Characterization of the 1,25-(OH)2D3-induced inhibition of bone nodule formation in long-term cultures of fetal rat calvaria cells.

Endocrinology ◽  
1993 ◽  
Vol 132 (1) ◽  
pp. 61-66 ◽  
Author(s):  
H Ishida ◽  
C G Bellows ◽  
J E Aubin ◽  
J N Heersche
Keyword(s):  
Nodule Formation ◽  
Bone Nodule ◽  
Rat Calvaria ◽  
Fetal Rat ◽  
Bone Nodule Formation

scholarly journals Sequential Expression of Bone Matrix Proteins During Rat Calvaria Osteoblast Differentiation and Bone Nodule Formation In Vitro

1997 ◽  
Vol 45 (4) ◽  
pp. 493-503 ◽  
Author(s):  
Jean R. Nefussi ◽  
Gabriel Brami ◽  
Dominique Modrowski ◽  
Martine Obcuf ◽  
Nadine Forest
Keyword(s):  
Bone Matrix ◽  
Matrix Proteins ◽  
Osteoblastic Cells ◽  
Nodule Formation ◽  
Bone Nodule ◽  
Rat Calvaria ◽  
Bone Matrix Proteins ◽  
Mineralized Matrix ◽  
Bone Nodule Formation

We investigated the expression of osteocalcin (OC), bone sialoprotein (BSP), osteonectin (ON), and alkaline phosphatase (ALP) during cell differentiation and bone nodule formation by fetal rat calvaria cells, using immunofluorescent and immunogold techniques at light and electron microscopic levels. Six hours after plating all proteins were expressed in calvaria cells. However, expression was not detected during the proliferation phase after plating. Cell morphological modifications were observed in osteoblastic cells expressing ALP, OC, and BSP, but not ON. During the matrix formation phase, all proteins were expressed with various intensities and OC was limited to differentiated osteoblastic cells. EM observations demonstrated that BSP was selectively associated with clusters of needle-like crystals, but not with collagen fibers, in mineralization foci and in the mineralized matrix. OC was localized intracellularly and in all the extracellular compartments, and was concentrated at the mineralization front. ON was distributed uniformly throughout the osteoid and mineralized matrix, which was intensely labeled. The results show that the expression of bone matrix proteins during differentiation of calvaria cells and nodule formation in vitro duplicate what is observed during osteogenesis in vivo.

Interleukin-1α stimulates the release of prostaglandin E2 and phospholipase A2 from fetal rat calvarial cells in vitro: Relationship to bone nodule formation

2009 ◽  
Vol 6 (8) ◽  
pp. 843-850 ◽  
Author(s):  
Lesley G. Ellies ◽  
Johan N.M. Heersche ◽  
Peter Vadas ◽  
Waldemar Pruzanski ◽  
Eva Stefanski ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Phospholipase A2 ◽  
Nodule Formation ◽  
Bone Nodule ◽  
Fetal Rat ◽  
Interleukin 1Α ◽  
Bone Nodule Formation

Characterization of Osteoblast-like Cells from Fetal Rat Calvaria

1980 ◽  
Vol 59 (11) ◽  
pp. 2006-2013 ◽  
Author(s):  
B. Kadis ◽  
J.M. Goodson ◽  
S. Offenbacher ◽  
J.W. Bruns ◽  
S. Seibert
Keyword(s):  
Rat Calvaria ◽  
Fetal Rat

H2O2-derived free radicals treated fibronectin substratum reduces the bone nodule formation of rat calvarial osteoblast

1997 ◽  
Vol 98 (2) ◽  
pp. 113-125 ◽  
Author(s):  
Hiroshi Suzuki ◽  
Mitsuo Hayakawa ◽  
Kihei Kobayashi ◽  
Hisashi Takiguchi ◽  
Yoshimitsu Abiko
Keyword(s):  
Free Radicals ◽  
Nodule Formation ◽  
Bone Nodule ◽  
Calvarial Osteoblast ◽  
Bone Nodule Formation

Low-intensity laser irradiation stimulates bone nodule formation via insulin-like growth factor-I expression in rat calvarial cells

2007 ◽  
Vol 39 (6) ◽  
pp. 551-559 ◽  
Author(s):  
Noriyoshi Shimizu ◽  
Kotoe Mayahara ◽  
Takeshi Kiyosaki ◽  
Akikuni Yamaguchi ◽  
Yasuhiro Ozawa ◽  
...  
Keyword(s):  
Growth Factor ◽  
Laser Irradiation ◽  
Nodule Formation ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Low Intensity ◽  
Bone Nodule ◽  
Growth Factor I ◽  
Intensity Laser ◽  
Bone Nodule Formation
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