Skull growth in achondroplasic (cn) mice; a craniometric study

Development ◽  
1975 ◽  
Vol 33 (4) ◽  
pp. 1013-1022
Author(s):  
Rosemary J. Jolly ◽  
W. J. Moore
Keyword(s):  
Cranial Base ◽  
Septal Cartilage ◽  
Skull Morphology ◽  
Condylar Cartilage ◽  
Central Section ◽  
Condylar Process ◽  
Skull Growth ◽  
Anterior Extension ◽  
Posterior Extension ◽  
Nasal Septal Cartilage

Skull morphology in achondroplasic (cn/cn) mice was compared with that of normal siblings in order to determine the effects of this chondrodystrophy on skull growth, particular attention being given to dimensions reflecting growth at the synchondroses of the cranial base, the nasal septal cartilage and the condylar cartilage of the mandible. The central section of the cranial base (basicranial axis) was reduced by 25 %, the length of the viscerocranium by 18 % and the length of the condylar process by 11 %. The evidence indicates that these reductions are due to diminished growth at respectively the spheno-occipital and midsphenoidal synchondroses, the nasal septal cartilage and the condylar cartilage. The relative sizes of the reductions in cranial base, viscerocranium and condylar process suggest that the growth of synchondrotic and septal cartilages is diminished to a greater extent than that of condylar cartilage. This finding is in agreement with the observations that condylar cartilage, unlike synchondrotic and septal cartilage, grows by surface apposition and that the principal defect in cn/cn mice is a disturbance of interstitial cartilaginous growth. The posterior extension of the basicranial axis of the cn/cn mice was reduced by 14 % and the anterior extension by 2 %. The width of the cranial base was decreased by 9 % and the angle between the basicranial axis and its anterior extension was decreased by 3 %. The length of theneurocranium was reduced by 19 % in the cn/cn animals while the volume of the endocranial cavity was diminished by only 18 %. The latter reduction is less than would be expected from the cube relationship between volume and linear dimensions but is readily accounted for by the lack of reduction in the height or width of the neurocranium, the slight flattening of the cranial base and the doming of the neurocranial vault.

Further studies on skull growth in achondroplasic (cn) mice

Development ◽  
1977 ◽  
Vol 39 (1) ◽  
pp. 59-70
Author(s):  
A. K. Brewer ◽  
D. R. Johnson ◽  
W. J. Moore
Keyword(s):  
Direct Effect ◽  
Cranial Base ◽  
Long Bones ◽  
Percentage Reduction ◽  
Condylar Cartilage ◽  
Skull Growth ◽  
Basioccipital Bone

The morphology of the basioccipital, basisphenoid and mandibular bones in achondroplasic (cn/cn) mice was compared with that of normal siblings. The two bones of the cranial base were markedly reduced in length but not in width. The percentage reduction in the basisphenoid was twice that in the basioccipital bone and of the same magnitude as that previously observed in the long bones of the limbs. This difference may arise because the basisphenoid, like the long bones, grows in length from two cartilaginous growth sites while the basioccipital grows from one cartilaginous and one periosteal site. The mandible of the (cn/cn) mice was also reduced in size, although to a lesser extent than were the cranial bones and without the ensuing disproportion. The scale of the mandibular changes suggests that they are largely attributable to regulatory responses to the shortened cranium. The finding that the condylar cartilage of the cn/cn mice is reduced in thickness indicates, however, that the cn gene may have a direct effect on condylar chondrocytes.

The Effect of Parathyroid Hormone (1-34) on Cyclic AMP Level, Ornithine Decarboxylase Activity, and Glycosaminoglycan Synthesis of Chondrocytes from Mandibular Condylar Cartilage, Nasal Septal Cartilage, and Spheno-occipital Synchondrosis in Culture

1987 ◽  
Vol 66 (1) ◽  
pp. 84-87 ◽  
Author(s):  
T. Takano ◽  
M. Takigawa ◽  
E. Shirai ◽  
K. Nakagawa ◽  
M. Sakuda ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Ornithine Decarboxylase ◽  
Septal Cartilage ◽  
Decarboxylase Activity ◽  
Condylar Cartilage ◽  
Polyamine Biosynthesis ◽  
Glycosaminoglycan Synthesis ◽  
Mandibular Condylar Cartilage ◽  
Nasal Septal Cartilage

Previously, we reported methods for isolating chondrocytes from the craniofacial complex and their culture in vitro. The response of these chondrocyte cultures to bovine parathyroid hormone (1—34) (PTH) has now been investigated. PTH stimulated glycosaminoglycan (GAG) synthesis, a characteristic of the cartilage phenotype in cultured chondrocytes isolated from mandibular condylar cartilage (MCC), nasal septal cartilage (NSC), and spheno-occipital synchondrosis (SOS). These stimulations of GAG synthesis by PTH were dose-dependent. PTH also increased accumulation of cyclic AMP (cAMP) and the activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis. However, PTH did not stimulate DNA synthesis. The increases in the cAMP level, ODC activity, and GAG synthesis after addition of PTH (10-7 mol/L) were greatest in MCCchondrocytes and least in NSC-chondrocytes. The difference in the responses to PTH of these three types of chondrocytes may reflect differences of the characteristics of these cells in vivo.

