prostatic intraepithelial neoplasia
Recently Published Documents


TOTAL DOCUMENTS

656
(FIVE YEARS 38)

H-INDEX

66
(FIVE YEARS 2)

2021 ◽  
Author(s):  
Juan Morote ◽  
Iván Schwartzmann ◽  
Anna Celma ◽  
Sarai Roche ◽  
Inés M. Torres ◽  
...  

2021 ◽  
pp. 66-69
Author(s):  
Prashant Bhardwaj ◽  
Sumit Nandy ◽  
Keya Basu ◽  
Manjari Kumari ◽  
Dilip Karmakar

Objectives- Prostate is a bromusculoglandular structure situated at the neck of urinary bladder. It enlarges due to benign hyperplasia of prostate (BHP), prostatic intraepithelial neoplasia (PIN) or adenocarcinoma. Enlargement of prostate is associated with raised serum level of prostate-specic antigen (PSA) and altered expression of estrogen receptor (ER) and progesterone receptor (PR). The aim of our study is to correlate the histopathology, PSA levels and altered expression of ER and PR by immunohistochemistry in different prostatic growth lesions. Methodology- Patients diagnosed as having prostatic growth were enrolled and their serum PSA levels were noted. Histopathological examination and immunohistochemical analysis of prostatic tissues for ER and PR were carried out to nd out correlation of different type of growth with serum PSA level and expression of ER and PR. Results- A total 96 cases studied of them 61(63.54%) patients presented with BHP, 20(20.83%) patients with BHP with chronic prostatitis, 3 patients presented with metaplastic changes, 5 cases with of PIN and 6 patients presented with adenocarcinoma with different Gleason score. PR expression positivity in epithelial cells and stromal cells of BHP cases were 51(83.6%) and 53(86.88%) respectively. Patients presented with adenocarcinoma showed only 33.33 %( 2cases) positivity in epithelial cells and 50% (3cases) positivity in stromal cells. Serum PSA level were signicantly higher in adenocarcinoma patients as compare to BHP patients. Conclusion- By observing these ndings it can be suggested that and antiprogesterone therapy may be helpful in the treatment of prostatic adenocarcinoma.


Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3608
Author(s):  
Liliana Rounds ◽  
Ray B. Nagle ◽  
Andrea Muranyi ◽  
Jana Jandova ◽  
Scott Gill ◽  
...  

Glyoxalase 1 (GLO1) is an enzyme involved in the detoxification of methylglyoxal (MG), a reactive oncometabolite formed in the context of energy metabolism as a result of high glycolytic flux. Prior clinical evidence has documented GLO1 upregulation in various tumor types including prostate cancer (PCa). However, GLO1 expression has not been explored in the context of PCa progression with a focus on high-grade prostatic intraepithelial neoplasia (HGPIN), a frequent precursor to invasive cancer. Here, we have evaluated GLO1 expression by immunohistochemistry in archival tumor samples from 187 PCa patients (stage 2 and 3). Immunohistochemical analysis revealed GLO1 upregulation during tumor progression, observable in HGPIN and PCa versus normal prostatic tissue. GLO1 upregulation was identified as a novel hallmark of HGPIN lesions, displaying the highest staining intensity in all clinical patient specimens. GLO1 expression correlated with intermediate–high risk Gleason grade but not with patient age, biochemical recurrence, or pathological stage. Our data identify upregulated GLO1 expression as a molecular hallmark of HGPIN lesions detectable by immunohistochemical analysis. Since current pathological assessment of HGPIN status solely depends on morphological features, GLO1 may serve as a novel diagnostic marker that identifies this precancerous lesion.


Author(s):  
Anne Offermann ◽  
Vincent Joerg ◽  
Marie C. Hupe ◽  
Finn Becker ◽  
Marten Müller ◽  
...  

2021 ◽  
Author(s):  
Romildo Luciano da Silva ◽  
Ingrid Tavares de Lima ◽  
Francisco Luis Almeida Paes ◽  
Sandra Maria Souza da Silva ◽  
Ana Pavla A. D. Gurgel ◽  
...  

Matrix metalloproteinases (MMP) have been identified as biomarkers for several diseases, including cancer. MMP-26 is constitutively expressed in some cancer cells of epithelial origin. Despite this, there is a lack of studies regarding the expression of MMP-26 on prostatic carcinoma. Here, we investigate the expression of the MMP-26 peptide in benign and malign prostatic tissues. For this, 150 specimens, including atrophy (N = 25), prostatic intraepithelial neoplasia (PIN) (N = 25), benign prostatic hyperplasia (BPH) (N = 50), and prostatic adenocarcinoma (PA) (N = 50), were immunohistochemically (IHC) examined for the expression of MMP-26. MMP-26 expression was positive in 70 (46.7%) out of the 150 samples, being more prevalent in the PA group (46/50 cases,92%), followed by PIN (22/25 cases, 88%). The BPH group showed only 2/50 (4%) positive cases, and the atrophy group showed no reactivity. ROC curve analysis showed that MMP-26 immunoexpression had a higher area under the curve between PA vs atrophy+PIN+BPH (AUC=0.94; 95% CI 0.9-0.98), PA+PIN vs atrophy+BPH (AUC=0.97; 95% CI 0.94-0.99) and PA vs atrophy+BPH (AUC=0.97; 95% CI 0.95-1.00) groups. In addition, the expression and intensity of the MMP-26 reaction showed a significant association with total PSA values (P=0.001). Our results showed that MMP-26 immunoexpression was useful to differentiate a group of benign and malignant samples in prostate tumors. This characteristic could assist in the predictive assessment and, consequently, in the development of new strategies for the diagnosis, prognosis, and treatment of prostate cancer.


