Proliferation and agglutinability of primary and transformed human epithelial cells in culture

1976 ◽  
Vol 21 (3) ◽  
pp. 553-561
Author(s):  
M.A. Ricard ◽  
R.J. Hay
Keyword(s):  
Culture Medium ◽  
Monolayer Culture ◽  
Primary Cultures ◽  
Short Term ◽  
Chloromethyl Ketone ◽  
Normal Populations ◽  
Short Term Exposure ◽  
Cell Strains ◽  
Human Placentae

Primary epithelial populations (HAM) were obtained by dissociation of the amniotic membrane stripped from human placentae. Agglutinability of cells from such normal populations and of cells from the transformed epithelial line WISH was then compared using concavanalin A as mediator. Extensive similar studies have previously been reported with cell strains isolated from other species. Freshly dissociated HAM cells from primary cultures agglutinated much less readily than did cells from WISH populations. Furthermore, the former exhibited a drastic decline in agglutinability as a function of time in suspension culture after trypsinization. Short-term exposure (60 h) of HAM cells in monolayer culture to 5-bromodeoxyuridine (BrdU) elicited heightened agglutinability detectable through 22 days in vitro. Addition of the protease inhibitors n-tosyl-L-lysyl-chloromethyl ketone (TLCK) or p-tosyl-L-arginine-methyl ester (TAME) to the culture medium inhibited proliferation of the WISH line by 40–50% while effecting only a 10–15% inhibition of HAM cells. These results also confirm data with other cell species indicating that high proteolytic activity at the surface of transformed cells may be related to the rapid proliferation rate.

The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

1976 ◽  
Vol 36 (01) ◽  
pp. 221-229 ◽  
Author(s):  
Charles A. Schiffer ◽  
Caroline L. Whitaker ◽  
Morton Schmukler ◽  
Joseph Aisner ◽  
Steven L. Hilbert
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Dimethyl Sulfoxide ◽  
Platelet Function ◽  
Platelet Volume ◽  
Short Term ◽  
Platelet Factor ◽  
Short Term Exposure ◽  
Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

Pulsatile GnRH secretion from primary cultures of sheep olfactory placode explants

Reproduction ◽  
2000 ◽  
pp. 391-396 ◽  
Author(s):  
AH Duittoz ◽  
M Batailler
Keyword(s):  
Culture Medium ◽  
Primary Cultures ◽  
Pulsatile Secretion ◽  
Olfactory Placode ◽  
Gnrh Secretion ◽  
Individual Culture ◽  
Pulsatile Gnrh

The aim of this study was to investigate the development of pulsatile GnRH secretion by GnRH neurones in primary cultures of olfactory placodes from ovine embryos. Culture medium was collected every 10 min for 8 h to detect pulsatile secretion. In the first experiment, pulsatile secretion was studied in two different sets of cultures after 17 and 24 days in vitro. In the second experiment, a set of cultures was tested after 10, 17 and 24 days in vitro to investigate the development of pulsatile GnRH secretion in each individual culture. This study demonstrated that (i) primary cultures of GnRH neurones from olfactory explants secreted GnRH in a pulsatile manner and that the frequency and mean interpulse duration were similar to those reported in castrated ewes, and (ii) pulsatile secretion was not present at the beginning of the culture but was observed between 17 and 24 days in vitro, indicating the maturation of individual neurones and the development of their synchronization.

Soft agar cloning evaluation of effects of amifostine on clonogenic growth of freshly explanted human tumors in short term exposure in vitro

1997 ◽  
Vol 33 ◽  
pp. S178
Author(s):  
P. Pohl ◽  
H. Depenbrock ◽  
R. Peter ◽  
P. Schmid ◽  
J. Rastetter ◽  
...  
Keyword(s):  
Soft Agar ◽  
Human Tumors ◽  
Short Term ◽  
Clonogenic Growth ◽  
Short Term Exposure ◽  
Soft Agar Cloning

Über Antigenstrukturen von permanenten Zellstämmen

1960 ◽  
Vol 15 (11) ◽  
pp. 707-713 ◽  
Author(s):  
Arno Geissler ◽  
Else Knake
Keyword(s):  
Culture Medium ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Calf Serum ◽  
Antigenic Property ◽  
Fixation Test ◽  
Established Cell ◽  
Cell Strains ◽  
Original Species

Using the complement fixation test, we found three different antigenic components in established cell strains originating from man and mouse:1. the antigenic property of the original species specifity. It resides in cell substances which, in a serological sense, are haptenes.2. an acquired antigenic property of species specifity of beef, which is a haptene too. The cells acquired this antigenic component from substances in the culture medium containing 20% of calf serum.3. an antigenic property characterized as a complete antigen which, probably, was acquired by the cells only during their in vitro-life. The nature of this third antigen is not yet entirely clear.

scholarly journals Short-term storage in vitro and large-scale propagation of grapevine genotypes

2012 ◽  
Vol 47 (3) ◽  
pp. 344-350 ◽  
Author(s):  
Rafael de Carvalho Silva ◽  
Zanderluce Gomes Luis ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Survival Rate ◽  
Large Scale ◽  
Culture Medium ◽  
Short Term ◽  
Minimal Growth ◽  
Short Term Storage ◽  
Term Storage ◽  
Storage Temperatures ◽  
Number Of Shoots

The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L-1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20ºC, can be micropropagated in large scale.

Occurrence of molecular abnormalities of cell cycle in L132 cells after in vitro short-term exposure to air pollution PM2.5

2010 ◽  
Vol 188 (3) ◽  
pp. 558-565 ◽  
Author(s):  
Imane Abbas ◽  
Guillaume Garçon ◽  
Françoise Saint-Georges ◽  
Sylvain Billet ◽  
Anthony Verdin ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Air Pollution ◽  
Short Term ◽  
Molecular Abnormalities ◽  
Short Term Exposure

In vitro short-term exposure to air pollution PM2.5-0.3 induced cell cycle alterations and genetic instability in a human lung cell coculture model

2016 ◽  
Vol 147 ◽  
pp. 146-158 ◽  
Author(s):  
Imane Abbas ◽  
Anthony Verdin ◽  
Fabienne Escande ◽  
Françoise Saint-Georges ◽  
Fabrice Cazier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Air Pollution ◽  
Genetic Instability ◽  
Human Lung ◽  
Short Term ◽  
Short Term Exposure ◽  
Cell Cycle Alterations ◽  
Coculture Model

In vitro effects of silver nanoparticles on gills morphology of female Guppy ( Poecilia reticulate ) after a short‐term exposure

2020 ◽  
Vol 83 (12) ◽  
pp. 1552-1557 ◽  
Author(s):  
Reza Mohsenpour ◽  
Hamed Mousavi‐Sabet ◽  
Aliakbar Hedayati ◽  
Amir Rezaei ◽  
Ahmad Mohamadi Yalsuyi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Short Term ◽  
Short Term Exposure ◽  
In Vitro Effects
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