Waterjet Dissection of Peripheral Nerves: An Experimental Study of the Sciatic Nerve of Rats

2010 ◽  
Vol 67 (suppl_2) ◽  
pp. ons368-ons376 ◽  
Author(s):  
Christoph A. Tschan ◽  
Doerthe Keiner ◽  
Harald D. Müller ◽  
Kerstin Schwabe ◽  
Michael R. Gaab ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerves ◽  
Scar Tissue ◽  
Small Water ◽  
Peripheral Nerve Surgery ◽  
Sciatic Nerves ◽  
Nerve Surgery ◽  
Waterjet Dissection

ABSTRACT BACKGROUND: Although waterjet dissection has been well evaluated in intracranial pathologies, little is known of its qualities in peripheral nerve surgery. Theoretically, the precise dissection qualities could support the separation of nerves from adjacent tissues and improve the preservation of nerve integrity in peripheral nerve surgery. OBJECTIVE: To evaluate the potential of the new waterjet dissector in peripheral nerve surgery. METHODS: Waterjet dissection with pressures of 20 to 80 bar was applied on the sciatic nerves of 101 rats. The effect of waterjet dissection on the sciatic nerve was evaluated by clinical tests, neurophysiological examinations, and histopathological studies up to 12 weeks after surgery. RESULTS: With waterjet pressures up to 30 bar, the sciatic nerve was preserved in its integrity in all cases. Functional damaging was observed at pressures of 40 bar and higher. However, all but 1 rat in the 80 bar subgroup showed complete functional regeneration at 12 weeks after surgery. Histopathologically, small water bubbles were observed around the nerves. At 40 bar and higher, the sciatic nerves showed signs of direct nerve injury. However, all these animals showed nerve regeneration after 12 weeks, as demonstrated by histological studies. CONCLUSION: Sciatic nerves were preserved functionally and morphologically at pressures up to 30 bar. Between 40 and 80 bar, reliable functional and morphological nerve regeneration occurred. Waterjet pressures up to 30 bar might be applied safely under clinical conditions. This technique might be well suited to separate intact peripheral nerves from adjacent tumor or scar tissue. Further studies will have to show the clinical relevance of these dissection qualities.

scholarly journals E8002 Inhibits Peripheral Nerve Adhesion by Enhancing Fibrinolysis of l-Ascorbic Acid in a Rat Sciatic Nerve Model

2020 ◽  
Vol 21 (11) ◽  
pp. 3972
Author(s):  
Kiyoshi Kikuchi ◽  
Kentaro Setoyama ◽  
Seiya Takada ◽  
Shotaro Otsuka ◽  
Kazuki Nakanishi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
In Vitro Study ◽  
Scar Tissue ◽  
Tissue Type ◽  
Rat Sciatic Nerve ◽  
Peripheral Nerve Surgery ◽  
Nerve Surgery

Perineural adhesions leading to neuropathy are one of the most undesirable consequences of peripheral nerve surgery. However, there are currently no widely used compounds with anti-adhesive effects in the field of peripheral nerve surgery. E8002 is a novel, anti-adhesive, multi-layer membrane that contains L-ascorbic acid (AA). Here, we investigated the effect and mechanism of E8002 in a rat sciatic nerve adhesion model. A total of 21 rats were used. Six weeks after surgery, macroscopic adhesion scores were significantly lower in the E8002 group (adhesion procedure followed by nerve wrapping with E8002) compared to the E8002 AA(−) group (adhesion procedure followed by nerve wrapping with the E8002 membrane excluding AA) and adhesion group (adhesion procedure but no treatment). Correspondingly, a microscopic examination revealed prominent scar tissue in the E8002 AA(−) and adhesion groups. Furthermore, an in vitro study using human blood samples showed that AA enhanced tissue-type, plasminogen activator-mediated fibrinolysis. Altogether, these results suggest that E8002 may exert an anti-adhesive action via AA and the regulation of fibrinolysis.

Peripheral Retrograde Nerve Regeneration After Sciatic Nerve Injury in a Rat Model: A New Concept in Peripheral Nerve Surgery

2017 ◽  
Vol 04 (03) ◽  
Author(s):  
Zermeno Rivera ◽  
Jesus Jaime ◽  
Lopez Martinez Carlos Horacio ◽  
Fajardo Barajas Daniel ◽  
Gonzalez Jaime Jose de Jesus ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Rat Model ◽  
Sciatic Nerve Injury ◽  
Peripheral Nerve Surgery ◽  
Nerve Surgery

Reduction of Postoperative Perineural Adhesions by Hyaloglide Gel: An Experimental Study in the Rat Sciatic Nerve

2005 ◽  
Vol 56 (suppl_4) ◽  
pp. ONS-425-ONS-433 ◽  
Author(s):  
Phong Dam-Hieu ◽  
Catherine Lacroix ◽  
Gérard Said ◽  
Pauline Devanz ◽  
Song Liu ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Histological Analysis ◽  
Scar Tissue ◽  
High Viscosity ◽  
Contralateral Side ◽  
Adult Rats ◽  
Nerve Surgery

