Potential negative effects on biological control by Sancassania polyphyllae (Acari: Acaridae) on an entomopathogenic nematode species

2010 ◽  
Vol 54 (3) ◽  
pp. 166-171 ◽  
Author(s):  
Z. Ipek Ekmen ◽  
Selcuk Hazir ◽  
Ibrahim Cakmak ◽  
Nurdan Ozer ◽  
Mehmet Karagoz ◽  
...  
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematode ◽  
Nematode Species ◽  
Negative Effects

Virulence of two entomopathogenic nematode species, Steinernema sp. (strain PQ16) and Heterorhabditis indica (strain KT3987), to nymphs of the coffee cicada Dundubia nagarasingna

Nematology ◽  
2019 ◽  
Vol 21 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Chau N. Nguyen ◽  
Anh T. Do ◽  
Phap Q. Trinh ◽  
Phuc K. Hoang
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Nematode Species ◽  
Mortality Rates ◽  
Coffee Plantations ◽  
Heterorhabditis Indica ◽  
Inoculation Dose ◽  
Pot Trial

Summary The virulence and efficacy of two species of entomopathogenic nematodes, Steinernema sp. (strain PQ16) and Heterorhabditis indica (strain KT3987), against nymphs of the coffee cicada, Dundubia nagarasingna, was evaluated under laboratory and glasshouse conditions. The highest mortality rates of coffee cicada nymphs caused by these two nematode strains were 93.5 and 100%, respectively, at an inoculation dose of 600 infective juveniles (IJ) nymph−1. The virulence (LC50) was established as 137.5 IJ and 149.1 IJ for strains S-PQ16 and H-KT3987, respectively. The highest IJ yields of these nematode strains were 66 × 103 IJ (for S-PQ16) and 134.4 × 103 IJ (for H-KT3987) at a dose of 500 IJ nymph−1. The efficacies of the two nematode strains to coffee cicadas at treated dose of 60 × 103 IJ pot−1 were 84.4 and 88.9% after 30 days, higher than the efficacies at treated dose of 40 × 103 IJ pot−1. The number of IJ in 250 ml of soil at 10, 20 and 30 days after treatment, increased from 0.38 × 103 to 4.80 × 103 IJ in soil treated with a dose of 40 × 103 IJ and from 0.66 × 103 to 5.02 × 103 IJ in soil treated with a dose of 60 × 103 IJ (for S-PQ16). Similarly, for H-KT3987 the number of IJ increased from 0.43 × 103 to 8.99 × 103 IJ and from 0.62 × 103 to 9.64 × 103 IJ, at the respective doses. Based on results of a pot trial from glasshouse modelling, an IJ application dosage for biological control of coffee cicada nymphs in coffee plantations was proposed.

scholarly journals Screening of entomopathogenic nematodes for virulence against the invasive western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) in Europe

2005 ◽  
Vol 95 (5) ◽  
pp. 473-482 ◽  
Author(s):  
S. Toepfer ◽  
C. Gueldenzoph ◽  
R.-U. Ehlers ◽  
U. Kuhlmann
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematode ◽  
Larval Mortality ◽  
Nematode Species ◽  
Diabrotica Virgifera Virgifera ◽  
Western Corn Rootworm ◽  
Corn Rootworm ◽  
Diabrotica Virgifera ◽  
Biological Control Agents ◽  
Steinernema Glaseri

AbstractEntomopathogenic nematode species available in Europe were screened for their efficacy against both the root-feeding larvae and silk-feeding adults of the western corn rootworm, Diabrotica virgifera virgifera LeConte. Laboratory screening tests were aimed at the selection of candidate biological control agents for the management of this invasive alien pest in Europe. Steinernema glaseri, S. arenarium, S. abassi, S. bicornutum, S. feltiae, S. kraussei, S. carpocapsae and Heterorhabditis bacteriophora were studied to determine their virulence against third instar larvae and adults of D. v. virgifera in small-volume arenas (using nematode concentrations of 0.5, 0.8, 7.9 and 15.9 infective juveniles cm–2). All nematode species were able to invade and propagate in D. v. virgifera larvae, but adults were rarely infected. At concentrations of 7.9 and 15.9 cm–2, S. glaseri, S. arenarium, S. abassi and H. bacteriophora caused the highest larval mortality of up to 77%. Steinernema bicornutum, S. abassi, S. carpocapsae and H. bacteriophora appeared to have a high propagation level, producing 5970±779, 5595±811, 5341±1177 and 4039±1025 infective juveniles per larva, respectively. Steinernema glaseri, S. arenarium, S. feltiae, S. kraussei and H. bacteriophora were further screened at a concentration of 16.7 nematodes cm–2 against third instar larvae in medium-volume arenas (sand-filled trays with maize plants). Heterorhabditis bacteriophora, S. arenarium and S. feltiae caused the highest larval mortality with 77±16.6%, 67±3.5%, and 57±17.1%, respectively. In a next step, criteria for rating the entomopathogenic nematode species were applied based on results obtained for virulence and propagation, and for current production costs and availability in Europe. These criteria were then rated to determine the potential of the nematodes for further field testing. Results showed the highest potential in H. bacteriophora, followed by S. arenarium and S. feltiae, for further testing as candidate biological control agents.

scholarly journals Virulence of four entomopathogenic nematode against different stages of the Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Mürşide Yağcı ◽  
Tuğba Akdeniz Fırat ◽  
F. Dolunay Erdoğuş ◽  
Müge Şahin
Keyword(s):  
Biological Control ◽  
Ceratitis Capitata ◽  
Biological Control Agent ◽  
Entomopathogenic Nematode ◽  
Fruit Fly ◽  
Adult Mortality ◽  
Control Agent ◽  
Mediterranean Fruit Fly ◽  
Pest Species ◽  
Negative Effects

