Protein synthesis patterns following stage-specific heat shock in early Drosophila embryo

1987 ◽  
Vol 210 (3) ◽  
pp. 407-412 ◽  
Author(s):  
Susan Eberlein ◽  
Herschel K. Mitchell
Keyword(s):  
Protein Synthesis ◽  
Heat Shock ◽  
Specific Heat ◽  
Drosophila Embryo ◽  
Early Drosophila Embryo

scholarly journals Protein synthesis in the early Drosophila embryo; analysis of the protein species synthesized

Development ◽  
1977 ◽  
Vol 41 (1) ◽  
pp. 101-110
Author(s):  
David B. Roberts ◽  
Giorgio Graziosi
Keyword(s):  
Protein Synthesis ◽  
Total Protein ◽  
Soluble Proteins ◽  
Drosophila Embryo ◽  
Polyacrylamide Gels ◽  
Protein Species ◽  
Drosophila Development ◽  
Maternal Mrna ◽  
Egg Deposition ◽  
Early Drosophila Embryo

The soluble proteins were extracted from Drosophila eggs which had been permeabilized and incubated in medium containing [35S]methionine. These proteins were analysed on immunoelectrophoresis plates and on SDS polyacrylamide gels both by staining for total protein and by autoradiography. The radioactive proteins must have been synthesized during the period of incubation with [35S]methionine. In the period covered by this study (0–3 h) there was much protein synthesis but no new proteins were synthesized which had not already been synthesized during oogenesis. We conclude that the considerable protein synthesis that occurs in early Drosophila development is translated from maternal mRNA which is activated both by egg deposition and fertilization. Translation of protein from either masked maternal mRNA, which had not been previously translated, or from mRNA transcribed from the zygote genome must occur after blastoderm formation.

The Cytoskeleton of the Early Drosophila Embryo

1986 ◽  
Vol 1986 (Supplement 5) ◽  
pp. 311-328 ◽  
Author(s):  
R. M. WARN
Keyword(s):  
Drosophila Embryo ◽  
Early Drosophila Embryo
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