scholarly journals Evaluation of survivin expression in prostate specimens of patients with prostate adenocarcinoma and benign prostate hyperplasia underwent transurethral resection of the prostate or prostatectomy

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Mansooreh Eslami ◽  
Tahere Khamechian ◽  
Tahere Mazoochi ◽  
Hasan Ehteram ◽  
Mojtaba Sehat ◽  
...  
Keyword(s):  
Transurethral Resection ◽  
Benign Prostate Hyperplasia ◽  
Survivin Expression ◽  
Prostate Adenocarcinoma ◽  
Prostate Hyperplasia ◽  
Benign Prostate

C30 DETECTION OF METHYLATION OF GSTP1, E-CAD AND PTGS2 IN PROSTATE ADENOCARCINOMA AND BENIGN PROSTATE HYPERPLASIA

2010 ◽  
Vol 9 (6) ◽  
pp. 626-627 ◽  
Author(s):  
P. Tilandyová ◽  
S. Grobarčiková ◽  
K. Kajo ◽  
J. Kliment ◽  
Z. Lasabová ◽  
...  
Keyword(s):  
Benign Prostate Hyperplasia ◽  
Prostate Adenocarcinoma ◽  
Prostate Hyperplasia ◽  
Benign Prostate

scholarly journals Overexpression of MiR-155-5p and increased number of macrophage population in precancerous prostatic disease

2020 ◽  
Vol 11 (2) ◽  
pp. 85-91
Author(s):  
Rachma Greta Putri ◽  
Sari Eka Pratiwi ◽  
Didik Setyo Heriyanto ◽  
Danarto Danarto ◽  
Indwiani Astuti ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Reverse Transcriptase ◽  
Benign Prostate Hyperplasia ◽  
Intraepithelial Neoplasia ◽  
Prostate Adenocarcinoma ◽  
Prostatic Intraepithelial Neoplasia ◽  
High Grade ◽  
Prostate Hyperplasia ◽  
Macrophage Population ◽  
Benign Prostate

Latar Belakang: Gangguan regulasi mikroRNA(miR) dan inflamasi kronik dapat mengubah tumor menjadi karsinoma dan kanker dengan metastasis melalui perubahan seluler dan genomik. Lesi prekanker memiliki peluang 33,3 persen menjadi kanker. Penelitian ini bertujuan untuk mengkaji peran miR-155-5p terhadap mRNA SOCS1 dan populasi makrofag terhadap progresivitas penyakit yang berhubungan dengan Benign Prostate Hyperplasia (BPH), High Grade Prostatic Intraepithelial Neoplasia (HGPIN), dan Prostate Adenocarcinoma (PRAD). Metode: Penelitian ini merupakan penelitian potong lintang dengan 3 kelompok, yaitu BPH,HGPIN, dan PRAD. Sampel jaringan didapatkan dari Tindakan TURP. Ekspresi miR-155 dianalisis menggunakan qPCR dan dikalkulasi menggunakan metode Livak. Ekspresi mRNA SOCS-1 dianalisis menggunakan reverse transcriptase PCR. Penanda pan makrofag, anti CD-68 monoclonal antibody(MoAb) digunakan untuk mendeteksi populasi makrofag pada jaringan dengan imunohistokimia. Hasil: Ekspresi miR-155 lebih tinggi pada HGPIN dibandingkan BPH dan PRAD (p=0,14). Ekspresi mRNA SOCS1 pada HGPIN paling rendah diantara ketiga sampel (p=0,96). Terdapat korelasi negative antara miR-155 dan mRNA SOCS1 (p=0,02). Terdapat peningkatan persentase populasi makrofag yang signifikan pada HGPIN (6,03 persen) dibandingkan BPH (0.89 persen) dengan p=0,00. Kesimpulan: Pada penelitian ini, terdapat perubahan persentase makrofag dan miR-155 pada HGPIN. Variasi ekspresi miR-155 dan persentase populasi makrofag dapat disebabkan karena perubahan epigenetik. Oleh sebab itu, perlu penelitian lebih lanjut untuk memvalidasi hasil tersebut dan memahami  kemungkinan menjadi biomarker pada penyakit prekanker pada prostat. Kata Kunci: Prostatic Intaepithelial Neoplasia, miR-155, Makrofag   Abstract   Background: Impaired microRNA(miR) regulation and chronic inflammation could transform tumors into carcinoma and cancer by metastasis through cellular and genomic changes. Precancerous lesions have a 33.3 percent chance of becoming cancerous. This study investigated the role of miR-155 related to SOCS1 mRNA and macrophage population in disease progression associated  with Benign Prostate Hyperplasia (BPH), High-Grade Prostatic Intraepithelial Neoplasia (HGPIN), and Prostate Adenocarcinoma (PRAD). Methods: This was a cross-sectional study using three groups of samples, namely BPH, HGPIN, and PRAD. Tissue samples were obtained from TURP Action. The expression of miR-155 was analyzed using real-time qPCR and calculated using the Livak method. The expression of SOCS1 mRNA was analyzed using reverse transcriptase PCR. The macrophage pan-marker, anti-CD68 monoclonal antibody (MoAb), was used to detect macrophage population in tissues by immunohistochemistry. Results: The expression of miR-155 was higher in HGPIN than BPH and PRAD (p=0.14). The expression of SOCS1 mRNA in HGPIN was the lowest among the three samples (p=0.96). There was a negative correlation between miR-155 and SOCS1 mRNA (p=0.02). There was a significant increase in the percentage of the macrophage population in HGPIN (6.03 percent) compared to BPH (0.89 percent) with p=0.00. Conclusion: In this study, there were changes in the percentage of macrophage and miR-155 in HGPIN. The variation in miR-155 expression and the percentage of the macrophage may be caused by epigenetic changes. Therefore, further research is needed to validate these results and understand the possibility of being a biomarker in precancerous disease of the prostate. Keywords: Prostatic Intraepithelial Neoplasia, miR-155, Macrophage  

