In vitro effects of silver nanoparticles on gills morphology of female Guppy ( Poecilia reticulate ) after a short‐term exposure

2020 ◽  
Vol 83 (12) ◽  
pp. 1552-1557 ◽  
Author(s):  
Reza Mohsenpour ◽  
Hamed Mousavi‐Sabet ◽  
Aliakbar Hedayati ◽  
Amir Rezaei ◽  
Ahmad Mohamadi Yalsuyi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Short Term ◽  
Short Term Exposure ◽  
In Vitro Effects

The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

1976 ◽  
Vol 36 (01) ◽  
pp. 221-229 ◽  
Author(s):  
Charles A. Schiffer ◽  
Caroline L. Whitaker ◽  
Morton Schmukler ◽  
Joseph Aisner ◽  
Steven L. Hilbert
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Dimethyl Sulfoxide ◽  
Platelet Function ◽  
Platelet Volume ◽  
Short Term ◽  
Platelet Factor ◽  
Short Term Exposure ◽  
Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

In vitro effects of methadone on lymphocyte proliferation following a short-term treatment with methotrexate-loaded liposomes in a murine model of arthritis

2006 ◽  
Vol 164 ◽  
pp. S110-S111
Author(s):  
Maria Barca ◽  
Anne Marie Ciobanu ◽  
Dan Balalau ◽  
Daniela Luiza Baconi ◽  
Mihaela Ilie ◽  
...  
Keyword(s):  
Murine Model ◽  
Lymphocyte Proliferation ◽  
Term Treatment ◽  
Short Term ◽  
Short Term Treatment ◽  
In Vitro Effects

Soft agar cloning evaluation of effects of amifostine on clonogenic growth of freshly explanted human tumors in short term exposure in vitro

1997 ◽  
Vol 33 ◽  
pp. S178
Author(s):  
P. Pohl ◽  
H. Depenbrock ◽  
R. Peter ◽  
P. Schmid ◽  
J. Rastetter ◽  
...  
Keyword(s):  
Soft Agar ◽  
Human Tumors ◽  
Short Term ◽  
Clonogenic Growth ◽  
Short Term Exposure ◽  
Soft Agar Cloning

Proliferation and agglutinability of primary and transformed human epithelial cells in culture

1976 ◽  
Vol 21 (3) ◽  
pp. 553-561
Author(s):  
M.A. Ricard ◽  
R.J. Hay
Keyword(s):  
Culture Medium ◽  
Monolayer Culture ◽  
Primary Cultures ◽  
Short Term ◽  
Chloromethyl Ketone ◽  
Normal Populations ◽  
Short Term Exposure ◽  
Cell Strains ◽  
Human Placentae

Primary epithelial populations (HAM) were obtained by dissociation of the amniotic membrane stripped from human placentae. Agglutinability of cells from such normal populations and of cells from the transformed epithelial line WISH was then compared using concavanalin A as mediator. Extensive similar studies have previously been reported with cell strains isolated from other species. Freshly dissociated HAM cells from primary cultures agglutinated much less readily than did cells from WISH populations. Furthermore, the former exhibited a drastic decline in agglutinability as a function of time in suspension culture after trypsinization. Short-term exposure (60 h) of HAM cells in monolayer culture to 5-bromodeoxyuridine (BrdU) elicited heightened agglutinability detectable through 22 days in vitro. Addition of the protease inhibitors n-tosyl-L-lysyl-chloromethyl ketone (TLCK) or p-tosyl-L-arginine-methyl ester (TAME) to the culture medium inhibited proliferation of the WISH line by 40–50% while effecting only a 10–15% inhibition of HAM cells. These results also confirm data with other cell species indicating that high proteolytic activity at the surface of transformed cells may be related to the rapid proliferation rate.

