Plasma metabolomic profiling of hypertrophic cardiomyopathy patients before and after surgical myectomy suggests postoperative improvement in metabolic function

Background Hypertrophic cardiomyopathy (HCM) is a common inherited heart disorder complicated by left ventricle outflow tract (LVOT) obstruction, which can be treated with surgical myectomy. To date, no reliable biomarkers for LVOT obstruction exist. We hypothesized that metabolomic biomarkers for LVOT obstruction may be detectable in plasma from HCM patients. Methods We conducted metabolomic profiling on plasma samples of 18 HCM patients before and after surgical myectomy, using a commercially available metabolomics platform. Results We found that 215 metabolites were altered in the postoperative state (p-value < 0.05). 12 of these metabolites were notably significant after adjusting for multiple comparisons (q-value < 0.05), including bilirubin, PFOS, PFOA, 3,5-dichloro-2,6-dihydroxybenzoic acid, 2-hydroxylaurate, trigonelline and 6 unidentified compounds, which support improved organ metabolic function and increased lean soft tissue mass. Conclusions These findings suggest improved organ metabolic function after surgical relief of LVOT obstruction in HCM and further underscore the beneficial systemic effects of surgical myectomy.

Salts of purine alkaloids caffeine and theobromine with 2,6-dihydroxybenzoic acid as coformer: structural, theoretical, thermal and spectroscopic studies

The study of various forms of pharmaceutical substances with specific physicochemical properties suitable for putting them on the market is one of the elements of research in the pharmaceutical industry. A large proportion of active pharmaceutical ingredients (APIs) occur in the salt form. The use of an acidic coformer with a given structure and a suitable pK a value towards purine alkaloids containing a basic imidazole N atom can lead to salt formation. In this work, 2,6-dihydroxybenzoic acid (26DHBA) was used for cocrystallization of theobromine (TBR) and caffeine (CAF). Two novel salts, namely, theobrominium 2,6-dihydroxybenzoate, C7H9N4O2 +·C7H5O4 − (I), and caffeinium 2,6-dihydroxybenzoate, C8H11N4O2 +·C7H5O4 − (II), were synthesized. Both salts were obtained independently by slow evaporation from solution, by neat grinding and also by microwave-assisted slurry cocrystallization. Powder X-ray diffraction measurements proved the formation of the new substances. Single-crystal X-ray diffraction studies confirmed proton transfer between the given alkaloid and 26DHBA, and the formation of N—H...O hydrogen bonds in both I and II. Unlike the caffeine cations in II, the theobromine cations in I are paired by noncovalent N—H...O=C interactions and a cyclic array is observed. As expected, the two hydroxy groups in the 26DHBA anion in both salts are involved in two intramolecular O—H...O hydrogen bonds. C—H...O and π–π interactions further stabilize the crystal structures of both compounds. Steady-state UV–Vis spectroscopy showed changes in the water solubility of xanthines after ionizable complex formation. The obtained salts I and II were also characterized by theoretical calculations, Fourier-transform IR spectroscopy (FT–IR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and elemental analysis.

A Possibility to Use Selected Crop Post-Extraction Wastes to Improve the Composition of Cultivated Mushroom Pleurotus citrinopileatus

A cultivated mushroom species, Pleurotus citrinopileatus, is becoming increasingly popular thanks to its attractive colour and medicinal properties. In this study, P. citrinopileatus was grown in a cultivation medium enriched with wheat bran (WB), thymus post-extraction waste (TPEW) and pumpkin post-extraction waste (PPEW) products. The study showed that the post-extraction wastes are a crucial factor determining the accumulation of minerals, the content/profile of low-molecular-weight organic acids (LMWOAs) and phenolic compounds in fruit bodies, thereby increasing their nutritional value. The use of the waste materials significantly increased LMWOAs contents. The sum of LMWOAs under all cultivation mediums increased, especially quinic, malic and citric acids under the 20% PPEW, 25 and 50% TPEW addition. Total phenolic content, phenolic content, as well as the composition and scavenging effect on DPPH radicals, were strongly dependent on the used substrate. The control variant was poor in phenolic compounds, while the supplementation increased the contents and diversity of these metabolites. In the control, only four phenolic compounds were quantified (chlorogenic, gallic, syringic and vanillic acids), while in the supplemented substrates up to 14 different phenolic compounds (caffeic, chlorogenic, p-coumaric, 2,5-dihydroxybenzoic acid, ferulic, gallic, protocatechuic, salicylic, sinapic, syringic, trans-cinnamic and vanillic acids, catechin and rutin).