scholarly journals Polydispersity in Sulfation Profile of Oligosaccharide Alditols Isolated from the Protein-Linkage Region and the Repeating Disaccharide Region of Chondroitin 4-Sulfate of Bovine Nasal Septal Cartilage

1996 ◽  
Vol 240 (3) ◽  
pp. 789-797 ◽  
Author(s):  
Tonny Beer ◽  
Atsuko Inui ◽  
Hiromi Tsuda ◽  
Kazuyuki Sugahara ◽  
Johannes F. G. Vliegenthart
Keyword(s):  
Septal Cartilage ◽  
Linkage Region ◽  
Nasal Septal Cartilage

Compensatory growth following premature closure of the coronal suture in rabbits

1982 ◽  
Vol 57 (4) ◽  
pp. 535-542 ◽  
Author(s):  
William J. Babler ◽  
John A. Persing ◽  
H. Richard Winn ◽  
John A. Jane ◽  
George T. Rodeheaver
Keyword(s):  
Compensatory Growth ◽  
Cranial Sutures ◽  
Coronal Suture ◽  
Skull Morphology ◽  
Content Type ◽  
Premature Closure ◽  
New Zealand White Rabbits ◽  
Skull Growth ◽  
Skull Deformation ◽  
Surgical Release

✓ Premature closure of a cranial suture results in skull deformation characterized by inhibited skull growth in a direction perpendicular to the course of the affected suture. Early surgical release of the closed suture results in a “normal” skull morphology. The present experimental study measured alterations in growth at the transverse cranial sutures that occurred following induced premature closure of the coronal suture and its subsequent release in New Zealand White rabbits. Using roentgenocephalometric methods, growth and morphometric changes were monitored at the frontonasal, coronal, and anterior lambdoid sutures following premature closure of the coronal suture at 9 days of age. The results indicated that premature closure of the coronal suture did not result in compensatory (increased) growth at the other transverse sutures of the vault. In fact, growth at these sutures was significantly reduced. This reduced growth at adjacent transverse sutures was not ameliorated by early surgical release of the fused suture.

scholarly journals Wound healing of autologous implants in the nasal septal cartilage —a verwoerd study

1997 ◽  
Vol 49 (1) ◽  
pp. 80-81
Author(s):  
D. S. Grewal ◽  
Dinaz K. Irani
Keyword(s):  
Wound Healing ◽  
Septal Cartilage ◽  
Nasal Septal Cartilage

scholarly journals Cellular proliferation in the nasal septal cartilage of juvenile minipigs

2014 ◽  
Vol 225 (6) ◽  
pp. 604-613 ◽  
Author(s):  
Ayman A. Al Dayeh ◽  
Susan W. Herring
Keyword(s):  
Cellular Proliferation ◽  
Septal Cartilage ◽  
Nasal Septal Cartilage

Tissue Engineered Human Septal Cartilage in a Murine Model

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P87-P88
Author(s):  
Angela Chang ◽  
Sage August ◽  
Barbara L Schumacher ◽  
Williams Gregory ◽  
Robert L Sah ◽  
...  
Keyword(s):  
Cell Viability ◽  
Murine Model ◽  
Three Dimensional ◽  
Alginate Beads ◽  
Septal Cartilage ◽  
Hematoxylin And Eosin ◽  
Matrix Production ◽  
Nasal Septal Cartilage

Problem Tissue engineering of human nasal septal cartilage represents an alternative technique for creating large quantities of autologous material for use in reconstructive surgery of the head and neck. Septal neocartilage constructs developed in vitro by the alginate method have demonstrated cartilaginous extracellular matrix production, but their biocompatibility and development in vivo remains largely unknown. Methods A murine model was used to examine the behavior of neocartilage constructs in vivo. Chondrocytes collected from donors undergoing septoplasty were expanded in monolayer and suspended in alginate beads for three-dimensional culture in media containing human serum and growth factors. After in vitro incubation for 5 weeks, the neocartilage constructs were implanted subcutaneously in the dorsum of athymic mice for 30 days (n=3). The mice were sacrificed and the constructs were explanted for assessment of cell viability, gross morphology, and histology. Results The mice survived and tolerated the implant well. Infection and extrusion were not observed. Neocartilage constructs maintained their general shape and size, and demonstrated cell viability after implantation. Explanted constructs were firm and opaque, sharing closer semblance to native septal tissue relative to the gelatinous, translucent pre-implant constructs. On hematoxylin and eosin staining, the explanted constructs exhibited distinct morphologies characteristic of native tissue, which were not observed in pre-implant constructs. Conclusion Neocartilage constructs are viable in an in vivo murine model. The morphologic and histologic features of explanted constructs more closely resemble native septal tissue when compared to pre-implant constructs. Significance Septal neocartilage constructs are biocompatible and demonstrate potential for in vivo maturation with eventual clinical application.

The width of nasal septal cartilage

2003 ◽  
Vol 129 (2) ◽  
pp. P256-P257
Author(s):  
A Ross
Keyword(s):  
Septal Cartilage ◽  
Nasal Septal Cartilage
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