scholarly journals Protein synthesis in the early Drosophila embryo; analysis of the protein species synthesized

Development ◽  
1977 ◽  
Vol 41 (1) ◽  
pp. 101-110
Author(s):  
David B. Roberts ◽  
Giorgio Graziosi
Keyword(s):  
Protein Synthesis ◽  
Total Protein ◽  
Soluble Proteins ◽  
Drosophila Embryo ◽  
Polyacrylamide Gels ◽  
Protein Species ◽  
Drosophila Development ◽  
Maternal Mrna ◽  
Egg Deposition ◽  
Early Drosophila Embryo

The soluble proteins were extracted from Drosophila eggs which had been permeabilized and incubated in medium containing [35S]methionine. These proteins were analysed on immunoelectrophoresis plates and on SDS polyacrylamide gels both by staining for total protein and by autoradiography. The radioactive proteins must have been synthesized during the period of incubation with [35S]methionine. In the period covered by this study (0–3 h) there was much protein synthesis but no new proteins were synthesized which had not already been synthesized during oogenesis. We conclude that the considerable protein synthesis that occurs in early Drosophila development is translated from maternal mRNA which is activated both by egg deposition and fertilization. Translation of protein from either masked maternal mRNA, which had not been previously translated, or from mRNA transcribed from the zygote genome must occur after blastoderm formation.

Quantitative Analysis of Gene Function in the Drosophila Embryo

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 273-284
Author(s):  
William D Tracey ◽  
Xiangqun Ning ◽  
Martin Klingler ◽  
Sunita G Kramer ◽  
J Peter Gergen
Keyword(s):  
Gene Function ◽  
Model Systems ◽  
Drosophila Embryo ◽  
Direct Role ◽  
Developmental Context ◽  
Maternal Mrna ◽  
Early Drosophila Embryo ◽  
Segment Polarity ◽  
Pair Rule

Abstract The specific functions of gene products frequently depend on the developmental context in which they are expressed. Thus, studies on gene function will benefit from systems that allow for manipulation of gene expression within model systems where the developmental context is well defined. Here we describe a system that allows for genetically controlled overexpression of any gene of interest under normal physiological conditions in the early Drosophila embryo. This regulated expression is achieved through the use of Drosophila lines that express a maternal mRNA for the yeast transcription factor GAL4. Embryos derived from females that express GAL4 maternally activate GAL4-dependent UAS transgenes at uniform levels throughout the embryo during the blastoderm stage of embryogenesis. The expression levels can be quantitatively manipulated through the use of lines that have different levels of maternal GAL4 activity. Specific phenotypes are produced by expression of a number of different developmental regulators with this system, including genes that normally do not function during Drosophila embryogenesis. Analysis of the response to overexpression of runt provides evidence that this pair-rule segmentation gene has a direct role in repressing transcription of the segment-polarity gene engrailed. The maternal GAL4 system will have applications both for the measurement of gene activity in reverse genetic experiments as well as for the identification of genetic factors that have quantitative effects on gene function in vivo.

scholarly journals Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo

Nature ◽  
2010 ◽  
Vol 467 (7319) ◽  
pp. 1128-1132 ◽  
Author(s):  
Christel Rouget ◽  
Catherine Papin ◽  
Anthony Boureux ◽  
Anne-Cécile Meunier ◽  
Bénédicte Franco ◽  
...  
Keyword(s):  
Drosophila Embryo ◽  
Maternal Mrna ◽  
Early Drosophila Embryo ◽  
Pirna Pathway

The Cytoskeleton of the Early Drosophila Embryo

1986 ◽  
Vol 1986 (Supplement 5) ◽  
pp. 311-328 ◽  
Author(s):  
R. M. WARN
Keyword(s):  
Drosophila Embryo ◽  
Early Drosophila Embryo
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