Abnormalities in Winter Flounder (Pleuronectes Americanus) Living Near a Paper Mill in the Humber Arm, Newfoundland

2020 ◽  
pp. 511-523
Author(s):  
R.A. Khan ◽  
D.E. Barker ◽  
K. Ryan ◽  
B. Murphy ◽  
R.G. Hooper
Keyword(s):  
Paper Mill ◽  
Winter Flounder ◽  
Pleuronectes Americanus

Bioindicators of Stress in Winter Flounder, Pleuronectes americanus, Captured Adjacent to a Pulp and Paper Mill in St. George's Bay, Newfoundland

1994 ◽  
Vol 51 (10) ◽  
pp. 2203-2209 ◽  
Author(s):  
D. E. Barker ◽  
R. A. Khan ◽  
R. Hooper
Keyword(s):  
Reference Site ◽  
Paper Mill ◽  
Pulp And Paper ◽  
Winter Flounder ◽  
Digenetic Trematode ◽  
Pleuronectes Americanus ◽  
Pulp And Paper Mill ◽  
High Incidence ◽  
Blood Haemoglobin ◽  
Anisakid Nematodes

Winter flounder, Pleuronectes americanus, inhabiting a marine inlet (Port Harmon) adjacent to a pulp and paper mill, and from a reference site (St. George's) 12 km away, were examined for signs of stress. Fish from Port Harmon showed significantly lower condition (k) factors, hepatosomatic indices, blood haemoglobin, and lymphocyte levels, than those from the reference site. Delayed spawning or spermiation were evident in female and male flounder, respectively, from Port Harmon compared with those from St. George's. Prevalence and intensity of larval anisakid nematodes, metacercariae of the digenetic trematode, Cryptocotyle lingua, and cysts of the microsporan, Glugea stephani, were significantly higher at Port Harmon than at St. George's, whereas prevalence and intensity of an intestinal acanthocephalan were significantly lower at Port Harmon. The percentage of flounder with external lesions (fin necrosis, ulcers) was significantly higher at Port Harmon than at St. George's. Anaemia, low lymphocyte levels, low somatic indices, high incidence of external lesions, and abnormal parasitic infestations are all suggestive of immunosuppression. Because basic water chemistry at the two sites was similar, the evidence of chronic stress observed in winter flounder from Port Harmon can most likely be attributed to pollutants from the effluent that have accumulated in the sediment.

Histopathology in winter flounder (Pleuronectes americanus) living adjacent to a pulp and paper mill

1994 ◽  
Vol 26 (1) ◽  
pp. 95-102 ◽  
Author(s):  
R.A. Khan ◽  
D.E. Barker ◽  
R. Hooper ◽  
E.M. Lee ◽  
K. Ryan ◽  
...  
Keyword(s):  
Paper Mill ◽  
Pulp And Paper ◽  
Winter Flounder ◽  
Pleuronectes Americanus ◽  
Pulp And Paper Mill

Induced Cytochrome P4501A in Winter Flounder, Pleuronectes americanus, from Offshore and Coastal Sites

1994 ◽  
Vol 51 (4) ◽  
pp. 933-941 ◽  
Author(s):  
Emily Monosson ◽  
John J. Stegeman
Keyword(s):  
East Coast ◽  
Dry Weight ◽  
Winter Flounder ◽  
Boston Harbor ◽  
Pleuronectes Americanus ◽  
Georges Bank ◽  
Urban Centers ◽  
Cytochrome P4501a ◽  
Passamaquoddy Bay ◽  
Specific Activities

Cytochrome P4501A (CYP1A), Aroclor 1254 (A1254), and 3,3′,4,4′-tetrachlorobiphenyl (TCB) were measured in liver of winter flounder, Pleuronectes americanus, from Boston Harbor, Mass., Hempsted Harbor, N.Y., Niantic, Conn., and an offshore site, Georges Bank. We also measured CYP1A content and activity in flounder from Passamaquoddy Bay, N.B. Concentrations of A1254 and TCB were the least in fish from Georges Bank (0.46 and 0.002 μg∙g dry weight−1, respectively); concentrations in fish from Boston, Niantic, and Hempsted ranged from 7.6 to 11.3 μg∙g−1 and from 0.013 to 0.024 μg∙g−1. Immunodetected microsomal CYP1A contents (expressed as scup P450E equivalents) were 0.17 and 0.19 nmol∙mg−1 in fish from Georges Bank and Passamaquoddy and 0.25–0.41 nmol∙mg−1 in fish from Boston, Niantic, and Hempsted. Ethoxyresorufin-O-deethylase specific activities likewise were greater in fish from Boston, Niantic, and Hempsted (1.7–2.4 nmol∙min−1∙mg−1) than in fish from Georges Bank or Passamaquoddy (0.83 and 0.61 nmol∙min−1∙mg−1). CYP1A content and activity were correlated with hepatic concentrations of A1254 and TCB. These data, together with data reported in previous studies, indicate that strong induction of CYP1A protein occurs in winter flounder populations along most of the industrialized east coast and that induction of CYP1A is common, but less strong, at sites distant from the urban centers of the Northeast.

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