LH Receptor Activation Regulates Adiponectin Receptor Expression in Human Granulosa Cells Via a cAMP Pathway.

2010 ◽  
pp. P2-384-P2-384
Author(s):  
EP Wickham ◽  
T Tao ◽  
KE Salley ◽  
JE Nestler ◽  
EA McGee
Keyword(s):  
Granulosa Cells ◽  
Receptor Activation ◽  
Receptor Expression ◽  
Adiponectin Receptor ◽  
Lh Receptor ◽  
Human Granulosa Cells ◽  
Camp Pathway

scholarly journals Activation of the LH receptor up regulates the type 2 adiponectin receptor in human granulosa cells

2013 ◽  
Vol 30 (7) ◽  
pp. 963-968 ◽  
Author(s):  
Edmond P. Wickham ◽  
Tao Tao ◽  
John E. Nestler ◽  
Elizabeth A. McGee
Keyword(s):  
Granulosa Cells ◽  
Adiponectin Receptor ◽  
Lh Receptor ◽  
Human Granulosa Cells

scholarly journals TNF-α Suppressed FSH-Induced LH Receptor Expression Through Transcriptional Regulation in Rat Granulosa Cells

Endocrinology ◽  
2015 ◽  
Vol 156 (9) ◽  
pp. 3192-3202 ◽  
Author(s):  
Kohshiro Nakao ◽  
Hiroshi Kishi ◽  
Fumiharu Imai ◽  
Hiroto Suwa ◽  
Takashi Hirakawa ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Granulosa Cells ◽  
Promoter Region ◽  
Ovarian Function ◽  
Receptor Expression ◽  
Luciferase Assay ◽  
Pelvic Cavity ◽  
Transcriptional Level ◽  
Lh Receptor ◽  
Tnf Α

Several inflammatory cytokines regulate ovarian function. TNF-α is produced in granulosa cells under physiological conditions and has a reciprocal action on follicle development. In contrast, in pelvic inflammatory diseases, TNF-α is excessively produced in the pelvic cavity and has an adverse effect on reproductive functions. The objective of this study was to elucidate the mechanism of action of TNF-α on the expression of LH receptor (LHR) in immature rat granulosa cells. TNF-α suppressed FSH-induced LHR mRNA and protein expression and was not associated with cAMP accumulation. By using a luciferase assay, the construct containing base pairs −1389 to −1 of the rat Lhcgr promoter revealed that TNF-α decreased FSH-induced promoter activity. In response to TNF-α, nuclear factor (NF)-κB p65 was translocated to the nucleus, and the suppressive effect of TNF-α on LHR mRNA expression was abrogated by an NF-κB inhibitor. In a chromatin immunoprecipitation assay, TNF-α induced the association of NF-κB p65 with the rat Lhcgr transcriptional promoter region. NF-κB p65 and histone deacetylase (HDAC) interact to mediate expression of several genes at a transcriptional level. HDAC activity is thought to induce tight connections within local chromatin structures and repress gene transcription. Furthermore, the TNF-α–induced suppression of LHR mRNA expression was blocked by an HDAC inhibitor. Taken together, these results suggest that the interaction of NF-κB p65 with HDAC in the promoter region of rat Lhcgr might be responsible for TNF-α action on the regulation of LHR.

scholarly journals Luteal granulosa cells from natural cycles are more capable of maintaining their viability, steroidogenic activity and LH receptor expression than those of stimulated IVF cycles

2018 ◽  
Vol 34 (2) ◽  
pp. 345-355 ◽  
Author(s):  
Gamze Bildik ◽  
Nazli Akin ◽  
Ayse Seyhan ◽  
Yashar Esmaeilian ◽  
Kayhan Yakin ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Receptor Expression ◽  
Lh Receptor ◽  
Natural Cycles

scholarly journals Introduction of a gonadotropin receptor expression plasmid into immortalized granulosa cells leads to reconstitution of hormone-dependent steroidogenesis.

1992 ◽  
Vol 119 (2) ◽  
pp. 439-450 ◽  
Author(s):  
B S Suh ◽  
R Sprengel ◽  
I Keren-Tal ◽  
S Himmelhoch ◽  
A Amsterdam
Keyword(s):  
Granulosa Cells ◽  
Receptor Expression ◽  
Marker Enzyme ◽  
Dependent Manner ◽  
Ras Oncogene ◽  
Coding Region ◽  
Freezing And Thawing ◽  
Expression Plasmid ◽  
Lh Receptor ◽  
Cytochrome P450scc

We have recently succeeded in immortalizing rat granulosa cells by co-transfection with SV-40 DNA and the Ha-ras oncogene. These cells lost their response to gonadotropins, but expressed the cytochrome P450scc mitochondrial system enzymes and produced progesterone and 20 alpha-hydroxy-4-pregnan-3-one (20 alpha-OH-P) upon cAMP stimulation (Suh, B. S., and A. Amsterdam. 1990. Endocrinology. 127:2489-2500; Hanukoglu, I., B. S. Suh, S. Himmelhoch, and A. Amsterdam. 1990. J. Cell Biol. 111:1973-1981). In an attempt to restore the steroidogenic response to gonadotropins in immortalized cells, lutropin/choriogonadotropin (LH/CG-R) receptor expression plasmid was prepared by introducing the complete coding region of LH receptor cDNA (McFarland, K. C., R. Sprengel, H. S. Phillips, M. Köhler, N. Rosemblit, K. Nikolics, D. L. Segaloff, and P. H. Seeburg. 1989. Science (Wash. DC). 245:494-499) into a SV-40 early promoter based eucaryotic expression vector. Granulosa cells from preovulatory follicles were transfected with this LH receptor expression plasmid, together with SV-40 DNA and the Ha-ras oncogene. Cell lines obtained after this triple transfection accumulated cAMP in a dose-dependent manner in response to hCG. Moreover, they produced progesterone and 20 alpha-OH-P upon hCG stimulation with an ED50 of 125 pM and 75 pM, respectively, which is within the physiological range. Concomitantly with hCG induced differentiation, inhibition of cell proliferation was evident following stimulation with hormone concentrations as low as 40 pM. The number of hCG receptor sites per cell after numerous passages and several freezing and thawing cycles was 1.9 x 10(4), they showed a Kd of 180 pM. Stimulation with hCG induced pronounced morphological and biochemical changes in these cells including formation of mitochondrial located adrenodoxin, a marker enzyme for enhanced steroidogenesis. These findings make possible the expression in immortalized granulosa cells, of selectively mutated receptor molecules which preserve their steroidogenic potential, thereby opening the way to analysis of structure-function relationships of the receptor molecule.

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