Expression of the mRNA Coding for 11 -Hydroxysteroid Dehydrogenase Type 1 in Adipose Tissue from Obese Patients: An in Situ Hybridization Study

2002 ◽  
Vol 87 (6) ◽  
pp. 2701-2705 ◽  
Author(s):  
O. Paulmyer-Lacroix
Keyword(s):  
Adipose Tissue ◽  
In Situ Hybridization ◽  
Hydroxysteroid Dehydrogenase ◽  
Obese Patients ◽  
Hybridization Study

Overexpression of hepatic 5α-reductase and 11β-hydroxysteroid dehydrogenase type 1 in visceral adipose tissue is associated with hyperinsulinemia in morbidly obese patients

Metabolism ◽  
2011 ◽  
Vol 60 (12) ◽  
pp. 1775-1780 ◽  
Author(s):  
René Baudrand ◽  
José Miguel Domínguez ◽  
Cristian A. Carvajal ◽  
Arnoldo Riquelme ◽  
Carmen Campino ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Visceral Adipose

scholarly journals Localization of type 1 17beta-hydroxysteroid dehydrogenase mRNA and protein in syncytiotrophoblasts and invasive cytotrophoblasts in the human term villi

2000 ◽  
Vol 165 (2) ◽  
pp. 217-222 ◽  
Author(s):  
M Bonenfant ◽  
PR Provost ◽  
R Drolet ◽  
Y Tremblay
Keyword(s):  
In Situ Hybridization ◽  
Hydroxysteroid Dehydrogenase ◽  
Binding Activity ◽  
Placental Lactogen ◽  
Specific Expression ◽  
P450 Aromatase ◽  
Chain Cleavage ◽  
Extravillous Cytotrophoblasts

The 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) play a key role in the synthesis of sex steroids. The hallmark of this family of enzymes is the interconversion, through their oxydoreductive reactivity at position C17, of 17-keto- and 17beta-hydroxy-steroids. Because this reaction essentially transforms steroids having low binding activity for the steroid receptor to their more potent 17beta-hydroxysteroids isoforms, it is crucial to the control of the physiological activities of both estrogens and androgens. The human placenta produces large amounts of progesterone and estrogens throughout pregnancy. The placental type 1 17beta-HSD enzyme (E17beta-HSD) catalyzes the reduction of the low activity estrogen, estrone, into the potent estrogen, estradiol. We studied the cell-specific expression of type 1 17beta-HSD in human term placental villous tissue by combining in situ hybridization to localize type 1 17beta-HSD mRNA with immunohistochemistry using an antibody against human placental lactogen, a trophoblast marker. Immunolocalization of E17beta-HSD was also performed. To ascertain whether other steroidogenic enzymes are present in the same cell type, cytochrome P450 cholesterol side-chain cleavage (P450scc), P450 aromatase, and type 1 3beta-hydroxysteroid dehydrogenase (3beta-HSD) were also localized by immunostaining. Our results showed that the syncytium is the major steroidogenic unit of the fetal term villi. In fact, type 1 17beta-HSD mRNA and protein, as well as P450scc, P450 aromatase, and 3beta-HSD immunoreactivities were found in these cells. In addition, our results revealed undoubtedly that extravillous cytotrophoblasts (CTBs), e.g. those from which cell columns of anchoring villous originate, also express the type 1 17beta-HSD gene. However, CTBs lying beneath the syncytial layer, e.g. those from which syncytiotrophoblasts develop, contained barely detectable amounts of type 1 17beta-HSD mRNA as determined by in situ hybridization. These findings, along with those from other laboratories confirm the primordial role of the syncytium in the synthesis of steroids during pregnancy. In addition, our results indicate for the first time that CTBs differentiating along the invasive pathway contain type 1 17beta-HSD mRNA.

11β-Hydroxysteroid Dehydrogenase Type 1 mRNA is Increased in Both Visceral and Subcutaneous Adipose Tissue of Obese Patients*

Obesity ◽  
2006 ◽  
Vol 14 (5) ◽  
pp. 794-798 ◽  
Author(s):  
Raoul Desbriere ◽  
Vincent Vuaroqueaux ◽  
Vincent Achard ◽  
Sandrine Boullu-Ciocca ◽  
Martin Labuhn ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Obese Patients ◽  
Subcutaneous Adipose

11β-Hydroxysteroid Dehydrogenase Type 1 is Overexpressed in Subcutaneous Adipose Tissue of Morbidly Obese Patients

2008 ◽  
Vol 19 (6) ◽  
pp. 764-770 ◽  
Author(s):  
Rodrigo Muñoz ◽  
Cristian Carvajal ◽  
Alex Escalona ◽  
Camilo Boza ◽  
Gustavo Pérez ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Subcutaneous Adipose

scholarly journals Expression of the mRNA Coding for 11β-Hydroxysteroid Dehydrogenase Type 1 in Adipose Tissue from Obese Patients: Anin SituHybridization Study

2002 ◽  
Vol 87 (6) ◽  
pp. 2701-2705 ◽  
Author(s):  
Odile Paulmyer-Lacroix ◽  
Sandrine Boullu ◽  
Charles Oliver ◽  
Marie-Christine Alessi ◽  
Michel Grino
Keyword(s):  
Adipose Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Obese Patients

Overexpression of 11β-Hydroxysteroid Dehydrogenase Type 1 in Hepatic and Visceral Adipose Tissue is Associated with Metabolic Disorders in Morbidly Obese Patients

2009 ◽  
Vol 20 (1) ◽  
pp. 77-83 ◽  
Author(s):  
René Baudrand ◽  
Cristian A. Carvajal ◽  
Arnoldo Riquelme ◽  
Mauricio Morales ◽  
Nancy Solis ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Visceral Adipose Tissue ◽  
Metabolic Disorders ◽  
Hydroxysteroid Dehydrogenase ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Visceral Adipose

1003 In situ hybridization study of type 1 interleukin-1 receptor mRNA in the rat brain

1993 ◽  
Vol 18 ◽  
pp. S100
Author(s):  
Kazuki Yabuuchi ◽  
Masabumi Minami ◽  
Masamichi Satoh
Keyword(s):  
In Situ Hybridization ◽  
Rat Brain ◽  
Interleukin 1 ◽  
Hybridization Study ◽  
Receptor Mrna
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