scholarly journals Mesenchymal stem cells cultured in serum-free medium ameliorate experimental peritoneal fibrosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kohei Nagasaki ◽  
Ayumu Nakashima ◽  
Ryo Tamura ◽  
Naoki Ishiuchi ◽  
Kiyomasa Honda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Conditioned Medium ◽  
In Vitro Experiments ◽  
Serum Free Medium ◽  
Free Medium ◽  
Peritoneal Fibrosis ◽  
Serum Free ◽  
Tgf Β1

AbstractBackgroundMesenchymal stem cells (MSCs) provide potential treatments for peritoneal fibrosis. However, MSCs cultured in media containing serum bring risks of infection and other problems. In this study, we compared the effect of human MSCs in serum-free medium (SF-MSCs) on peritoneal fibrosis with that of MSCs cultured in medium containing 10% fetal bovine serum (10%MSCs).MethodsPeritoneal fibrosis was induced by intraperitoneally injecting 0.1% chlorhexidine gluconate (CG). SF-MSCs or 10%MSCs were intraperitoneally administered 30 min after the CG injection. Ten days after the CG and MSC injections, we performed histological analyses and peritoneal equilibrium testing. In the in vitro experiments, we used transforming growth factor (TGF)-β1-stimulated human peritoneal mesothelial cells incubated in conditioned medium from MSCs to examine whether the SF-MSCs showed enhanced ability to produce antifibrotic humoral factors.ResultsHistological staining showed that the SF-MSCs significantly suppressed CG-induced cell accumulation and thickening compared with that of the 10%MSCs. Additionally, the SF-MSCs significantly inhibited mesenchymal cell expression, extracellular matrix protein deposition and inflammatory cell infiltration. Peritoneal equilibration testing showed that compared with administering 10%MSCs, administering SF-MSCs significantly reduced the functional impairments of the peritoneal membrane. The in vitro experiments showed that although the conditioned medium from MSCs suppressed TGF-β1 signaling, the suppression did not significantly differ between the SF-MSCs and 10%MSCs.ConclusionsSerum-free culture conditions can enhance the antifibrotic abilities of MSCs by suppressing inflammation. Administering ex vivo expanded SF-MSCs may be a potential therapy for preventing peritoneal fibrotic progression.

scholarly journals Serum-Free Medium Enhances the Immunosuppressive and Antifibrotic Abilities of Mesenchymal Stem Cells Utilized in Experimental Renal Fibrosis

2018 ◽  
Vol 7 (12) ◽  
pp. 893-905 ◽  
Author(s):  
Ken Yoshida ◽  
Ayumu Nakashima ◽  
Shigehiro Doi ◽  
Toshinori Ueno ◽  
Tomoe Okubo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Renal Fibrosis ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Comparative Study of the Biological Characteristics of Serum-Free and Fetal Bovine Serum-Contained Medium Cultured Umbilical Cord-Derived Mesenchymal Stem Cells

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 4737-4737 ◽  
Author(s):  
Guanghua Chen ◽  
Ting Yang ◽  
Man Qiao ◽  
Huiwen Liu ◽  
Wu Depei
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
T Cell ◽  
Umbilical Cord ◽  
Complete Medium ◽  
Human Umbilical Cord ◽  
Serum Free Medium ◽  
Free Medium ◽  
Early Passage ◽  
Serum Free

Abstract Abstract 4737 Objective: To compare the difference of biological characteristics between human umbilical cord-derived mesenchymal stem cells (UC-MSC) cultured by serum free medium and fetal bovine serum-contained complete medium and to create a xenogeneic protein-free UC-MSC culture system. Methods: Healthy human umbilical cord segments were digested with collagenase. Umbilical cord-derived mesenchymal stem cells were cultured by serum free MesenCult-XF medium and FBS-based αMEM complete medium. We analysed the morphology, immunophenotype, expansion potential, trilineage differentiation potential, karyotype and immunosuppression of early passage of UC-MSC. Results: The average cell diameters of UC-MSC in suspension cultured by serum free medium and FBS-based medium are 26 (18–39) μm and 35 (20–61) μm, respectively. Cell expansion folds with serum free medium and FBS-based medium were (5.2±0.2) and (3.5±0.1) in the first five passage, respectively. The expansion potential of MSCs was significantly higher with serum free medium compared to FBS-based medium (P<0.05). A panel of markers as CD29, CD44, CD90, CD73, CD105 and HLA-ABC were expressed by human UC-MSC. Hematopoietic lineage markers CD34, CD45 and HLA-DR were not detectable on UC-MSC. The cpm were (4.57±0.14)×104, (2.04±0.16)×104 and(0.42±0.04)×104 when serum free medium cultured MSCs were added to the cultures at ratios MSCs/T cell of 1:100, 1:10 and 1:5. While the cpm were (4.57±0.14)×104, (2.04±0.16)×104 and(0.42±0.04)×104when serum free medium cultured UC-MSCs were added to the cultures. The immunosuppressive potential of serum free medium-cultured UC-MSC was higher than that of serum-contained medium cultured UC-MSC at three different ratios MSC/T cell (P<0.05). Conclusion Compare with serum-contained medium cultured early passage of UC-MSC, the cell diameter of serum free medium cultured MSCs was smaller and the expansion potential was higher. No xenogeneic proteins were presented in UC-MSC preparation when UC-MSC was cultured with serum free medium. Human UC-MSC suppresses T-cell proliferation in a dose-dependent manner. The immunosuppressive potential of UC-MSC was higher when cultured in serum free medium compared with FBS-based medium. Disclosures: No relevant conflicts of interest to declare.

