scholarly journals Biocontrol potential of entomopathogenic nematode species against Tribolium confusum (Jac.) (Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Fab.) (Coleoptera: Bostrichidae) under laboratory conditions

Author(s):  
Salma Javed ◽  
Tabassum Ara Khanum ◽  
Samreen Khan
Nematology ◽  
2014 ◽  
Vol 16 (6) ◽  
pp. 729-737 ◽  
Author(s):  
Shervin Sharifi ◽  
Shervin Sharifi ◽  
Javad Karimi ◽  
Shervin Sharifi ◽  
Javad Karimi ◽  
...  

Rosaceae longhorned beetle, Osphranteria coerulescens, is an important pest of fruit trees that attacks all fruit trees belonging to the family Rosaceae in cold regions of Iran. The potential efficacy of two species of entomopathogenic nematodes (EPN), Heterorhabditis bacteriophora and Steinernema carpocapsae, against last instar larvae of this pest was tested under laboratory conditions. In plate assays, the larvae were susceptible to both EPN species but were more susceptible to S. carpocapsae (65.0-97.5% mortality) than H. bacteriophora (42.5-87.8%). Both nematode species were able to penetrate and reproduce within O. coerulescens larvae, but reproduction rate for H. bacteriophora was higher than those of S. carpocapsae; however, the penetration rate for S. carpocapsae was greater than H. bacteriophora. In a migration test on agar plate, S. carpocapsae showed negligible attraction to the pest or to Galleria mellonella cues. However, H. bacteriophora was strongly attracted to the sector of Petri dishes containing larvae. In a test using apricot tree branches, both species of EPN passed from mass frass of O. coerulescens larvae in the tip of the branches, penetrated into the larval galleries, and located and killed the larvae of O. coerulescens in their natural habitat deep inside the branches. Our findings provide the first insight into the biocontrol efficacy of EPN against O. coerulescens larvae, and highlight their potential for the control of this pest. Field experiments are needed to evaluate their potential under the environmental conditions in which rosaceae longhorned beetle larvae are found.


2020 ◽  
Vol 94 ◽  
Author(s):  
P.H. Mhatre ◽  
J. Patil ◽  
V. Rangasamy ◽  
K.L. Divya ◽  
S. Tadigiri ◽  
...  

Abstract The potato tuber moth, Phthorimaea operculella (Zeller), is a serious pest of potato and other commercial crops belonging to the Solanaceae family. In recent years, it has become an emerging problem in potato-growing regions of the Nilgiri hills of southern India. It is responsible for the reduced quality and quantity of marketable potatoes. In this regard, the development of an eco-friendly control method for the management of the potato tuber moth is urgently required. Therefore, in the present study, the virulence of Steinernema cholashanense CPRSUS01 originally isolated from the potato rhizosphere was tested on fourth-instar larvae and pupae of P. operculella. Steinernema cholashanense caused the greatest mortality in the fourth-instar larval stage (100%) than the pupae (30%). In addition to this, penetration and reproduction of this nematode was also studied in fourth-instar larvae of P. operculella and this is the first report of penetration and reproduction of any entomopathogenic nematode species on potato tuber moth larvae. The reproduction capacity of S. cholashanense on P. operculella is higher (702 infective juveniles mg−1 body weight). Our results indicated that S. cholashanense has good potential as an alternative tool for the management of P. operculella. But before including S. cholashanense in the integrated pest management program of P. operculella, its efficacy should be tested under field conditions.


Author(s):  
Şeyda Şimşek ◽  
Mehtap Gürsoy ◽  
Seher Karaman Erkul

In this study, the toxic effects of methanol extracts obtained from, Reseda lutea L. (Resedaceae), Salvia absconditiflora Greuter & Burdet. (Lamiaceae), Thymus leucostomus Hausskn. & Velen. (Lamiaceae), Chorispora purpurascens (Banks & Sol.) Eig (Brassicaceae), Isatis floribunda Boiss. ex Bornm. (Brassicaceae) and Linum tenuifolium L. (Linaceae) plants were tested on Tribolium confusum Duv. (Col.: Tenebrionidae) and Rhyzopertha dominica F. (Col.: Bostrichidae) in laboratory conditions. The methanol extracts obtained from the plants were applied to the adults of the pests by micro-aplicator. As a result of the single dose (10% (w/v)) effect study, the highest contact toxicity against T. confusum (23.17%) and R. dominica (18.35%) showed the extract obtained from C. purpurascens plant.


2010 ◽  
Vol 54 (3) ◽  
pp. 166-171 ◽  
Author(s):  
Z. Ipek Ekmen ◽  
Selcuk Hazir ◽  
Ibrahim Cakmak ◽  
Nurdan Ozer ◽  
Mehmet Karagoz ◽  
...  

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