scholarly journals Effect of the entomopathogenic fungus, Beauveria bassiana, combined with diatomaceous earth on the red flour beetle, Tribolium castaneum (Herbst) (Tenebrionidae: Coleoptera)

Author(s):  
Muhammad Rizwan ◽  
Bilal Atta ◽  
Misbah Rizwan ◽  
Arshed Makhdoom Sabir ◽  
Zafar Ullah Shah ◽  
...  
2012 ◽  
Vol 4 (3) ◽  
pp. 783-788
Author(s):  
A. M. S. Reza ◽  
M. M. Hossain ◽  
S. Parween

Tribolium castaneum (Herbst) adults were released to choice chambers containing untreated flour in one half and diatomaceous earth (DE)-treated flour in the other half. The doses of DE used were 2-32 mg/g. Females, males and unsexed adults were exposed separately to each dose for 24-, 48-, 72-, 96- and 120-h. DE at 32 mg/g strongly repelled (P<0.001) both sexes and unsexed beetles at all exposure periods. The males showed comparatively more avoidance to DE-treated flour at longer exposures.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i3.9637 J. Sci. Res. 4 (3), 783-788 (2012)


2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Habib-ur Rehman ◽  
Amer Rasul ◽  
Muhammad Aslam Farooqi ◽  
Hafiz Muhammad Usman Aslam ◽  
Beenish Majeed ◽  
...  

Abstract Background The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is a serious pest of stored grain commodities worldwide which results in considerable losses to stored wheat, Triticum aestivum (Linn.). Main body The present laboratory bioassay was carried out to examine the efficiency of the fungus, Beauveria bassiana (Bals.) formulation (Racer TM), and Neem extract (NE), Eucalyptus extract (EE), and Tobacco extract (TE) against 3rd instar larvae of T. castaneum. The fungal formulation was applied at 0.3 × 108, 0.6 × 108, and 0.9 × 108 conidia kg−1 of the crushed wheat grains; as well, it was mixed separately with 5% concentrations of each plant extract under the laboratory conditions of 30 ± 2 °C and 65 ± 5% RH. Mortality rate of the tested larvae was enumerated after regular intervals of times. Mortality of the larvae increased at the highest combined concentrations of the fungal conidial formulation and the plant extracts rather than their single treatments. The highest mortality rate of the larvae (71.32%) was recorded at the highest concentration binary mixture of (Racer TM +NE), whereas relatively the lowest mortality rate (15.54%) was enumerated in the combined treatments of (Racer TM +NE). Furthermore, the highest separate concentration of B. bassiana (0.9 × 108 conidia/ml) persistently resulted in more larval mortality (32.68%) of 3rd instar larvae of T. castaneum than the plant extracts (7.52, 9.89, and 14.61%), respectively. A noticeably greater rate of mycosis and sporulation was counted in the larvae of the insect in separate treatments of B. bassiana than in its combined applications with the plant extracts. The highest mycosis (85.13%) and sporulation (160.12 conidia/ml) was detected in the treatment, where the lowest concentration of B. bassiana (0.3 × 108 conidia/ml) was used, alone. Conclusion Hence, it was concluded that integrated use of these two bio-pesticides plus the fungus can be helpful in the integrated pest management program of T. castaneum.


Author(s):  
Seher Uçar ◽  
Turgut Atay ◽  
Yusuf Yanar

In this work, control capacity of the four isolates (GN22-1, HP15, HP5-2, HP3-1) of entomopathogenic fungus Beauveria bassiana Bals. (Vuill) were evaluated against Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae) adults under laboratory conditions. To test the effect of each of the isolate on adults of T. castaneum were dipped into 1×108 conidia/ml suspension of each isolate. The data for mortality was recorded after 3rd, 5th, 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, and 23rd day. Thirteen days after application, isolates were listed as GN22-1 (72.85%), HP3-1 (48.88%), HP15 (47.37%) and HP5-2 (30.43%) based on the mortality rate they caused. Mortality rate was 83.52% at the end of the 23rd day with isolate GN22-1. While HP3-1 (53.74%) and HP15 (52.24%) caused more than 50% effect at the end of 23 days incubation period, the effect of HP5-2 remained only 32.51%. In addition, LT50 and LT90 rates were also determined. We arrive to the conclusion that especially GN22-1 isolate can has a potential in the control of this insect and may serve an alternative to chemical insecticides.


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