Ex Vivo Expansion of Human Marrow Mesenchymal Stem Cells: A Serum-Free Medium Compared to Classical α-MEM Medium.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4257-4257 ◽  
Author(s):  
Nathalie Meuleman ◽  
Tatiana Tondreau ◽  
Alain Delforge ◽  
Marielle Dejeneffe ◽  
Martine Massy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture Conditions ◽  
Adherent Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Marrow Mesenchymal Stem Cells ◽  
Serum Substitute ◽  
Cells Cultured

Abstract BACKGROUND: Bone Marrow mesenchymal stem cells (MSC) are pluripotent cells that have the capacity to differentiate into several tissue lineages and also the ability to support hematopoiesis. Their use in gene and cell therapy requires their in vitro expansion. Maintenance and proliferation of MSC are strongly dependent on the culture conditions such as properties of the bovine serum added in the culture medium. However, duration and culture conditions are critical for the successful clinical use of MSC. OBJECTIVE: We have evaluated the efficiency of a commercial serum-free medium (UltraCulture, Bio-Whittaker, Walkersville, MD) supplemented with a serum substitute (Ultroser, BioSepra, Cergy-Saint-Christophe, France) in order to work without FBS and to have a more constant composition. This medium (UC) was compared to the classical medium a-MEM containing 10% FBS (Invitrogen, Merelbeke, Belgium). METHODS: BM-mononuclear cells collected from 11 healthy donors were plated in petri dishes at a concentration of 105/cm2. After 3 days, non adherent cells were removed and culture media were added to adherent cells which were maintained at 37°C until they reached confluence. MSC expansion was analysed after the primoculture (PM) and after the first passage (P1). CFU-F (colony forming units-fibroblastic) number, phenotypic analysis and differentiation potential were also evaluated. RESULTS::The mean culture duration was 13±2 and 8±2 days respectively for PM and P1 but the confluence was reached more rapidly for cells cultured in UC. After PM, 0.35x106 and 1.21x106 cells were obtained for MEM and UC respectively (p<0.005). Moreover, around 20% of cells cultured in MEM were CD45+ while the level of CD45+ cells was frequently < 5% in UC indicating that UC medium favored the rapid elimination of hematopoietic cells. After P1, the expansion rate was significantly higher in UC than MEM: respectively 5.13 ± 1.2 and 22.6 ± 3 (p<0.0005). The numbers of CFU-F were always higher in UC demonstrating the enhanced proliferation in serum-free medium. The phenotype of MSC was similar in the both media: SH2+, SH3+, CD44+, CD45−, CD34−, HLA-DR−. More importantly, these cells remained able to differentiate into osteocytes, chondrocytes, adipocytes and neuron-like cells in the both media confirming their multipotentiality. CONCLUSIONS: Our data strongly support UltraCulture medium supplemented with a serum substitute as an optimal medium for MSC culture. Indeed, it allows a better cell expansion, better proliferation and preserves multipotentiality. This medium, reducing culture period and containing low concentration of serum substitute, is of major interest for clinical production of MSC.

scholarly journals The Effectivity of the Novel Serum-free Medium STK2 for Proliferating Human Mesenchymal Stem Cells

2009 ◽  
Vol 129 (3) ◽  
pp. 381-384 ◽  
Author(s):  
Itaru ISHIKAWA ◽  
Rumi SAWADA ◽  
Yukio KATO ◽  
Koichiro TSUJI ◽  
Jinchang SHAO ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
The Novel ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Conditioned serum-free medium from umbilical cord mesenchymal stem cells has anti-photoaging properties

2013 ◽  
Vol 35 (10) ◽  
pp. 1707-1714 ◽  
Author(s):  
Qiuying Liu ◽  
Zhenyu Luo ◽  
Shaoqing He ◽  
Xinlei Peng ◽  
Sheng Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

Growth, differentiation capacity, and function of mesenchymal stem cells expanded in serum-free medium developed via combinatorial screening

2013 ◽  
Vol 319 (10) ◽  
pp. 1409-1418 ◽  
Author(s):  
Kirsten Crapnell ◽  
Rainer Blaesius ◽  
Abel Hastings ◽  
Donald P. Lennon ◽  
Arnold I. Caplan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Combinatorial Screening ◽  
Serum Free ◽  
Differentiation Capacity ◽  
And Function
Sign in / Sign up

Export Citation Format

Share Document