The Gly->Arg972 Amino Acid Polymorphism in Insulin Receptor Substrate-1 Affects Glucose Metabolism in Skeletal Muscle Cells

2000 ◽  
Vol 85 (5) ◽  
pp. 2004-2013 ◽  
Author(s):  
M. L. Hribal
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Glucose Metabolism ◽  
Insulin Receptor ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Insulin Receptor Substrate ◽  
Amino Acid Polymorphism ◽  
Insulin Receptor Substrate 1

scholarly journals Phosphorylation of Ser357of Rat Insulin Receptor Substrate-1 Mediates Adverse Effects of Protein Kinase C-δ on Insulin Action in Skeletal Muscle Cells

2008 ◽  
Vol 283 (17) ◽  
pp. 11226-11233 ◽  
Author(s):  
Rizwana Sanaullah Waraich ◽  
Cora Weigert ◽  
Hubert Kalbacher ◽  
Anita M. Hennige ◽  
Stefan Z. Lutz ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Adverse Effects ◽  
Insulin Receptor ◽  
Insulin Action ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Insulin Receptor Substrate ◽  
Kinase C

scholarly journals The Gly→Arg972Amino Acid Polymorphism in Insulin Receptor Substrate-1 Affects Glucose Metabolism in Skeletal Muscle Cells1

2000 ◽  
Vol 85 (5) ◽  
pp. 2004-2013
Author(s):  
Marta Letizia Hribal ◽  
Massimo Federici ◽  
Ottavia Porzio ◽  
Davide Lauro ◽  
Patrizia Borboni ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Metabolism ◽  
Insulin Receptor ◽  
Insulin Receptor Substrate ◽  
Insulin Receptor Substrate 1

scholarly journals Functional Studies of Akt Isoform Specificity in Skeletal Muscle in Vivo; Maintained Insulin Sensitivity Despite Reduced Insulin Receptor Substrate-1 Expression

2007 ◽  
Vol 21 (1) ◽  
pp. 215-228 ◽  
Author(s):  
Mark E. Cleasby ◽  
Tracie A. Reinten ◽  
Gregory J. Cooney ◽  
David E. James ◽  
Edward W. Kraegen
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Glucose Metabolism ◽  
Insulin Receptor ◽  
Glucose Uptake ◽  
Glycogen Synthase ◽  
Insulin Receptor Substrate ◽  
Insulin Receptor Substrate 1 ◽  
Short Hairpin

Abstract The phosphoinositide 3-kinase/Akt pathway is thought to be essential for normal insulin action and glucose metabolism in skeletal muscle and has been shown to be dysregulated in insulin resistance. However, the specific roles of and signaling pathways triggered by Akt isoforms have not been fully assessed in muscle in vivo. We overexpressed constitutively active (ca-) Akt-1 or Akt-2 constructs in muscle using in vivo electrotransfer and, after 1 wk, assessed the roles of each isoform on glucose metabolism and fiber growth. We achieved greater than 2.5-fold increases in total Ser473 phosphorylation in muscles expressing ca-Akt-1 and ca-Akt-2, respectively. Both isoforms caused hypertrophy of muscle fibers, consistent with increases in p70S6kinase phosphorylation, and a 60% increase in glycogen accumulation, although only Akt-1 increased glycogen synthase kinase-3β phosphorylation. Akt-2, but not Akt-1, increased basal glucose uptake (by 33%, P = 0.004) and incorporation into glycogen and lipids, suggesting a specific effect on glucose transport. Consistent with this, short hairpin RNA-mediated silencing of Akt-2 caused reductions in glycogen storage and glucose uptake. Consistent with Akt-mediated insulin receptor substrate 1 (IRS-1) degradation, we observed approximately 30% reductions in IRS-1 protein in muscle overexpressing ca-Akt-1 or ca-Akt-2. Despite this, we observed no decrease in insulin-stimulated glucose uptake. Furthermore, a 68% reduction in IRS-1 levels induced using short hairpin RNAs targeting IRS-1 also did not affect glucose disposal after a glucose load. These data indicate distinct roles for Akt-1 and Akt-2 in muscle glucose metabolism and that moderate reductions in IRS-1 expression do not result in the development of insulin resistance in skeletal muscle in vivo.

scholarly journals Differential Role of Insulin Receptor Substrate (IRS)-1 and IRS-2 in L6 Skeletal Muscle Cells Expressing the Arg1152→ Gln Insulin Receptor

1999 ◽  
Vol 274 (5) ◽  
pp. 3094-3102 ◽  
Author(s):  
Claudia Miele ◽  
Matilde Caruso ◽  
Veronique Calleja ◽  
Renata Auricchio ◽  
Francesco Oriente ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Receptor ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Insulin Receptor Substrate ◽  
L6 Skeletal Muscle Cells
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