scholarly journals The Thyrotropin β-Subunit Gene Is Repressed by Thyroid Hormone in a Novel Thyrotrope Cell Line, Mouse TαT1 Cells**This work was supported by NIH Research Grants R01-HD-20377 and HD-12303 (to P.L.M.) and R01-DK-36843 (to E.C.R.); fellowships from the Spanish Ministry of Education and Science and Fundacion Jaime del Amo, Universidad Complutense de Madrid, Spain (to B.Y.); and a Ford Foundation Fellowship, the President’s Fellowship of the University of California, and NIH National Research Scientist Award Fellowship DK-09145 (to E.T.A.).

Endocrinology ◽  
1998 ◽  
Vol 139 (11) ◽  
pp. 4476-4482 ◽  
Author(s):  
Bernardo Yusta ◽  
Elaine T. Alarid ◽  
David F. Gordon ◽  
E. Chester Ridgway ◽  
Pamela L. Mellon
Keyword(s):  
Thyroid Hormone ◽  
Cell Line ◽  
Ford Foundation ◽  
University Of California ◽  
Β Subunit ◽  
Ministry Of Education ◽  
Subunit Gene ◽  
Research Grants ◽  
Research Scientist ◽  
The University

Thyroid Hormone Regulation of Thyrotropin α- and β-Subunit Gene Transcription

DNA ◽  
1985 ◽  
Vol 4 (4) ◽  
pp. 301-307 ◽  
Author(s):  
JAMES A. GURR ◽  
IONE A. KOURIDES
Keyword(s):  
Thyroid Hormone ◽  
Gene Transcription ◽  
Hormone Regulation ◽  
Β Subunit ◽  
Subunit Gene

The Mechanism of Negative Transcriptional Regulation by Thyroid Hormone: Lessons From the Thyrotropin β Subunit Gene

2018 ◽  
pp. 97-127 ◽  
Author(s):  
Shigekazu Sasaki ◽  
Akio Matsushita ◽  
Go Kuroda ◽  
Hiroko M. Nakamura ◽  
Yutaka Oki ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Thyroid Hormone ◽  
Β Subunit ◽  
Subunit Gene

The proto-oncogenes c-fos and c-jun modulate thyroid hormone inhibition of human thyrotropin β subunit gene expression in opposite directions

1992 ◽  
Vol 189 (2) ◽  
pp. 1050-1056 ◽  
Author(s):  
Donald L. Bodenner ◽  
John H. McClaskey ◽  
Myung K. Kim ◽  
A. James Mixson ◽  
Bruce D. Weintraub
Keyword(s):  
Gene Expression ◽  
Thyroid Hormone ◽  
Β Subunit ◽  
Subunit Gene

scholarly journals Activin and Glucocorticoids Synergistically Activate Follicle-Stimulating Hormone β-Subunit Gene Expression in the Immortalized LβT2 Gonadotrope Cell Line

Endocrinology ◽  
2007 ◽  
Vol 148 (2) ◽  
pp. 762-773 ◽  
Author(s):  
Shauna M. McGillivray ◽  
Varykina G. Thackray ◽  
Djurdjica Coss ◽  
Pamela L. Mellon
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Synergistic Interaction ◽  
Β Subunit ◽  
Subunit Gene ◽  
Rate Limiting Step ◽  
Reproductive Axis ◽  
Glucocorticoid Signaling ◽  
Tumor Virus

FSH is produced by the pituitary gonadotrope to regulate gametogenesis. Production of the β-subunit of FSH is the rate-limiting step in FSH synthesis, and a number of peptide and steroid hormones within the reproductive axis have been found to regulate transcription of the FSH β-subunit gene. Although both activin and glucocorticoids are notable regulators of FSHβ by themselves, we find that cotreatment results in a synergistic interaction on the mouse FSHβ promoter at the level of the gonadotrope using transient transfection of a reporter gene into the LβT2 immortalized gonadotrope-derived cell line. This synergistic interaction is specific to FSHβ, because only additive effects of these two hormones are observed on LH β-subunit, GnRH receptor, and mouse mammary tumor virus gene expression. Components of both activin and glucocorticoid signaling are found to be necessary for synergy, and there are specific cis elements on the mouse FSHβ promoter that contribute to the synergistic response as well. We also identify novel activin-responsive regions in the mouse FSHβ promoter and find that the −120 site can bind Smad2/3 in vitro. In addition, the glucocorticoid receptor and Smad3 are sufficient to confer a striking synergy with glucocorticoids on the mouse FSHβ promoter. Our studies provide the first evidence of a synergistic interaction between activin and glucocorticoids within the gonadotrope cell and demonstrate that this synergy can occur directly at the level of the mouse FSHβ promoter.

scholarly journals Thyroid Hormone Inhibition of Human Thyrotropin β-Subunit Gene Expression is Mediated by a Cis-acting Element Located in the First Exon

1989 ◽  
Vol 264 (25) ◽  
pp. 14601-14604 ◽  
Author(s):  
F E Wondisford ◽  
E A Farr ◽  
S Radovick ◽  
H J Steinfelder ◽  
J M Moates ◽  
...  
Keyword(s):  
Gene Expression ◽  
Thyroid Hormone ◽  
Β Subunit ◽  
Subunit Gene ◽  
Cis Acting

scholarly journals Thyroid Hormone Regulates the Mouse Thyrotropin β-Subunit Gene Promoter in Transfected Primary Thyrotropes

1989 ◽  
Vol 264 (25) ◽  
pp. 14840-14847 ◽  
Author(s):  
W M Wood ◽  
M Y Kao ◽  
D F Gordon ◽  
E C Ridgway
Keyword(s):  
Thyroid Hormone ◽  
Gene Promoter ◽  
Β Subunit ◽  
Subunit Gene
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