TGF beta 1 inhibits proliferation and has a chemotropic effect on mouse primordial germ cells in culture

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1451-1457 ◽  
Author(s):  
I. Godin ◽  
C.C. Wylie
Keyword(s):  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Target Tissue ◽  
Tgf Beta ◽  
Soluble Factors ◽  
Related Molecule ◽  
Feeder Layers ◽  
Stem Cell Populations ◽  
Embryonic Fibroblast

Primordial germ cells are the stem cells that provide the functional gametes of adult animals. In many animal groups they are set aside at the earliest stages of development, and migrate from their sites of first appearance to the sites where the gonad will form, the genital ridges. During this migration they proliferate. In the mouse embryo their numbers increase from less than one hundred to approximately four thousand during the period of their migration. In a previous paper we showed that both the proliferation and the direction of migration of mouse PGCs in culture were influenced by soluble factors released from their target tissue, the genital ridges. Studies on other stem cell populations have shown that complex combinations of growth factors control their proliferation, migration and differentiation. In this paper, we show that TGF beta 1 inhibits proliferation of PGCs taken from 8.5 day old embryos and cultured on embryonic fibroblast feeder layers. We also show that the previously reported chemotropic effect of genital ridges in this culture system is mediated by TGF beta 1, or a closely related molecule, released from the genital ridges.

Response to fibronectin of mouse primordial germ cells before, during and after migration

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1365-1373 ◽  
Author(s):  
C. Ffrench-Constant ◽  
A. Hollingsworth ◽  
J. Heasman ◽  
C.C. Wylie
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Explant Culture ◽  
Double Labelling ◽  
Target Tissue ◽  
Fibronectin Mrna

The adhesive extracellular matrix glycoprotein fibronectin is thought to play a central role in cell migration during embryogenesis. In order to define this role, we have examined the response to fibronectin in cell culture of mouse primordial germ cells (PGCs) before, during and after their migration from the hindgut into their target tissue, the genital ridges. Using an explant culture system, we show that PGCs will emigrate from tissue fragments containing hindgut, and that fibronectin stimulates this migration. Adhesion assays show that the start of PGC migration is associated with a fall in adhesion to fibronectin. Double-labelling studies using in situ hybridization and histochemistry demonstrate that migrating PGCs do not contain detectable fibronectin mRNA, suggesting that they do not synthesize and secrete the fibronectin within their migratory substratum. Taken together, these findings are consistent with an important role for fibronectin in stimulating PGC migration. In addition, however, they suggest that the interaction between PGCs and fibronectin may be important in timing the start of migration, with the fall in adhesion allowing the PGCs to commence their migration towards the genital ridges.

Collagen-alginate microspheres as a 3D culture system for mouse embryonic stem cells differentiation to primordial germ cells

Biologicals ◽  
2017 ◽  
Vol 48 ◽  
pp. 114-120 ◽  
Author(s):  
Vahid Mansouri ◽  
Mohammad Salehi ◽  
Mir davood Omrani ◽  
Zahra Niknam ◽  
Abdolreza Ardeshirylajimi
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
3D Culture ◽  
Mouse Embryonic Stem Cells ◽  
3D Culture System

Is the embryo culture system useful for collecting primordial germ cells from endangered avian embryos?

Zoo Biology ◽  
2002 ◽  
Vol 21 (3) ◽  
pp. 287-294 ◽  
Author(s):  
Takaharu Kawashima ◽  
Rika Sakai ◽  
Koichiro Kano ◽  
Yoshinori Tamaki ◽  
Koichiro Hashimoto
Keyword(s):  
Germ Cells ◽  
Embryo Culture ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Avian Embryos

scholarly journals Complete in vitro generation of fertile oocytes from mouse primordial germ cells