Dynamic instrumented palpation – a new method for soft tissue quality assessment: application to prostate disease diagnosis

2017 ◽  
Vol 231 (12) ◽  
pp. 1101-1115 ◽  
Author(s):  
TH Jimmy Yang ◽  
Simon Phipps ◽  
Steve KW Leung ◽  
Robert L Reuben ◽  
Fouad K Habib ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mechanical Properties ◽  
Soft Tissue ◽  
Transurethral Resection ◽  
Benign Prostate Hyperplasia ◽  
Dynamic Mechanical Properties ◽  
Prostate Hyperplasia ◽  
Dynamic Mechanical ◽  
Benign Prostate

The objective is to establish the feasibility of using dynamic instrumented palpation, a novel technique of low-frequency mechanical testing, applied here to diagnose soft tissue condition. The technique is applied, in vitro, to samples of excised prostate gland affected by benign prostate hyperplasia and/or prostate cancer. Particular attention is paid to the relationship between the histological structure of the tissue and the dynamic mechanical properties in an attempt to separate patient-specific aspects from histopathological condition (i.e. prostate cancer or benign prostate hyperplasia). The technique is of clinical interest because it is potentially deployable in vivo. Prostate samples were obtained from a total of 36 patients who had undergone transurethral resection of the prostate to relieve prostatic obstruction and 4 patients who had undergone radical cystoprostatectomy for bladder cancer. Specimens (chips) recovered from transurethral resection of the prostate were of nominal size 5 mm × 8 mm and thicknesses between 2 and 4 mm, whereas those from the cystoprostatectomy were in the form of transverse slices of thickness approximately 6 mm. Specimens were mechanically tested by a controlled strain cyclic compression technique, and the resulting dynamic mechanical properties expressed as the amplitude ratio and phase difference between the cyclic stress and cyclic strain. After mechanical testing, the percentage areas of glandular and smooth muscle were measured at each probe point. Good contrast between the dynamic modulus of chips from benign prostate hyperplasia and prostate cancer patients was demonstrated, and absolute values similar to those published by other authors are reported. For the slices, modulus values were considerably higher than for chips, and good in-patient mechanical contrast was revealed for predominantly nodular and predominantly stromal areas. Extending this classification between patients required pattern recognition techniques. Overall, the study has demonstrated that dynamic mechanical properties can potentially be used for diagnosis of prostate condition using in vivo measurements.

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