Long-term maintenance of reaggregated hypothalamic cultures developed from embryonic rat hypothalamus: prostaglandin release during synaptogenesis in vitro

1978 ◽  
Vol 34 (1) ◽  
pp. 159-171
Author(s):  
A. Gyevai ◽  
P.J. Chapple ◽  
W.H. Douglas
Keyword(s):  
Adult Animal ◽  
Morphological Differentiation ◽  
Adult Rat ◽  
Monolayer Cultures ◽  
Prostaglandin Release ◽  
Pge2 Concentration ◽  
The Media ◽  
Term Maintenance

Hypothalamic aggregate cultures were developed from hypothalami taken from rat embryos at 17–19 days gestation. The aggregate cultures exhibited a prominent morphological differentiation during 3–4 weeks in culture. The fine structure of the synapses formed in the aggregates resembled synapses in tha adult animal. During synaptogenesis the aggregates spontaneously release prostaglandin E2 (PGE2). The amount of PGE2 released in the media was reversed upon the morphological differentiation of the hypothalamic cultures. Media containing a higher PGE2 concentration increased the extracellular prolactin accumulation in monolayer cultures developed from adult rat hypophysis.

scholarly journals Gene transfer into hematopoietic stem cells: long-term maintenance of in vitro activated progenitors without marrow ablation.

1994 ◽  
Vol 91 (1) ◽  
pp. 350-354 ◽  
Author(s):  
D. Bienzle ◽  
A. C. Abrams-Ogg ◽  
S. A. Kruth ◽  
J. Ackland-Snow ◽  
R. F. Carter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
Marrow Ablation ◽  
Term Maintenance

Modification of an in vitro batch culture technique to improve precision in long-term studies

1998 ◽  
Vol 22 ◽  
pp. 323-325
Author(s):  
M. C. Hickey ◽  
A. P. Moloney ◽  
M. O'Connell ◽  
J. Connolly
Keyword(s):  
Batch Culture ◽  
Large Scale ◽  
Kinetic Studies ◽  
Culture Technique ◽  
The Media ◽  
Long Term Studies ◽  
Random Variation

In vitro techniques have been developed to facilitate the measurement of nutritional variability amongst food. Many kinetic studies have utilized the modified Tilley and Terry technique, with long-term incubations carried out in Erlenmeyer flasks. These are inefficient in utilizing incubator space for large scale studies. However substitution of Erlenmeyer flasks with tubes as fermentation units leaves the system prone to ‘bridging’, the formation of dense mats of forage particles by entrapped gas, above the level of the media in a fermentation unit. The objective of experiment 1 was to establish an effective incubation technique to eliminate the random variation caused by bridging.

A novel cultivation technique for long-term maintenance of bloodstream form trypanosomes in vitro

1995 ◽  
Vol 70 (1-2) ◽  
pp. 157-166 ◽  
Author(s):  
Friedemann Hesse ◽  
Paul M. Selzer ◽  
Kerstin Mühlstädt ◽  
Michael Duszenko
Keyword(s):  
Bloodstream Form ◽  
Cultivation Technique ◽  
Term Maintenance

Effect of thyroid hormones on angiotensinogen production in the rat in vivo and in vitro

1987 ◽  
Vol 115 (2) ◽  
pp. 311-315 ◽  
Author(s):  
M. Ruiz ◽  
M. Montiel ◽  
E. Jimenez ◽  
M. Morell
Keyword(s):  
Thyroid Hormones ◽  
Time Course ◽  
Plasma Renin ◽  
Rat Hepatocytes ◽  
In Vitro System ◽  
Adult Rat ◽  
Monolayer Cultures ◽  
Thyroid Dysfunctions

ABSTRACT The influence of thyroid hormones on angiotensinogen production was studied in vitro and in vivo. In the in-vitro system, angiotensinogen production rate (APR) of monolayer cultures of rat hepatocytes in response to tri-iodothyronine (T3) and thyroxine (T4) was assayed. In the in-vivo system, plasma angiotensinogen concentration (PAC) and liver angiotensinogen content (LAC) were measured in hyper- and hypothyroid rats. In both thyroid dysfunctions, a significant decrease of PAC was found compared with that in control animals; however, LAC showed a significant increase in hyperthyroidism and a marked decrease in hypothyroidism. As PAC is dependent upon both angiotensinogen production by the liver and angiotensinogen degradation by renin, the decrease in PAC observed in hyperthyroidism could be due to an increase in plasma renin concentration, which would overcome the increased synthesis of liver angiotensinogen observed in these animals. In fact, addition of various concentrations of T4 or T3 to monolayer cultures of adult rat hepatocytes significantly enhanced APR. This increase was greater and started earlier with T3 (1196·1 ± 143·7 (s.d.) pg/mg protein per 6-h incubation; significant differences at the third hour of incubation) than with T4 (858·3 ± 88·2 pg/mg protein per 6-h incubation; significant differences at the sixth hour of incubation). In addition, a close dose–response relationship was found in the cultures supplemented with T3. The different time-course in the response elicited by T3 and T4 on APR could be a consequence of the necessary transformation of T4 into T3 to acquire biological activity. J. Endocr. (1987) 115, 311–315

Pancreatic small cells: Analysis of quiescence, long-term maintenance and insulin expression in vitro

2007 ◽  
Vol 313 (5) ◽  
pp. 931-942 ◽  
Author(s):  
M. Petropavlovskaia ◽  
C.A. Bodnar ◽  
L.A. Behie ◽  
L. Rosenberg
Keyword(s):  
Small Cells ◽  
Insulin Expression ◽  
Term Maintenance

Evidence for functional sodium and calcium ion channels in the membrane of cultured cardiomyocytes of the adult rat

1983 ◽  
Vol 61 (11) ◽  
pp. 1312-1316 ◽  
Author(s):  
S. L. Jacobson ◽  
C. B. Kennedy ◽  
G. A. R. Mealing
Keyword(s):  
Action Potentials ◽  
Rat Cardiomyocytes ◽  
Adult Rat ◽  
Culture Cells ◽  
Calcium Ion Channels ◽  
Spontaneous Action ◽  
Fetal Bovine ◽  
Spontaneous Action Potentials

Characteristics are reported for electrical activity of adult rat cardiomyocytes in long-term primary culture. Cells in vitro for 12 to 28 days have mean membrane potential of −53 mV, are electrically excitable, and some are spontaneously contractile. The action potential of these cells has a slow rate of depolarization and is abolished by methoxyverapamil (D-600) but not by tetrodotoxin (TTX). When cells are hyperpolarized by passage of an inward current, spontaneous action potentials cease and action potentials evoked by depolarizing pulses are then TTX sensitive. Fetal bovine serum is a constituent of the culture medium. Its temporary removal causes spontaneous contractility to cease but the cells remain electrically excitable.

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