Abstract Background Ceratitis capitata Wiedemann (Diptera: Tephritidae) (Mediterranean fruit fly), is one of the important polyphagous pest species in the world that harms almost all fruits causing significant yields losses. Control of this pest which is on the quarantine list of many countries is highly important. Due to the negative effects of chemical control on the environment and human health, biological control approaches have gained importance. Entomopathogenic nematodes (EPNs) are a potential biological control agent that can be used for effectively controlling the Medfly. Results In this study, effects of four EPNs species on larvae, pupae and adults of Medfly were investigated under laboratory conditions. Four different concentrations of EPNs species were used against each stage of the pest. It pest showed different sensitivity to different concentrations of each of the tested EPNs. High mortality rate of 94% was caused by Heterorhabditis bacteriophora (11 KG),, followed by H. bacteriophora (TOK-20) with 91%, Steinernema carpocapsae (85%) and S. feltiae (Tokat-Emir) with 71% at highest concentration (200 IJ/larvae. The highest effect on adult mortality (100%) was recorded by the species S. feltiae (Tokat-Emir) at the highest concentration (200 IJ/adult) in the trials, followed by H. bacteriophora (11 KG) with 92%, H. bacteriophora (TOK-20) with 91% and S. carpocapsae (Tokat-Bakışlı05) with 87.37% mortality rates at the same concentrations. Conclusions It was determined that the EPNs tested in this study had insecticidal properties and they might be used in biological control programs against the Medfly.

Entomopathogenic nematodes: A tool in biocontrol of insect pests of vegetables-A review

2017 ◽  
Vol 38 (02) ◽  
Author(s):  
Gitanjali Devi ◽  
Dhrubajyoti Nath
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Insect Pest ◽  
Nematode Species ◽  
Vegetable Crops ◽  
Biological Control Agents ◽  
Isolation And Identification ◽  
Identification Technique

Biological control agents have become increasingly important component in integrated pest management programme. Entomopathogenic nematodes are effective biological control agents for many important insect pests of vegetable crops. Therefore entomopathogenic nematodes are gaining attention in the field of biocontrol research worldwide. With the development and improvement of isolation and identification technique many novel species and strains have been utilized for management of several insect pests. This review aims to explore the potentiality of entomopathogenic nematode species against economically important insect pest of vegetables in India as well as in other countries.

Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi

2015 ◽  
Vol 90 (6) ◽  
pp. 706-711 ◽  
Author(s):  
C.A. Saumell ◽  
A.S. Fernández ◽  
F. Echevarria ◽  
I. Gonçalves ◽  
L. Iglesias ◽  
...  
Keyword(s):  
Biological Control ◽  
Environmental Effects ◽  
Biological Control Agent ◽  
Control Agent ◽  
Nematophagous Fungi ◽  
Soil Nematodes ◽  
Duddingtonia Flagrans ◽  
Negative Effects ◽  
Post Deposition

AbstractThe possible environmental effects of the massive use of Duddingtonia flagrans for controlling sheep nematodes were evaluated in two regions. Non-supplemented faeces and faeces from sheep supplemented with D. flagrans were deposited three times on pasture plots and samples were collected 7 and 14 days post-deposition. Samples were cultured in agar-water (2%) with Panagrellus spp. to recover D. flagrans and other nematophagous fungi, and soil nematodes were extracted using Baermann funnels and counted. No significant differences in the populations of soil nematodes and fungi colonizing sheep faeces (P > 0.05) were observed between supplemented and non-supplemented groups, except in one sample. The topsoil in contact with the faeces was sampled 1–4 months post-deposition, revealing that, with one exception, D. flagrans did not persist in soil beyond 2 months post-deposition. Duddingtonia flagrans does not affect faecal colonization by other fungi and soil nematodes and, once deployed on pasture, does not survive for long periods in the environment.

scholarly journals Virulence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) for the control of Diabrotica speciosa germar (coleoptera: chrysomelidae)

2011 ◽  
Vol 35 (6) ◽  
pp. 1149-1156 ◽  
Author(s):  
Viviane Santos ◽  
Alcides Moino Junior ◽  
Vanessa Andaló ◽  
Camila Costa Moreira ◽  
Ricardo Alves de Olinda
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematodes ◽  
Entomopathogenic Nematode ◽  
Larval Mortality ◽  
Steinernema Glaseri ◽  
Soil Insects ◽  
Diabrotica Speciosa ◽  
Pupal Mortality

Entomopathogenic nematodes (EPNs) are used in biological control of soil insects and show promise in the control of D. speciosa. The objective of this work was to evaluate the potential of native and exotic entomopathogenic nematode isolates in the control of D. speciosa under laboratory and greenhouse conditions. Results showed that all of EPNs caused larval mortality. The most virulent were Heterorhabditis sp. RSC01 (94%), Steinernema glaseri (84%), Heterorhabditis sp. JPM04 (82%) and Heterorhabditis amazonensis RSC05 (78%). There was no effect of the Heterorhabditis sp. RSC01 and S. glaseri isolates on eggs. The maximum mortality of D. speciosa larvae by Heterorhabditis sp. RSC01 was observed at a concentration of 300 IJ/ insect, while by S. glaseri observed the highest mortality at the concentration of 200 IJ/ insect. The Heterorhabditis sp. RSC01 isolate caused over 80% pupal mortality at a concentration of 250 IJ/insect. The virulence of Heterorhabditis sp. RSC01 and S. glaseri was affected by temperature. The Heterorhabditis sp. RSC01 isolate caused reduction in larva survival under greenhouse conditions at all of the tested concentrations and there was no difference in mortality among different concentrations of infectid juveniles.

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