Flavonoids quantification in Acer negundo L., extracts by hplc analysis

Objective: The identify and quantify, by high performance liquid chromatography,Nflavonoids from leaf and stem extracts of Acer negundo.Design/methodology/approach: Ethanolic extracts of Acer negundo were analysed with high performance liquid chromatography to quantify and identify their major antioxidant flavonoids.Results: Leaf extracts had high concentrations of rutin (34.19 µg/mL) and catechin (33.97 µg/mL), intermediate concentrations of apigenin (19.05 µg/mL), gallic acid (19.04 µg/mL), ferulic acid (17.2 µg/mL) and 2.5 dihydroxybenzoic acid (12.72 µg/mL), and low concentrations of caffeic acid (6.15 µg/mL), quercetin-3-β-glucoside (4.97 µg/mL) and isorhamnetin (4.68 µg/mL). In the stem´s extracts, the highest concentrations were of ferulic acid (7.96 µg/mL), rutin (5.61 µg/mL) and catechin (4.37 µg/mL); medium concentration were identified for isorhamnetin (3.31 µg/mL) and quercetin-3-β-glucoside (2.01 µg/mL) and apigenin (0.79 µg/mL) was identified at the low concentrations. Gallic acid, caffeic acid or 2,5-dihydroxybenzoic acid were not detected.Limitations/implications: Some flavonoids have been identified in other Acer species but have not been identified and quantified in Acer negundo, a Mexicanspecies.Findings/conclusions: For the first time we report gentisic acid in Acer negundo leaf extracts. This analytical method can be standardized to serve as a qualityanalysis of maple tree products.

The importance of Cu × Pb interactions to Lentinula edodes yield, major/trace elements accumulation and antioxidants

Due to the use of various substrates in the production of edible mushrooms which may contain metals, including Cu and Pb, it is important to understand the influence of mutual interactions between them in the process of their accumulation in fruit bodies. For this reason, the effects of Cu, Pb, and Cu × Pb on yield, accumulation of five major elements (Ca, K, Mg, Na and P), trace elements (Cu, Pb and Fe) and some bioactive compounds in Lentinula edodes fruit bodies were studied. Both the metals were added in doses of 0.1 and 0.5 mM (Cu0.1, Cu0.5, Pb0.1, Pb0.5 and their combinations). The addition of the metals resulted in a reduction in size, amount and finally yield of fruit bodies. Depending on the presence of Cu and or Pb and their concentration in the substrate, both antagonism and synergism may occur. The influence on the accumulation of other determining elements was also recorded. Among phenolic compounds, phenolic acids and flavonoids were detected. 2,5-Dihydroxybenzoic acid dominated in fruit bodies in the control variant, Pb0.1, Pb0.5 and all experimental variants enriched with Cu + Pb, while gallic acid was the major phenolic after Cu0.1 and Cu0.5 addition. Only protocatechuic acid content increased in all combinations. A significant decrease of all aliphatic acid contents in comparison to the control variant was observed in the Cu0.1 and Pb0.1 variants. Significant stimulation of aliphatic acid synthesis was recorded in Cu0.5 and Pb0.5 variants and in the mixture of both the metals. The additions pointed to the possible role of the determined molecules in detoxification mechanisms.