An RNA-binding protein from Xenopus oocytes is associated with specific message sequences

Development ◽  
1987 ◽  
Vol 101 (4) ◽  
pp. 741-749 ◽  
Author(s):  
D.R. Crawford ◽  
J.D. Richter
Keyword(s):  
Xenopus Oocytes ◽  
Sucrose Gradient ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Cdna Libraries ◽  
Cdna Clones ◽  
Stage Of Development ◽  
Messenger Ribonucleoprotein ◽  
Stage 1

Monoclonal antibodies directed against an RNA-binding protein from Xenopus oocytes were used to immunoselect messenger ribonucleoprotein (mRNP) particles. RNA was extracted from both the immunoselected and nonselected fractions and was used to direct the synthesis of oligo (dT)-primed 32P-cDNA. These two cDNA preparations were then used to probe Xenopus stage-1 oocyte cDNA libraries to identify sequences that had been specifically coimmunoselected by the antibodies. Three cDNA clones were shown to be derived specifically from the antibody-selected mRNPs. During very early oogenesis (stage 1–2), the RNA-binding protein and the three coselected mRNAs sediment in the nontranslating mRNP region of a sucrose gradient. By oocyte stage 6, the binding protein concentration decreases by as much as 22-fold relative to polyadenylated RNA. At this stage of development, the three mRNAs are found predominantly in the polysome region of a sucrose gradient. These data demonstrate that Xenopus oocytes contain an RNA-binding protein which binds specific message sequences and may regulate their expression.

scholarly journals Vg1 RNA binding protein mediates the association of Vg1 RNA with microtubules in Xenopus oocytes.

1995 ◽  
Vol 14 (20) ◽  
pp. 5109-5114 ◽  
Author(s):  
Z. Elisha ◽  
L. Havin ◽  
I. Ringel ◽  
J. K. Yisraeli
Keyword(s):  
Xenopus Oocytes ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein

scholarly journals A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs.

1992 ◽  
Vol 89 (24) ◽  
pp. 11895-11899 ◽  
Author(s):  
S. P. Schwartz ◽  
L. Aisenthal ◽  
Z. Elisha ◽  
F. Oberman ◽  
J. K. Yisraeli
Keyword(s):  
Xenopus Oocytes ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Common Motif ◽  
Maternal Mrnas

Developmental characterization of a Drosophila RNA-binding protein homologous to the human systemic lupus erythematosus-associated La/SS-B autoantigen

1994 ◽  
Vol 14 (8) ◽  
pp. 5123-5129
Author(s):  
C Bai ◽  
Z Li ◽  
P P Tolias
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Polymerase Iii ◽  
Rna Binding Protein ◽  
Cdna Clones ◽  
Systemic Lupus ◽  
General Role ◽  
Visceral Mesoderm

Patients with humoral autoimmune diseases such as systemic lupus erythematosus and Sjögren's syndrome contain antibodies in their sera directed against certain normal cellular components such as the La/SS-B autoantigen, an RNA-binding protein believed to function as a putative processor of RNA polymerase III precursor transcripts. We have identified cDNA clones from the fruit fly Drosophila melanogaster that encode a protein displaying significant sequence homology with human La/SS-B. The fly protein (which we refer to as D-La) contains a putative ribonucleoprotein 1 (RNP1) and RNP2 RNA-binding domain. D-La also possesses a leucine zipper motif, suggesting that it may interact with itself or other proteins. Using gel retardation analysis, we show that D-La can bind RNA; in addition, we demonstrate the first reported DNA-binding activity associated with a La protein. Northern (RNA) blot analysis revealed a single 1,600-nucleotide transcript expressed throughout embryonic, larval, pupal, and adult development. Surprisingly, whole-mount in situ hybridization experiments revealed that D-La transcripts are not present in all ovarian tissues. In addition, early expression throughout the embryo is followed by a restricted pattern of mesodermal expression that is later confined to the visceral mesoderm, gonads, gut, and salivary glands. These results suggest that D-La may play a more specialized role during fly development as opposed to a rather general role inferred by its homology to La proteins from other organisms.

scholarly journals Developmental characterization of a Drosophila RNA-binding protein homologous to the human systemic lupus erythematosus-associated La/SS-B autoantigen.

1994 ◽  
Vol 14 (8) ◽  
pp. 5123-5129 ◽  
Author(s):  
C Bai ◽  
Z Li ◽  
P P Tolias
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Polymerase Iii ◽  
Rna Binding Protein ◽  
Cdna Clones ◽  
Systemic Lupus ◽  
General Role ◽  
Visceral Mesoderm

Patients with humoral autoimmune diseases such as systemic lupus erythematosus and Sjögren's syndrome contain antibodies in their sera directed against certain normal cellular components such as the La/SS-B autoantigen, an RNA-binding protein believed to function as a putative processor of RNA polymerase III precursor transcripts. We have identified cDNA clones from the fruit fly Drosophila melanogaster that encode a protein displaying significant sequence homology with human La/SS-B. The fly protein (which we refer to as D-La) contains a putative ribonucleoprotein 1 (RNP1) and RNP2 RNA-binding domain. D-La also possesses a leucine zipper motif, suggesting that it may interact with itself or other proteins. Using gel retardation analysis, we show that D-La can bind RNA; in addition, we demonstrate the first reported DNA-binding activity associated with a La protein. Northern (RNA) blot analysis revealed a single 1,600-nucleotide transcript expressed throughout embryonic, larval, pupal, and adult development. Surprisingly, whole-mount in situ hybridization experiments revealed that D-La transcripts are not present in all ovarian tissues. In addition, early expression throughout the embryo is followed by a restricted pattern of mesodermal expression that is later confined to the visceral mesoderm, gonads, gut, and salivary glands. These results suggest that D-La may play a more specialized role during fly development as opposed to a rather general role inferred by its homology to La proteins from other organisms.

235: The RNA-Binding Protein IMP3: A Novel Molecular Marker Predicts Progression of Superficial (TA and T1) Urothelial Carcinomas of Bladder

2007 ◽  
Vol 177 (4S) ◽  
pp. 78-79
Author(s):  
Lioudmila Sitnikova ◽  
Gary Mendese ◽  
Qin Lui ◽  
Bruce A. Woda ◽  
Di Lu ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Urothelial Carcinomas
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