Isolation, identification and biocontrol potential of entomopathogenic nematodes occurring in Purvanchal and Bundelkhand regions of Uttar Pradesh, India

Agricultural soils and open fields from Western Uttar Pradesh (India) were surveyed to determine the presence of entomopathogenic nematodes. From the entomopathogenic nematodes isolated, Heterorhabditis isolates were selected and further characterized using morphological, morphometrical and molecular approaches. The results showed that three isolated nematodes were Heterorhabditis bacteriophora and were associated with Photorhabdus laumondii subsp. clarkei bacteria, while the rests were identified as Heterorhabditis indica. The biocontrol potential of H. bacteriophora against three agricultural pests was evaluated. Nematode infectivity experiments showed that the nematode isolates DH7 and DH8 were highly pathogenic against cotton bollworm (Helicoverpa armigera) and tobacco cutworm (Spodoptera litura), and less pathogenic against white grub (Holotrichia serrata) larvae. This study sets the basis for establishing new biocontrol agents to be used in pest management programs in India.

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Biocontrol potential of indigenous entomopathogenic nematodes from Cameroon against scale insect pest Stictococcus vayssierei and tending ant Anoplolepis tenella

Biological Control ◽

10.1016/j.biocontrol.2020.104321 ◽

2020 ◽

Vol 149 ◽

pp. 104321

Author(s):

Françoise Ngo Kanga ◽

Stefan Hauser ◽

Maurice Moens

Keyword(s):

Entomopathogenic Nematodes ◽

Insect Pest ◽

Scale Insect ◽

Stictococcus Vayssierei ◽

Biocontrol Potential

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Biocontrol potential of entomopathogenic nematodes heterorhabditis and steinernema against pupae and adults of white grubHolotrichia serrata F.

Sugar Tech ◽

10.1007/bf02943567 ◽

2006 ◽

Vol 8 (4) ◽

pp. 268-271 ◽

Cited By ~ 3

Author(s):

C. Sankaranarayanan ◽

N. Somasekhar ◽

B. Singaravelu

Keyword(s):

Entomopathogenic Nematodes ◽

Biocontrol Potential

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Isolation and identification of entomopathogenic nematodes from citrus orchards in South Africa and their biocontrol potential against false codling moth

Journal of Invertebrate Pathology ◽

10.1016/j.jip.2011.07.006 ◽

2011 ◽

Vol 108 (2) ◽

pp. 115-125 ◽

Cited By ~ 87

Author(s):

Antoinette P. Malan ◽

Rinus Knoetze ◽

Sean D. Moore

Keyword(s):

South Africa ◽

Entomopathogenic Nematodes ◽

Codling Moth ◽

Isolation And Identification ◽

False Codling Moth ◽

Citrus Orchards ◽

Biocontrol Potential

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Entomopathogenic nematodes: a potential biocontrol agent against eggplant ash weevil Myllocerus subfasciatus Guerin (Coleoptera: Curculionidae)

Nematology ◽

10.1163/15685411-00002989 ◽

2016 ◽

Vol 18 (6) ◽

pp. 743-750 ◽

Cited By ~ 4

Author(s):

Manjunatha T. Gowda ◽

Jagadeesh Patil ◽

Devindrappa Mansheppa ◽

Vijayakumar Rangasamy ◽

Abraham Verghese

Keyword(s):

Entomopathogenic Nematodes ◽

Developmental Stages ◽

Pupal Stage ◽

Steinernema Carpocapsae ◽

Progeny Production ◽

Potential Efficacy ◽

Potential Biocontrol Agent ◽

Important Pest ◽

Biocontrol Potential ◽

Insight Into

Eggplant ash weevil, Myllocerus subfasciatus, is an important pest of eggplant that attacks other solanaceous crops in Asia and Southeast Asia. The potential efficacy of two species of entomopathogenic nematodes (EPN), Steinernema carpocapsae and Heterorhabditis indica, against third instar and pre-pupal stages of this pest was tested under laboratory and poly-greenhouse conditions. In this present study, both stages were susceptible to EPN species but the pre-pupal stage was more susceptible. Steinernema carpocapsae was the most effective species against pre-pupal stages. In a pot experiment, among the EPN species tested, S. carpocapsae caused significantly greater mortality (20-100%) than H. indica (16-92%) against pre-pupal stages and S. carpocapsae caused 16-92% mortality in third instar larvae, while H. indica caused (12-80%) mortality. Both EPN species were able to reproduce in third instar and pre-pupal stages of ash weevil, but progeny production rate for H. indica was significantly higher than those of S. carpocapsae. Our observations also revealed that efficacy of EPN against M. subfasciatus varies with developmental stages of M. subfasciatus and EPN species; therefore, no generalisation can be made. Our findings provide the first insight into the biocontrol potential of S. carpocapsae and H. indica against third instar and pre-pupal stages of M. subfasciatus.

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Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes

Plants ◽

10.3390/plants10040629 ◽

2021 ◽

Vol 10 (4) ◽

pp. 629

Author(s):

Mahfouz M. M. Abd-Elgawad

Keyword(s):

Extraction Method ◽

Crop Production ◽

Entomopathogenic Nematodes ◽

Production Systems ◽

Cost Effective ◽

Nematode Species ◽

Extraction Methods ◽

The Common ◽

Biocontrol Potential ◽

Plant Parasitic

Plant-parasitic and entomopathogenic nematodes (PPNs and EPNs) are key groups in crop production systems. This study aims at optimizing nematode sampling and extraction methods to benefit integrated pest management (IPM) through (a) management of PPNs and (b) use of EPNs. The impacts of these methods on PPNs and EPNs to achieve cost-effective and efficient IPM programs are presented. The common misuses of sampling and extraction methods are discussed. Professionals engaged in IPM should consider sampling the reliability level in the light of the intended goal, location, crop value, susceptibility, nematode species, and available funds. Logical sampling methodology should be expanded to integrate various factors that can recover extra EPN isolates with differential pathogenicity. It should seek for the best EPN-host matching. Merits of repeated baiting for EPN extraction from soil and sieving for PPN recovery from suspensions are presented. Their extraction values may be modelled to quantify the efficiency of nematode separation. The use of proper indices of dispersion to enhance the biocontrol potential of EPNs or save costs in nematicidal applications is ideally compatible with IPM programs. Selecting an extraction method may sometimes require further tests to find the best extraction method of the existing fauna and/or flora. Cons and pros of modern sampling and extraction techniques are highlighted.

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