In some industrial processes, and especially in agrofood industries, the cleaning in place mechanism used for hydraulic circuits plays an important role. This process needs a good knowledge of the hydrodynamic flows to determinate the appropriate parameters that assure a good cleaning of these circuits without disassembling them. Generally, different arrangements are present in these hydraulic circuits, such as expansions, diffusers, and elbows. The flow crossing these singularities strongly affects the process of cleaning in place. This work is then a contribution to complete recent studies of “aliments quality security” project to ameliorate the quality of the cleaning in place. It presents experimental and numerical investigations of a confined turbulent flow behavior across a conical diffuser (2α=16 deg). The role of a perturbation caused by the presence of an elbow in the test section, upstream of the progressive enlargement, was studied. The main measurements were the static pressure and the instantaneous velocity fields using the particle image velocimetry (PIV). Post-processing of these PIV measurements were adopted using the Γ2 criterion for the vortices detection and the proper orthogonal decomposition (POD) technique to extract the most energetic modes contained in the turbulent flow and to the turbulent flow filtering. A database has been also constituted and was used to test the validity of the most models of turbulence, and in particular, a variant of the shear stress transport (SST) model.