Abstract OBJECTIVE: To assess the effects of Hyaloglide gel (or auto-cross-linked polysaccharide [ACP] gel; Fidia Advanced Biopolymers, Abano Terme, Italy), a hyaluronan-derivative polymer, on peripheral nerve scarring and nerve regeneration. METHODS: We performed two surgical procedures in adult rats: 1) neurolysis of the sciatic nerve and separation of its tibial and peroneal branches, and 2) transection and immediate suture of the sciatic nerve. After nerve manipulation, ACP gel was applied onto the site of operation. We tested two solutions of ACP gel having different viscosities. Additional animals received Adcon-T/N (Gliatech, Inc., Cleveland, OH), an antiadhesive agent currently available for clinical use. No gel was applied on the contralateral side, which served as a control side. Four weeks later, the animals underwent reoperation. We assessed the quality of wound healing, the presence of perineural adherences, and the separability of nerves from surrounding tissues. RESULTS: Significantly fewer perineural adhesions were found in animals treated with ACP gel (high viscosity) and Adcon-T/N compared with controls. Quantitative histological analysis revealed a statistically significant reduction in the amount of scar tissue surrounding the nerves treated with ACP gel. No evidence of toxicity was found, and the gel did not interfere with nerve regeneration (counts of regenerating myelinated axons). CONCLUSION: ACP gel with high viscosity seems to be safe and effective in reducing perineural adhesions and scar formation after peripheral nerve surgery.

AN ECLECTIC REVIEW OF THE HISTORY OF PERIPHERAL NERVE SURGERY

Neurosurgery ◽  
2009 ◽  
Vol 65 (suppl_4) ◽  
pp. A3-A8 ◽  
Author(s):  
Allan H. Friedman
Keyword(s):  
Peripheral Nerve ◽  
Peripheral Nerves ◽  
Nerve Repair ◽  
Lessons Learned ◽  
Injured Nerve ◽  
Peripheral Nerve Surgery ◽  
Distinguished Career ◽  
History Of ◽  
Nerve Surgery

Abstract WE TAKE OUR present concepts of nerve repair for granted. In fact, the pioneers who established these principles traveled a road filled with erroneous dogma, bad advice, and misleading data. The lessons learned from a review of the history of peripheral nerve surgery are applicable to all neurosurgical disciplines. In honor of Dr. David Kline's distinguished career, we will review 3 aspects of the history of peripheral nerve surgery: Can an injured nerve regain function? How do peripheral nerves regenerate? When should a neuroma in continuity be resected?

Citicoline improves functional recovery, promotes nerve regeneration, and reduces postoperative scarring after peripheral nerve surgery in rats

2007 ◽  
Vol 68 (6) ◽  
pp. 615-622 ◽  
Author(s):  
Rafet Özay ◽  
Ahmet Bekar ◽  
Hasan Kocaeli ◽  
Necdet Karlı ◽  
Gülaydan Filiz ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Functional Recovery ◽  
Peripheral Nerve Surgery ◽  
Nerve Surgery

scholarly journals Pitfalls and problems encountered in rat model sciatic nerve surgery

2019 ◽  
pp. 396-399
Author(s):  
Marin Andrei ◽  
Mihai Ruxandra Ioana ◽  
Marin Georgiana Gabriela
Keyword(s):  
Sciatic Nerve ◽  
Surgical Technique ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Experimental Model ◽  
Rat Model ◽  
Peripheral Nerve Injury ◽  
Living Tissue ◽  
Nerve Surgery

When learning the basics of microsurgery, a trainee must be equipped with patience and perseverance in order to evolve. One must have the ground knowledge when it comes to peripheral nerve injury and nerve regeneration process in order to fully understand that the technique is vital for the outcome and final results. Furthermore, a trainee must practice on non-living tissue before performing successful in vivo operations and even in this case, one may be confronted with problems regarding the surgical technique. [1] The following article aims to reveal the main problems/mistakes when performing sciatic nerve surgery in an in vivo experimental model and the solutions for these problems.

scholarly journals Adipose Tissue Uses in Peripheral Nerve Surgery

2022 ◽  
Vol 23 (2) ◽  
pp. 644
Author(s):  
Allison Podsednik ◽  
Raysa Cabrejo ◽  
Joseph Rosen
Keyword(s):  
Peripheral Nerve ◽  
Peripheral Nerves ◽  
Animal Studies ◽  
Surgical Repair ◽  
Fat Grafting ◽  
Adipose Derived Stem Cells ◽  
Injury Site ◽  
Peripheral Nerve Surgery ◽  
Improved Outcomes ◽  
Nerve Surgery

Currently, many different techniques exist for the surgical repair of peripheral nerves. The degree of injury dictates the repair and, depending on the defect or injury of the peripheral nerve, plastic surgeons can perform nerve repairs, grafts, and transfers. All the previously listed techniques are routinely performed in human patients, but a novel addition to these peripheral nerve surgeries involves concomitant fat grafting to the repair site at the time of surgery. Fat grafting provides adipose-derived stem cells to the injury site. Though fat grafting is performed as an adjunct to some peripheral nerve surgeries, there is no clear evidence as to which procedures have improved outcomes resultant from concomitant fat grafting. This review explores the evidence presented in various animal studies regarding outcomes of fat grafting at the time of various types of peripheral nerve surgery.

Sign in / Sign up

Export Citation Format

Share Document