scholarly journals FGF stimulation of the Erk1/2 signalling cascade triggers transition of pluripotent embryonic stem cells from self-renewal to lineage commitment

Development ◽  
2007 ◽  
Vol 134 (16) ◽  
pp. 2895-2902 ◽  
Author(s):  
T. Kunath ◽  
M. K. Saba-El-Leil ◽  
M. Almousailleakh ◽  
J. Wray ◽  
S. Meloche ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Self Renewal ◽  
Signalling Cascade ◽  
Stimulation Of

scholarly journals Self-Renewal Versus Lineage Commitment of Embryonic Stem Cells: Protein Kinase C Signaling Shifts the Balance

Stem Cells ◽  
2011 ◽  
Vol 29 (4) ◽  
pp. 618-628 ◽  
Author(s):  
Debasree Dutta ◽  
Soma Ray ◽  
Pratik Home ◽  
Melissa Larson ◽  
Michael W. Wolfe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Self Renewal ◽  
Kinase C

scholarly journals ERK2 Suppresses Self-Renewal Capacity of Embryonic Stem Cells, but Is Not Required for Multi-Lineage Commitment

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e60907 ◽  
Author(s):  
William B. Hamilton ◽  
Keisuke Kaji ◽  
Tilo Kunath
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Self Renewal

scholarly journals C3G Regulates STAT3, ERK, Adhesion Signaling, and Is Essential for Differentiation of Embryonic Stem Cells

2021 ◽  
Author(s):  
Vijay V. Vishnu ◽  
Bh. Muralikrishna ◽  
Archana Verma ◽  
Sanjeev Chavan Nayak ◽  
Divya Tej Sowpati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Es Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Embryoid Bodies ◽  
Lineage Commitment ◽  
Self Renewal ◽  
Erk Activity

SummaryC3G (RAPGEF1), engaged in multiple signaling pathways, is essential for the early development of the mouse. In this study, we have examined its role in mouse embryonic stem cell self-renewal and differentiation. C3G null cells generated by CRISPR mediated knock-in of a targeting vector exhibited enhanced clonogenicity and long-term self-renewal. They did not differentiate in response to LIF withdrawal when compared to the wild type ES cells and were defective for lineage commitment upon teratoma formation in vivo. Gene expression analysis of C3G KO cells showed misregulated expression of a large number of genes compared with WT cells. They express higher levels of self-renewal factors like KLF4 and ESRRB and show high STAT3 activity, and very low ERK activity compared to WT cells. Reintroduction of C3G expression in a KO line partially reverted expression of ESRRB, and KLF4, and ERK activity similar to that seen in WT cells. The expression of self-renewal factors was persistent for a longer time, and induction of lineage-specific markers was not seen when C3G KO cells were induced to form embryoid bodies. C3G KO cells showed poor adhesion and significantly reduced levels of pFAK, pPaxillin, and Integrin-β1, in addition to downregulation of the cluster of genes involved in cell adhesion, compared to WT cells. Our results show that C3G is essential for the regulation of STAT3, ERK, and adhesion signaling, to maintain pluripotency of mouse embryonic stem cells and enable their lineage commitment for differentiation. Graphical abstract

scholarly journals Effects of Ionizing Radiation on Self-Renewal and Pluripotency of Human Embryonic Stem Cells

2010 ◽  
Vol 70 (13) ◽  
pp. 5539-5548 ◽  
Author(s):  
Kitchener D. Wilson ◽  
Ning Sun ◽  
Mei Huang ◽  
Wendy Y. Zhang ◽  
Andrew S. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Ionizing Radiation ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

Maintenance of Murine Embryonic Stem Cells' Self-Renewal and Pluripotency with Increase in Proliferation Rate by a Bovine Granulosa Cell Line-Conditioned Medium

2011 ◽  
Vol 20 (8) ◽  
pp. 1439-1449 ◽  
Author(s):  
Noelia Losino ◽  
Carlos Luzzani ◽  
Claudia Solari ◽  
Juan Boffi ◽  
Mariana Louis Tisserand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Line ◽  
Conditioned Medium ◽  
Granulosa Cell ◽  
Embryonic Stem ◽  
Proliferation Rate ◽  
Self Renewal ◽  
Murine Embryonic Stem Cells

scholarly journals Musashi2 Is Required for the Self-Renewal and Pluripotency of Embryonic Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34827 ◽  
Author(s):  
Erin L. Wuebben ◽  
Sunil K. Mallanna ◽  
Jesse L. Cox ◽  
Angie Rizzino
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
The Self ◽  
Self Renewal

scholarly journals Fam60a defines a variant Sin3a‐Hdac complex in embryonic stem cells required for self‐renewal

2017 ◽  
Vol 36 (15) ◽  
pp. 2216-2232 ◽  
Author(s):  
Gundula Streubel ◽  
Darren J Fitzpatrick ◽  
Giorgio Oliviero ◽  
Andrea Scelfo ◽  
Bruce Moran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals Environmental Oxygen Tension Regulates the Energy Metabolism and Self-Renewal of Human Embryonic Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62507 ◽  
Author(s):  
Catherine E. Forristal ◽  
David R. Christensen ◽  
Fay E. Chinnery ◽  
Raffaella Petruzzelli ◽  
Kate L. Parry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Energy Metabolism ◽  
Oxygen Tension ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals RNA Polymerase II-Association Factor 1 (Paf1/PD2) Maintains Self-renewal by Its Interaction with Oct3/4 in Mouse Embryonic Stem Cells

Stem Cells ◽  
2009 ◽  
pp. N/A-N/A ◽  
Author(s):  
Moorthy P. Ponnusamy ◽  
Shonali Deb ◽  
Parama Dey ◽  
Subhankar Chakraborty ◽  
Satyanarayana Rachagani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Self Renewal
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