Permissiveness to murine leukemia, virus expression during preimplantation and early postimplantation mouse development

Permissiveness to Moloney Murine Leukemia Virus (MoMuLV) expression was examined during preimplantation and early postimplantation development of the mouse embryo. Blastocysts and 8th, 9th and 10th day postimplantation embryos were infected in vitro with a MoMuLV-based retroviral vector expressing the lacZ gene driven off an internal rat beta-actin promoter. Beta-galactosidase-positive cells were identified in all embryonic tissues including inner cell mass, epiblast, mesoderm, endoderm and definitive ectoderm. In contrast, embryos infected with a MoMuLV-based vector expressing the lacZ gene driven off the viral LTR showed beta-galactosidase-positive cells only in mesoderm and definitive ectoderm. We conclude that permissiveness to transcriptional activity of the LTR is acquired immediately upon differentiation of epiblast during gastrulation of the mouse embryo.

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Sequential activation of the three protomers in the Moloney murine leukemia virus Env

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1617264114 ◽

2017 ◽

Vol 114 (10) ◽

pp. 2723-2728 ◽

Cited By ~ 3

Author(s):

Mathilda Sjöberg ◽

Robin Löving ◽

Birgitta Lindqvist ◽

Henrik Garoff

Keyword(s):

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Virus Envelope ◽

Sequential Activation ◽

Virus Envelope Protein ◽

Membrane Fusion Proteins ◽

Viral Membrane Fusion ◽

Murine Leukemia

Viral membrane fusion proteins of class I are trimers in which the protomeric unit is a complex of a surface subunit (SU) and a fusion active transmembrane subunit (TM). Here we have studied how the protomeric units of Moloney murine leukemia virus envelope protein (Env) are activated in relation to each other, sequentially or simultaneously. We followed the isomerization of the SU-TM disulfide and subsequent SU release from Env with biochemical methods and found that this early activation step occurred sequentially in the three protomers, generating two asymmetric oligomer intermediates according to the scheme (SU-TM)3→ (SU-TM)2TM → (SU-TM)TM2→ TM3. This was the case both when activation was triggered in vitro by depleting stabilizing Ca2+from solubilized Env and when viral Env was receptor triggered on rat XC cells. In the latter case, the activation reaction was too fast for direct observation of the intermediates, but they could be caught by alkylation of the isomerization active thiol.

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Heterogeneity of Murine Leukemia Virus in vitro DNA; Detection of Viral DNA in Mammalian Cells

Science ◽

10.1126/science.172.3990.1353 ◽

1971 ◽

Vol 172 (3990) ◽

pp. 1353-1355 ◽

Cited By ~ 55

Author(s):

L. D. Gelb ◽

S. A. Aaronson ◽

M. A. Martin

Keyword(s):

Murine Leukemia Virus ◽

Mammalian Cells ◽

Dna Detection ◽

Leukemia Virus ◽

Viral Dna ◽

Murine Leukemia

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Strong selection for cells containing new ecotropic recombinant murine leukemia virus provirus after propagation of C57BL/6 radiation-induced thymoma cells in vitro or in vivo

Molecular and Cellular Biology ◽

10.1128/mcb.3.9.1675-1679.1983 ◽

1983 ◽

Vol 3 (9) ◽

pp. 1675-1679

Author(s):

P Jolicoeur ◽

E Rassart ◽

P Sankar-Mistry

Keyword(s):

Cell Lines ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Primary Radiation ◽

Secondary Tumors ◽

Radiation Induced ◽

Viral Sequences ◽

Murine Leukemia

Using the Southern procedure, we have studied the presence of ecotropic-specific murine leukemia viral sequences in genomic DNA isolated from primary X-ray-induced thymomas, from lymphoid cell lines established from them, or from secondary tumors passaged in vivo. We found that primary radiation-induced thymomas and infiltrated spleens do not harbor newly acquired ecotropic provirus. However, additional ecotropic proviruses (which appear recombinant in the gagpol region) could be detected in most of the tumorigenic cell lines established in vitro from them and in tumors arising from subcutaneous transplantation of the primary thymomas. These results suggest that primary radiation-induced thymomas may not be clonal. They also indicate a strong correlation between the presence of ecotropic recombinant proviruses in the genome and the growth ability, both in vitro and in vivo, of specific cells within these thymomas, suggesting a possible mitogenic function for murine leukemia virus.

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IN VITRO LYMPHOID CELL TRANSFORMATION BY ABELSON MURINE LEUKEMIA VIRUS

Animal Virology ◽

10.1016/b978-0-12-077350-3.50023-x ◽

1976 ◽

pp. 311-320 ◽

Cited By ~ 1

Author(s):

Naomi Rosenberg ◽

David Baltimore

Keyword(s):

Lymphoid Cell ◽

Murine Leukemia Virus ◽

Cell Transformation ◽

Leukemia Virus ◽

Abelson Murine Leukemia Virus ◽

Murine Leukemia

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Inhibition of Gross Murine Leukemia Virus Replication by Rifamycin SV and Certain of Its Derivatives in vitro

Intervirology ◽

10.1159/000149744 ◽

1974 ◽

Vol 3 (1-2) ◽

pp. 84-96 ◽

Cited By ~ 6

Author(s):

William M. Shannon ◽

Louise Westbrook ◽

Frank M. Schabel, jr.

Keyword(s):

Virus Replication ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Rifamycin Sv ◽

Murine Leukemia

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Early Embryonic Lethality of Mice Lacking the Essential Protein SNEV

Molecular and Cellular Biology ◽

10.1128/mcb.01188-06 ◽

2007 ◽

Vol 27 (8) ◽

pp. 3123-3130 ◽

Cited By ~ 26

Author(s):

Klaus Fortschegger ◽

Bettina Wagner ◽

Regina Voglauer ◽

Hermann Katinger ◽

Maria Sibilia ◽

...

Keyword(s):

Matrix Protein ◽

Replicative Senescence ◽

Inner Cell Mass ◽

Umbilical Vein ◽

Cell Mass ◽

Preimplantation Embryos ◽

Proliferative Potential ◽

Mouse Development

ABSTRACT SNEV (Prp19, Pso4, NMP200) is a nuclear matrix protein known to be involved in pre-mRNA splicing, ubiquitylation, and DNA repair. In human umbilical vein endothelial cells, SNEV overexpression delayed the onset of replicative senescence. Here we analyzed the function of the mouse SNEV gene in vivo by employing homologous recombination in mice and conclude that SNEV is indispensable for early mouse development. Mutant preimplantation embryos initiated blastocyst formation but died shortly thereafter. Outgrowth of SNEV-null blastocysts showed a lack of proliferation of cells of the inner cell mass, which subsequently underwent cell death. While SNEV-heterozygous mice showed no overt phenotype, heterozygous mouse embryonic fibroblast cell lines with reduced SNEV levels displayed a decreased proliferative potential in vitro. Our experiments demonstrate that the SNEV protein is essential, functionally nonredundant, and indispensable for mouse development.

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