2020 ◽  
Vol 11 (2) ◽  
pp. 85-91
Author(s):  
Rachma Greta Putri ◽  
Sari Eka Pratiwi ◽  
Didik Setyo Heriyanto ◽  
Danarto Danarto ◽  
Indwiani Astuti ◽  
...  

Latar Belakang: Gangguan regulasi mikroRNA(miR) dan inflamasi kronik dapat mengubah tumor menjadi karsinoma dan kanker dengan metastasis melalui perubahan seluler dan genomik. Lesi prekanker memiliki peluang 33,3 persen menjadi kanker. Penelitian ini bertujuan untuk mengkaji peran miR-155-5p terhadap mRNA SOCS1 dan populasi makrofag terhadap progresivitas penyakit yang berhubungan dengan Benign Prostate Hyperplasia (BPH), High Grade Prostatic Intraepithelial Neoplasia (HGPIN), dan Prostate Adenocarcinoma (PRAD). Metode: Penelitian ini merupakan penelitian potong lintang dengan 3 kelompok, yaitu BPH,HGPIN, dan PRAD. Sampel jaringan didapatkan dari Tindakan TURP. Ekspresi miR-155 dianalisis menggunakan qPCR dan dikalkulasi menggunakan metode Livak. Ekspresi mRNA SOCS-1 dianalisis menggunakan reverse transcriptase PCR. Penanda pan makrofag, anti CD-68 monoclonal antibody(MoAb) digunakan untuk mendeteksi populasi makrofag pada jaringan dengan imunohistokimia. Hasil: Ekspresi miR-155 lebih tinggi pada HGPIN dibandingkan BPH dan PRAD (p=0,14). Ekspresi mRNA SOCS1 pada HGPIN paling rendah diantara ketiga sampel (p=0,96). Terdapat korelasi negative antara miR-155 dan mRNA SOCS1 (p=0,02). Terdapat peningkatan persentase populasi makrofag yang signifikan pada HGPIN (6,03 persen) dibandingkan BPH (0.89 persen) dengan p=0,00. Kesimpulan: Pada penelitian ini, terdapat perubahan persentase makrofag dan miR-155 pada HGPIN. Variasi ekspresi miR-155 dan persentase populasi makrofag dapat disebabkan karena perubahan epigenetik. Oleh sebab itu, perlu penelitian lebih lanjut untuk memvalidasi hasil tersebut dan memahami  kemungkinan menjadi biomarker pada penyakit prekanker pada prostat. Kata Kunci: Prostatic Intaepithelial Neoplasia, miR-155, Makrofag   Abstract   Background: Impaired microRNA(miR) regulation and chronic inflammation could transform tumors into carcinoma and cancer by metastasis through cellular and genomic changes. Precancerous lesions have a 33.3 percent chance of becoming cancerous. This study investigated the role of miR-155 related to SOCS1 mRNA and macrophage population in disease progression associated  with Benign Prostate Hyperplasia (BPH), High-Grade Prostatic Intraepithelial Neoplasia (HGPIN), and Prostate Adenocarcinoma (PRAD). Methods: This was a cross-sectional study using three groups of samples, namely BPH, HGPIN, and PRAD. Tissue samples were obtained from TURP Action. The expression of miR-155 was analyzed using real-time qPCR and calculated using the Livak method. The expression of SOCS1 mRNA was analyzed using reverse transcriptase PCR. The macrophage pan-marker, anti-CD68 monoclonal antibody (MoAb), was used to detect macrophage population in tissues by immunohistochemistry. Results: The expression of miR-155 was higher in HGPIN than BPH and PRAD (p=0.14). The expression of SOCS1 mRNA in HGPIN was the lowest among the three samples (p=0.96). There was a negative correlation between miR-155 and SOCS1 mRNA (p=0.02). There was a significant increase in the percentage of the macrophage population in HGPIN (6.03 percent) compared to BPH (0.89 percent) with p=0.00. Conclusion: In this study, there were changes in the percentage of macrophage and miR-155 in HGPIN. The variation in miR-155 expression and the percentage of the macrophage may be caused by epigenetic changes. Therefore, further research is needed to validate these results and understand the possibility of being a biomarker in precancerous disease of the prostate. Keywords: Prostatic Intraepithelial Neoplasia, miR-155, Macrophage  


Sign in / Sign up

Export Citation Format

Share Document