Independent Dibutyryl Cyclic Adenosine Monophosphate Stimulation of Human Chorionic Gonadotropin and Estrogen Secretion by Malignant Trophoblast Cellsin Vitro

1974 ◽  
Vol 39 (5) ◽  
pp. 877-881 ◽  
Author(s):  
MICHAEL T. STORY ◽  
ROBERT O. HUSSA ◽  
ROLAND A. PATTILLO
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Malignant Trophoblast ◽  
Stimulation Of

scholarly journals Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus—oocyte complexes

2015 ◽  
Vol 63 (4) ◽  
pp. 485-498
Author(s):  
Tetsushi Ono ◽  
Asako Takaoka ◽  
Yasuhiro Morita ◽  
Lanh Thi Kim Do ◽  
Fuminori Tanihara ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Culture Medium ◽  
Collagen Gel ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Antral Follicle ◽  
The Mean ◽  
Almost All ◽  
Positive Effect

This study evaluated the effect of dibutyryl cyclic adenosine monophos-phate (dbcAMP) and human chorionic gonadotropin (hCG) on the formation of antral follicle-like structures (AFLSs) and on the meiotic status of bovine cumulus– oocyte complexes (COCs) embedded in collagen gel. Supplementation with dbcAMP increased the mean diameter of AFLSs during days 4–8 of culture compared with that of control COCs, irrespective of the concentration of dbcAMP used (0.5–2.0 mM). When the embedded COCs were cultured for 8 days with hCG, the diameters of AFLSs after 4 days of culture tended to be lower in the supplemented COCs than in the control COCs without hCG, irrespective of the concentration used (1–100 IU/mL). Supplementation with 10 IU/mL hCG increased the concentrations of anti-Müllerian hormone but not progesterone and oestradiol in the culture medium after 4 days of culture. Almost all oocytes collected from AFLSs had resumed meiosis by the end of culture, irrespective of supplementation of dbcAMP and hCG. These results indicate that although dbcAMP had a positive effect on AFLS formation and development, supplementation with hCG was detrimental. Moreover, hCG supplementation did not influence the luteinisation of granulosa cells in the AFLS for 4 days after the start of culture.

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