2016 ◽  
Vol 113 (32) ◽  
pp. 9021-9026 ◽  
Author(s):  
Kanako Morohaku ◽  
Ren Tanimoto ◽  
Keisuke Sasaki ◽  
Ryouka Kawahara-Miki ◽  
Tomohiro Kono ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Regenerative Medicine ◽  
Genomic Imprinting ◽  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Oocyte Growth ◽  
In Vitro System ◽  
In Vitro Reconstitution

Reconstituting gametogenesis in vitro is a key goal for reproductive biology and regenerative medicine. Successful in vitro reconstitution of primordial germ cells and spermatogenesis has recently had a significant effect in the field. However, recapitulation of oogenesis in vitro remains unachieved. Here we demonstrate the first reconstitution, to our knowledge, of the entire process of mammalian oogenesis in vitro from primordial germ cells, using an estrogen-receptor antagonist that promotes normal follicle formation, which in turn is crucial for supporting oocyte growth. The fundamental events in oogenesis (i.e., meiosis, oocyte growth, and genomic imprinting) were reproduced in the culture system. The most rigorous evidence of the recapitulation of oogenesis was the birth of fertile offspring, with a maximum of seven pups obtained from a cultured gonad. Moreover, cryopreserved gonads yielded functional oocytes and offspring in this culture system. Thus, our in vitro system will enable both innovative approaches for a deeper understanding of oogenesis and a new avenue to create and preserve female germ cells.

Derivation of chicken primordial germ cells using an indirect Co-culture system

2019 ◽  
Vol 123 ◽  
pp. 83-89 ◽  
Author(s):  
Long Xie ◽  
Zhenping Lu ◽  
Dongyang Chen ◽  
Mengmeng Yang ◽  
Yuying Liao ◽  
...  
Keyword(s):  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells

scholarly journals Soluble factors and the emergence of chick primordial germ cells in vitro

2000 ◽  
Vol 79 (1) ◽  
pp. 80-85 ◽  
Author(s):  
L. Karagenç ◽  
J.N. Petitte
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Soluble Factors

Chicken Model for Inducing Primordial Germ Cells Differentiating into Motile Sperms in vitro

2017 ◽  
Vol 10 (1) ◽  
pp. 12-18
Author(s):  
Hui Xiong ◽  
Xiangchen Li ◽  
Qingyun Hu ◽  
Pengfei Hu ◽  
Weijun Guan
Keyword(s):  
Flow Cytometry ◽  
Animal Model ◽  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Motile Sperm ◽  
Rt Pcr ◽  
Mechanism Research ◽  
Chicken Model

Stem cell technologies have been widely used in the study of spermatogenesis. However, deriving motile sperm from stem cells in vitro is still rarely achieved. We found that chicken primordial germ cells could directly differentiate into sperm by using retinoic acid in a non-testicular culture system. The induced sperms were characterised by RT-PCR, immunofluorescence and flow cytometry techniques. Results suggested that chicken primordial germ cells could produce motile sperm in vitro. Our work has provided a novel animal model of spermatogenesis in vitro, which might be used for male reproductive mechanism research.

scholarly journals Embryonic Fibroblast-Conditioned Medium Enhances Viability and Proliferation of Chick Circulating Primordial Germ Cells (cPGCs) in Suspension Culture

2002 ◽  
Vol 48 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Takaharu KAWASHIMA ◽  
Koichiro KANO ◽  
Yasuyuki KANNAN ◽  
Yoshinori TAMAKI ◽  
Koichiro HASHIMOTO
Keyword(s):  
Suspension Culture ◽  
Conditioned Medium ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Fibroblast

scholarly journals Culture of Chicken Gonadal Primordial Germ Cells (gPGCs) in Chicken Embryonic Fibroblast (CEF) Cells Conditioned Medium and in vivo Migration

2012 ◽  
Vol 11 (13) ◽  
pp. 2196-2203 ◽  
Author(s):  
Ming-Cheng Chang ◽  
Jenn-Fa Liou ◽  
Jen-Wen Shiau ◽  
Jui-Jane Tailiu ◽  
Chein Tai ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryonic Fibroblast ◽  
Embryonic Fibroblast
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