it can spread to the surrounding breast tissue. Thus, surgical resection is the standard treatment. Understanding the mechanism underlying the non-invasive property of DCIS could lead to more appropriate medical treatments, including nonsurgical options. Data description We conducted a microarray-based genome-wide transcriptome analysis using DCIS specimens obtained by puncture from surgical specimens immediately after surgery.

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Readmissions and complications in breast ductal carcinoma in situ: A retrospective study comparing screen- and non-screen-detected patients

Women s Health ◽

10.1177/1745506520965899 ◽

2020 ◽

Vol 16 ◽

pp. 174550652096589

Author(s):

Julieta Politi ◽

María Sala ◽

Laia Domingo ◽

María Vernet-Tomas ◽

Marta Román ◽

...

Keyword(s):

Breast Cancer ◽

Confidence Interval ◽

Ductal Carcinoma In Situ ◽

Odds Ratio ◽

Carcinoma In Situ ◽

Ductal Carcinoma ◽

Screening Program ◽

Population Based ◽

Mammographic Screening

Objective: Population-wide mammographic screening programs aim to reduce breast cancer mortality. However, a broad view of the harms and benefits of these programs is necessary to favor informed decisions, especially in the earliest stages of the disease. Here, we compare the outcomes of patients diagnosed with breast ductal carcinoma in situ in participants and non-participants of a population-based mammographic screening program. Methods: A retrospective cohort study of all patients diagnosed with breast ductal carcinoma in situ between 2000 and 2010 within a single hospital. A total of 211 patients were included, and the median follow-up was 8.4 years. The effect of detection mode (screen-detected and non-screen-detected) on breast cancer recurrences, readmissions, and complications was evaluated through multivariate logistic regression analysis. Results: In the majority of women, breast ductal carcinoma in situ was screen-detected (63.5%). Screen-detected breast ductal carcinoma in situ was smaller in size compared to those non-screen-detected (57.53% < 20 mm versus 78.03%, p = 0.002). Overall, breast-conserving surgery was the most frequent surgery (86.26%); however, mastectomy was higher in non-screen-detected breast ductal carcinoma in situ (20.78% versus 9.7%, p = 0.024). Readmissions for mastectomy were more frequent in non-screen-detected breast ductal carcinoma in situ. Psychological complications, such as fatigue, anxiety, and depression, had a prevalence of 15% within our cohort. Risk of readmissions and complications was higher within the non-screen-detected group, as evidenced by an odds ratio = 6.25 (95% confidence interval = 1.95–19.99) for readmissions and an odds ratio = 2.41 (95% confidence interval = 1.95–4.86) for complications. Conclusions: Our findings indicate that women with breast ductal carcinoma in situ breast cancer diagnosed through population-based breast cancer screening program experience a lower risk of readmissions and complications than those diagnosed outside these programs. These findings can help aid women and health professionals make informed decisions regarding screening.

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Relationship between mammographic screening intervals and size and histology of ductal carcinoma in situ.

American Journal of Roentgenology ◽

10.2214/ajr.172.2.9930774 ◽

1999 ◽

Vol 172 (2) ◽

pp. 313-317 ◽

Cited By ~ 18

Author(s):

K L Carlson ◽

M A Helvie ◽

M A Roubidoux ◽

C G Kleer ◽

H A Oberman ◽

...

Keyword(s):

Ductal Carcinoma In Situ ◽

Carcinoma In Situ ◽

Ductal Carcinoma ◽

Mammographic Screening ◽

Screening Intervals

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Risk factors for non-invasive and invasive local recurrence in patients with ductal carcinoma in situ

Breast Cancer Research and Treatment ◽

10.1007/s10549-013-2539-5 ◽

2013 ◽

Vol 139 (2) ◽

pp. 453-460 ◽

Cited By ~ 33

Author(s):

Laura C. Collins ◽

Ninah Achacoso ◽

Reina Haque ◽

Larissa Nekhlyudov ◽

Suzanne W. Fletcher ◽

...

Keyword(s):

Risk Factors ◽

Local Recurrence ◽

Ductal Carcinoma In Situ ◽

Carcinoma In Situ ◽

Ductal Carcinoma ◽

Non Invasive

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Ductal lavage cytology in patients with ductal carcinoma in situ (DCIS).

Journal of Clinical Oncology ◽

10.1200/jco.2013.31.26_suppl.89 ◽

2013 ◽

Vol 31 (26_suppl) ◽

pp. 89-89

Author(s):

Andrea Behr ◽

Edi Brogi ◽

Alexandra S. Heerdt ◽

Kimberly J. Van Zee ◽

Leslie L. Montgomery

Keyword(s):

Operating Room ◽

Ductal Carcinoma In Situ ◽

Carcinoma In Situ ◽

Ductal Carcinoma ◽

Ductal Lavage ◽

Medical Treatments ◽

Invasive Ductal Cancer ◽

Mastectomy Specimen ◽

Lavage Cytology

89 Background: Ductal lavage (DL) is a procedure used to obtain epithelial cells from a breast duct for cytologic evaluation. Previous studies have investigated DL as a potential screening procedure for women at high-risk for cancer. The data, however, does not support a significant correlation between lavage cytology and the presence of invasive carcinoma. It is postulated that an invasive cancer causes destruction of the involved ducts which prevents direct sampling of the malignant intraductal cells. Because ductal carcinoma in-situ (DCIS) does not disrupt the breast ductal structure, DL may yield a more representative cytologic sample in patients with DCIS. Methods: Ductal lavage was performed in the affected breast of 32 women undergoing mastectomy with preoperative diagnosis of DCIS. The lavage procedure was done in the operating room after induction of general anesthesia by cannulating a duct yielding fluid on the nipple and instilling saline which was then aspirated and sent for cytologic evaluation. A cytopathologist classified each sample as insufficient cellular quantity, benign, mild atypia, marked atypia, or malignant. Results: A successful lavage was obtained from 22 (69%) of 32 women undergoing DL in the operating room. Of these 22 cytology samples; 4(18%) had an insufficient cellular quantity, 4 (18%) had mild atypia, 6 (27%) had marked atypia, and 8 (36%) had malignant cells. Twenty one of these 22 women had DCIS in the surgical mastectomy specimen. Seven (32%) patients were found to have invasive ductal cancer in addition to DCIS on final pathology. Conclusions: Our results show that DL reveals markedly atypical or malignant cytology in 64% of patients with DCIS who underwent a successful lavage procedure. As an office procedure, DL may have the ability to obtain viable ductal carcinoma cells repeatedly over time in the same patient. Thus, DL may have an important role in patients with DCIS who are involved in window trial studies investigating response to medical treatments prior to surgical intervention. Further studies to evaluate the reproducibility of the ductal lavage results in patients with DCIS are needed to confirm these findings.

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