High-resolution scanning electron microscopy of human metaphase chromosomes

1982 ◽  
Vol 56 (1) ◽  
pp. 409-422 ◽  
Author(s):  
C.J. Harrison ◽  
T.D. Allen ◽  
M. Britch ◽  
R. Harris
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Three Dimensional ◽  
Loop Model ◽  
Giemsa Banding ◽  
Metaphase Chromosomes ◽  
Osmium Impregnation ◽  
Chromosome Integrity ◽  
Scanning Electron

Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy. Use of an osmium impregnation technique eliminated the need for sputter-coating and allowed high-resolution visualization of uncoated specimens. Chromosomes were of three-dimensional cylindrical profile, with well-defined chromatids and centromeres. Prior to Giemsa-banding a smooth surface morphology was observed. Relaxation of chromosome integrity by Giemsa-banding pretreatment allowed resolution of several orders of chromosome structure not previously demonstrated by scanning electron microscopy. The observed organization of the chromatin fibres allowed parallels to be drawn with the radial loop model of chromosome construction as described by Marsden and Laemmli.

scholarly journals High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.

1979 ◽  
Vol 83 (1) ◽  
pp. 249-254 ◽  
Author(s):  
W Ip ◽  
D A Fischman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Intermediate Filaments ◽  
Actin Filaments ◽  
Critical Point Drying ◽  
Osmium Impregnation ◽  
Cytoskeletal Elements ◽  
Scanning Electron

Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.

Morphometric, quantitative, and three-dimensional analysis of the heart muscle fibers of old rats: Transmission electron microscopy and high-resolution scanning electron microscopy methods

2012 ◽  
Vol 76 (2) ◽  
pp. 184-195 ◽  
Author(s):  
Diego Pulzatto Cury ◽  
Fernando José Dias ◽  
Marcia Consentino Kronka Sosthenes ◽  
Carlos Alexandre Dos Santos Haemmerle ◽  
Koichi Ogawa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Dimensional Analysis ◽  
Heart Muscle ◽  
Three Dimensional ◽  
Transmission Electron ◽  
Old Rats ◽  
Scanning Electron

scholarly journals High-resolution three-dimensional reconstruction of a whole yeast cell using focused-ion beam scanning electron microscopy

BioTechniques ◽  
2012 ◽  
Vol 53 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Dongguang Wei ◽  
Scott Jacobs ◽  
Shannon Modla ◽  
Shuang Zhang ◽  
Carissa L. Young ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Yeast Cell ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
Scanning Electron

High resolution detection of uncoated metaphase chromosomes by means of field emission scanning electron microscopy

Chromosoma ◽  
1994 ◽  
Vol 103 (6) ◽  
pp. 393-400 ◽  
Author(s):  
R. Rizzoli ◽  
E. Rizzi ◽  
M. Falconi ◽  
A. Galanzi ◽  
B. Baratta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Field Emission ◽  
Metaphase Chromosomes ◽  
Scanning Electron

High resolution detection of uncoated metaphase chromosomes by means of field emission scanning electron microscopy

Chromosoma ◽  
1994 ◽  
Vol 103 (6) ◽  
pp. 393-400 ◽  
Author(s):  
R. Rizzoli ◽  
E. Rizzi ◽  
M. Falconi ◽  
A. Galanzi ◽  
B. Baratta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Field Emission ◽  
Metaphase Chromosomes ◽  
Scanning Electron

Fine Structure of Visible Light Emitting Porous Silicon

1992 ◽  
Vol 283 ◽  
Author(s):  
Kiyokazu Nakagawa ◽  
Akio Nishida ◽  
Toshikazu Shimada
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Optical Microscopy ◽  
Three Dimensional ◽  
Photoluminescence Spectroscopy ◽  
Porous Si ◽  
Light Emitting ◽  
Si Films ◽  
Scanning Electron

ABSTRACTPorous Si formed by anodization is studied by means of high-resolution scanning electron microscopy, optical microscopy, micro-photoluminescence spectroscopy, high-resolution transmisson electron microscopy, and micro-Raman spectroscopy. Surface morphologies of porous Si films are varied by changing the anodization conditions. High-resolution scanning electron microscopy measurements show that in all these films, porous Si fine structures of visible photoluminescent regions, which are confirmed with optical microscopy and micro-photoluminescence spectroscopy, are composed of particlelike structure of various sizes from several nm to several tens of nm. Transmission electron microscopy shows that these particles are composed of single-crystal Si spheres covered with an oxide layer. The peak of the Raman spectrum from the particlelike structure is shifted to lower energy and is wider than that for bulk Si, possibly due to lattice expansion around large surface fractions. These results suggest that the effective photoluminescence of porous Si is due to three-dimensional carrier confinement in quantum boxes.

High resolution three dimensional imaging of placental villi using serial block face scanning electron microscopy

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 84
Author(s):  
Eleni Palaiologou ◽  
Wendy Chiu ◽  
Rodolfo Ribeiro de Souza ◽  
Patricia Goggin ◽  
Emma Lofthouse ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Face Scanning ◽  
Placental Villi ◽  
Block Face ◽  
Scanning Electron
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