Short-term effect of aldosterone on NEM-sensitive ATPase in rat collecting tubule

1989 ◽  
Vol 257 (2) ◽  
pp. F177-F181 ◽  
Author(s):  
C. Khadouri ◽  
S. Marsy ◽  
C. Barlet-Bas ◽  
A. Doucet
Keyword(s):  
Atpase Activity ◽  
Affinity Constant ◽  
Short Term ◽  
Collecting Tubule ◽  
Lag Period ◽  
In Vitro Effects ◽  
Collecting Tubules

Because previous studies indicated that in the collecting tubule, N-ethylmaleimide (NEM)-sensitive ATPase, the biochemical equivalent of the proton pump, is controlled by mineralocorticoids in the long term, the present study was designed to investigate whether such control also exists in the short term. Therefore we investigated the in vivo and in vitro effects of aldosterone on the enzyme activity in cortical and outer medullary collecting tubules (CCT and MCT, respectively) from adrenalectomized rats. Administration of aldosterone (10 micrograms/kg body wt) markedly stimulated NEM-sensitive ATPase activity in the CCT and MCT within 3 h. Similarly, incubating CCT or MCT for 3 h in the presence of 10(-8) M aldosterone enhanced NEM-sensitive ATPase activity up to values similar to those previously measured in the corresponding nephron segments of normal rats. In vitro stimulation of NEM-sensitive ATPase was dose dependent in regard to aldosterone (apparent affinity constant approximately 10(-9) M), appeared after a 30-min lag period, and reached its maximum after 2-2.5 h. Finally, actinomycin D and cycloheximide totally abolished the in vitro action of aldosterone, demonstrating the involvement of protein synthesis in this process.

scholarly journals Murine leukemia inhibitory factor enhances retroviral-vector infection efficiency of hematopoietic progenitors

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1098-1103 ◽  
Author(s):  
FA Fletcher ◽  
DE Williams ◽  
C Maliszewski ◽  
D Anderson ◽  
M Rives ◽  
...  
Keyword(s):  
Leukemia Inhibitory Factor ◽  
Retroviral Vector ◽  
Inhibitory Factor ◽  
Hematopoietic Progenitors ◽  
Short Term ◽  
Infection Efficiency ◽  
Integration Sites ◽  
In Vitro Effects ◽  
Short Term Culture

Abstract We have investigated the in vitro effects of the cytokine leukemia inhibitory factor (LIF) on normal murine hematopoietic progenitors by measuring recovery and retroviral vector infection efficiency of 13-day posttransplant, spleen-colony-forming cell (CFU-S 13) in short-term culture. Up to a twofold increase in CFU-S13 recovery was observed, from 9.7 x 10(-5) cells in untreated controls to 17.8 to 19.5 x 10(-5) cells, depending on the concentration of LIF. Histologic analysis of spleen colonies from control and LIF-treated marrows demonstrated that there was no detectable alteration in the differentiative potential of CFU-S13. The efficiency of CFU-S13 infection was increased from 15% in untreated controls to 84% to 91% in LIF-treated marrows. Analysis of proviral integration sites in spleen colonies indicated that some CFU- S13 precursors were infected in the LIF-treated marrows.

Occurrence of molecular abnormalities of cell cycle in L132 cells after in vitro short-term exposure to air pollution PM2.5

2010 ◽  
Vol 188 (3) ◽  
pp. 558-565 ◽  
Author(s):  
Imane Abbas ◽  
Guillaume Garçon ◽  
Françoise Saint-Georges ◽  
Sylvain Billet ◽  
Anthony Verdin ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Air Pollution ◽  
Short Term ◽  
Molecular Abnormalities ◽  
Short Term Exposure

In vitro short-term exposure to air pollution PM2.5-0.3 induced cell cycle alterations and genetic instability in a human lung cell coculture model

2016 ◽  
Vol 147 ◽  
pp. 146-158 ◽  
Author(s):  
Imane Abbas ◽  
Anthony Verdin ◽  
Fabienne Escande ◽  
Françoise Saint-Georges ◽  
Fabrice Cazier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Air Pollution ◽  
Genetic Instability ◽  
Human Lung ◽  
Short Term ◽  
Short Term Exposure ◽  
Cell Cycle Alterations ◽  
Coculture Model

Short-term in vitro effects of bisphenol A activity on phenotype and function of peripheral blood immune system cells

2017 ◽  
Vol 110 ◽  
pp. 262-273 ◽  
Author(s):  
M. Zbucka-Kretowska ◽  
I. Poplawska ◽  
A. Kretowska ◽  
M. Moniuszko ◽  
K. Grubczak
Keyword(s):  
Immune System ◽  
Bisphenol A ◽  
Peripheral Blood ◽  
Short Term ◽  
In Vitro Effects ◽  
And Function
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