scholarly journals The synthesis characterization of historical novel azo pigments: implications for conservation science

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Suzanne Quillen Lomax ◽  
Joseph F. Lomax
Keyword(s):  
Historical Novel ◽  
Substituent Effects ◽  
Reflectance Spectroscopy ◽  
Model Systems ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Substituted Anilines ◽  
Organic Pigments ◽  
Visible Reflectance

AbstractA series of synthetic organic pigments (SOP’s) with azo-containing linkages have been synthesized and characterized by Fourier transform infrared (FTIR) and Raman spectroscopies and laser desorption ionization mass spectrometry (LDI-MS). These pigments include representatives of the chemical classes arylide (Hansa) yellow, diarylide yellow and oranges, and Naphthol AS. Certain of these (PR7, PR10, PR11, PR18, PR19, PO14, PO15, PO16, PY49, PY98, PY106, PY113, PY124, PY203) are historical pigments, that is they were manufactured but subsequently were removed from production. Of these, some have been brought back into limited production. In addition to the above-mentioned pigments, 18 products of azo-linkage reactions formed between N,N′-bis(acetoacetyl)-o-toluidine and substituted anilines have been synthesized and characterized by the methods listed above as well as by visible reflectance spectroscopy. While not commercially available azo pigments, these colorants will serve as model systems to study substituent effects on pigment color.

scholarly journals A binary matrix for the rapid detection and characterization of small-molecule cardiovascular drugs by MALDI-MS and MS/MS

2018 ◽  
Vol 10 (6) ◽  
pp. 572-578 ◽  
Author(s):  
Jinlan Dong ◽  
Wenjing Ning ◽  
Daniel J. Mans ◽  
Jamie D. Mans
Keyword(s):  
Small Molecule ◽  
Hydroxycinnamic Acid ◽  
Cardiovascular Drugs ◽  
Maldi Ms ◽  
Negative Ion ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Binary Matrix ◽  
Negative Ion Mode

A mixture of α-cyano-4-hydroxycinnamic acid and 1,5-diaminonaphthalene was discovered as a novel binary matrix for the qualitative analysis of 14 small-molecule (∼250–550 Da) cardiovascular drugs by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and MS/MS in either positive or negative ion mode.

Direct laser desorption/ionization mass spectrometry characterization of some aromatic lanthanide carboxylates

2008 ◽  
Vol 451 (1-2) ◽  
pp. 410-413 ◽  
Author(s):  
Oxana V. Kotova ◽  
Svetlana V. Eliseeva ◽  
Vladislav V. Lobodin ◽  
Albert T. Lebedev ◽  
Natalia P. Kuzmina
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Direct Laser

265 MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY (MALDI-MS) CHARACTERIZATION OF SPERM LIPID PROFILES OF BULLS WITH DIFFERENT CAPACITIES OF EMBRYO IN VITRO PRODUCTION

2010 ◽  
Vol 22 (1) ◽  
pp. 289
Author(s):  
C. R Ferreira ◽  
J. C. Borges ◽  
L. F. A. Santos ◽  
F. C. Gozzo ◽  
P. H. Franscechini ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Lipid Profiles ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
In Vitro Production ◽  
Vitro Production ◽  
Matrix Assisted Laser

Matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) has been applied to study sperm lipid profiles. Lipids are known to play a crucial role in sperm membrane physico-chemical behavior during cryopreservation. In this work, we show the results of characterization of sperm lipid profiles from 2 bulls with different capacities of in vitro embryo production by MALDI-MS direct analysis. The bull capacities judged by the rate of blastocyst formation after IVP with semen from seven different ejaculates per each animal were 19.1 and 35.3% for bulls 1 and 2, respectively (P < 0.05). For MALDI-MS analysis, frozen semen from each ejaculate was thawed in water at 25°C for 40 s. Sperm was washed 3 times by centrifugatin in 1 mL of PBS at 3000 × g for 10 min. Samples were stored at -20°C in 200 μL of methanol:PBS (vol/vol) solution until analysis. A Synapt HDMS mass spectrometer (Waters Corp., Milford, MA, USA) equipped with a MALDI was used. All spectra were collected for 45 s in the positive ion mode at the mass range of m/z 450 to 1200. The volume of 1 μL of the semen pellet was spotted in the target plate and allowed to dry. Afterward, 1 μL of 2,5-dihydroxybenzoic acid (DHB) was added as matrix. The 50 most intense monoisotopic ions were considered for principal component analysis (PCA). Values of m/z and relative ion intensities were processed using the software Pirouette v.3.11 (Infometrix, Woodinville, WA, USA). Direct MALDI-MS analysis of bulls 1 and 2 spermatozoa with no extraction provided informative spectra containing either [M + Na]+ or [M + H]+ ions characteristic of sphingomyelins, such as m/z 753.6 for SM 18:0, phosphocholines (m/z 780.6 for PC 34 : 2; 782.6 for PC 34 : 1; 806.6 for PC 36 : 6; 808.6 for 36 :2; 828.6 for PC38 : 6; and 830.6 for PC38 : 5), plasmalogens (m/z 790.6 for 1-palmitenyl-2-docosahexanoyl-GPC and 814.6 for 1-palmityl-2-docosaheaenoyl-GPC); and triacylglycerols (m/z 881.7 for sn-glycerol-palmitoleate-oleate-oleate). PCA showed clear separation between bulls 1 and 2 ejaculates, indicating that each bull presented a characteristic and reproducible (from different ejaculates) profiles. Differences in the relative intensities of the ions mentioned above contributed for bulls 1 and 2 differentiation by PCA. PC1 and PC2 explained 86.5% of the data variance. In conclusion, a fast sample preparation protocol followed by MALDI-MS appears to provide characteristic lipid fingerprints for crude spermatozoa (ejaculates) of bulls with different capacities of embryo in vitro production. Experiments involving a larger and more statically relevant set of samples are underway. We thank the Brazilian research foundations FAPESP (2008/10756-7) and CNPq.

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