C677T Mutation in the Methylenetetrahydrofolate Reductase Gene Increases Serum Uric Acid in Obese Subjects.

2010 ◽  
pp. P1-448-P1-448
Author(s):  
A Kogure ◽  
T Harayama ◽  
Y Takakura ◽  
T Umekawa ◽  
K Yoshioka ◽  
...  
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Methylenetetrahydrofolate Reductase ◽  
Reductase Gene ◽  
C677t Mutation ◽  
Obese Subjects ◽  
Methylenetetrahydrofolate Reductase Gene

Methylenetetrahydrofolate reductase gene C677T mutation and plasma homocysteine level in Beh�et's disease

2003 ◽  
Vol 23 (5) ◽  
pp. 236-240 ◽  
Author(s):  
Abdullah Canataroglu ◽  
Kahraman Tanriverdi ◽  
Tamer Inal ◽  
Gulsah Seydaoglu ◽  
Didem Arslan ◽  
...  
Keyword(s):  
Methylenetetrahydrofolate Reductase ◽  
Homocysteine Level ◽  
Plasma Homocysteine ◽  
Plasma Homocysteine Level ◽  
Reductase Gene ◽  
C677t Mutation ◽  
Methylenetetrahydrofolate Reductase Gene

scholarly journals C677T mutation of methylenetetrahydrofolate reductase gene determined in blood or plasma by multiple-injection capillary electrophoresis and laser-induced fluorescence detection

1997 ◽  
Vol 43 (2) ◽  
pp. 267-272 ◽  
Author(s):  
Arve Ulvik ◽  
Helga Refsum ◽  
Leo A J Kluijtmans ◽  
Per Magne Ueland
Keyword(s):  
Capillary Electrophoresis ◽  
Fluorescence Detection ◽  
Methylenetetrahydrofolate Reductase ◽  
Laser Induced Fluorescence ◽  
Recognition Site ◽  
Multiple Injection ◽  
Laser Induced Fluorescence Detection ◽  
Reductase Gene ◽  
C677t Mutation ◽  
Methylenetetrahydrofolate Reductase Gene

Abstract We constructed an assay to detect the common C677T mutation in the methylenetetrahydrofolate reductase gene. The mutation creates a Hinfl recognition site detected by restriction cleavage of a 198-bp fragment amplified in the polymerase chain reaction (PCR). Digested samples were subjected to capillary electrophoresis with laser-induced fluorescence detection (CE-LIF), with hydroxypropylmethylcellulose as the sieving matrix and SYBR Green I as the fluorescent dye. After amplification but before digestion, we added to the PCR mixture a fragment with the HinfI recognition site and a 15-bp truncation at the 3′ end. Using this procedure, we could (a) verify completeness of digestion and monitor injection, (b) assign genotypes on the basis of pattern recognition, and (c) develop a multiple-injection mode with simultaneous separation of as many as eight samples. A seminested PCR protocol in combination with CE-LIF allowed genotyping of plasma/serum samples 20 years old.

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