scholarly journals A One-Step Enzyme-Linked Immunosorbent Assay for a Novel Osteoblast Differentiation-Promoting Compound, TAK-778 in Serum.

1999 ◽  
Vol 22 (12) ◽  
pp. 1266-1270 ◽  
Author(s):  
Tomofumi KUROKAWA ◽  
Tetsuo HOSHINO ◽  
Tsuneo ODA ◽  
Kazuhiro SAITO ◽  
Yasuaki OGAWA
Keyword(s):  
Immunosorbent Assay ◽  
Osteoblast Differentiation ◽  
Enzyme Linked Immunosorbent Assay ◽  
One Step

One Step Enzyme Linked Immunosorbent Assay for Direct Estimation of Serum Testosterone

2003 ◽  
Vol 24 (2) ◽  
pp. 205-217 ◽  
Author(s):  
Tulsidas G. Shrivastav ◽  
Anupam Basu ◽  
Kiran P. Kariya
Keyword(s):  
Immunosorbent Assay ◽  
Serum Testosterone ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Estimation ◽  
One Step

scholarly journals Wax-Assisted One-Step Enzyme-Linked Immunosorbent Assay on Lateral Flow Test Devices

2018 ◽  
Vol 34 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Masanori ISHII ◽  
Pattarachaya PREECHAKASEDKIT ◽  
Kentaro YAMADA ◽  
Orawon CHAILAPAKUL ◽  
Koji SUZUKI ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Lateral Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Flow Test ◽  
One Step ◽  
Lateral Flow Test

scholarly journals Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 551 ◽  
Author(s):  
Sato ◽  
Takaishi ◽  
Yasumoto ◽  
Watabe
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Specific Antibody ◽  
Metabolic Pathways ◽  
Keyhole Limpet Hemocyanin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa System ◽  
One Step ◽  
Analytical Procedures ◽  
Specific Polyclonal Antibody

A novel polyclonal antibody against tetrodotoxin (TTX) was raised using its haptenic antigen, where 4,9-anhydroTTX was reacted with 1,2-ethanedithiol and this derivative was further reacted with keyhole limpet hemocyanin (KLH). This newly designed antigen (KLH-TTX) was inoculated into rabbits, resulting in the production of the specific polyclonal antibody, which reacted well with TTX and its analogs, 4-epiTTX, 11-oxoTTX and 5,6,11-trideoxyTTX, except for 4,9-anhydroTTX. The enzyme-linked immunosorbent assay (ELISA) system using this specific antibody was also developed in the present study. This newly developed polyclonal antibody with analytical procedures using direct one-step ELISA is useful to detect TTX and its analogs in toxic organisms and also disclose the mechanisms involved in their metabolic pathways and accumulation of TTX.

A one-step enzyme-linked immunosorbent assay to detect anti-CMV antibodies; development and clinical validation

1987 ◽  
Vol 17 (1-2) ◽  
pp. 141-148 ◽  
Author(s):  
F. Wielaard ◽  
J. Scherders ◽  
C. Dagelinckx ◽  
P. Gausset
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Validation ◽  
One Step

One Step Enzyme Linked Immunosorbent Assay for Direct Estimation of Serum Cortisol

2000 ◽  
Vol 21 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Anupam Basu ◽  
Tulsidas G. Shrivastav
Keyword(s):  
Immunosorbent Assay ◽  
Serum Cortisol ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Estimation ◽  
One Step

scholarly journals Polymorphism of sarcoplasmic-reticulum adenosine triphosphatase of rabbit skeletal muscle

1981 ◽  
Vol 197 (1) ◽  
pp. 245-248 ◽  
Author(s):  
E Damiani ◽  
R Betto ◽  
S Salvatori ◽  
P Volpe ◽  
G Salviati ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Immunosorbent Assay ◽  
Adenosine Triphosphatase ◽  
Slow Muscle ◽  
Fast Muscle ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunological Relationship ◽  
One Step ◽  
Membrane Bound

Antibody was raised in chickens against purified sarcoplasmic-reticulum Ca2+-activated ATPase (Ca2+-ATPase). The immunological relationship between the Ca2+-ATPase of fast-muscle and slow-muscle sarcoplasmic reticulum was investigated by a one-step and a two-step competitive enzyme-linked immunosorbent assay (ELISA). The results show marked antigenic differences between the membrane-bound Ca2+-ATPase of the sarcoplasmic-reticulum vesicles from fast muscle and slow muscle, beside differences in the membrane content of ATPase protein.

One-step sandwich enzyme-linked immunosorbent assay of human tissue plasminogen activator using monoclonal antibodies

1985 ◽  
Vol 40 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Tsutomu Kaizu ◽  
Kayoko Kojima ◽  
Kaori Iwasaki ◽  
Tatsuo Yamashita
Keyword(s):  
Monoclonal Antibodies ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Human Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Plasminogen ◽  
One Step

scholarly journals Nanobody-Alkaline Phosphatase Fusion Protein-Based Enzyme-Linked Immunosorbent Assay for One-Step Detection of Ochratoxin A in Rice

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 4044 ◽  
Author(s):  
Zhichang Sun ◽  
Xuerou Wang ◽  
Qi Chen ◽  
Yonghuan Yun ◽  
Zongwen Tang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Limit Of Detection ◽  
Binding Capacity ◽  
Cross Reactivity ◽  
Solvent Tolerance ◽  
Enzyme Linked Immunosorbent Assay ◽  
One Step

Ochratoxin A (OTA) has become one a focus of public concern because of its multiple toxic effects and widespread contamination. To monitor OTA in rice, a sensitive, selective, and one-step enzyme-linked immunosorbent assay (ELISA) using a nanobody-alkaline phosphatase fusion protein (Nb28-AP) was developed. The Nb28-AP was produced by auto-induction expression and retained an intact antigen-binding capacity and enzymatic activity. It exhibited high thermal stability and organic solvent tolerance. Under the optimal conditions, the developed assay for OTA could be finished in 20 min with a half maximal inhibitory concentration of 0.57 ng mL−1 and a limit of detection of 0.059 ng mL−1, which was 1.1 times and 2.7 times lower than that of the unfused Nb28-based ELISA. The Nb28-AP exhibited a low cross-reactivity (CR) with ochratoxin B (0.92%) and ochratoxin C (6.2%), and an ignorable CR (<0.10%) with other mycotoxins. The developed Nb-AP-based one-step ELISA was validated and compared with a liquid chromatography-tandem mass spectrometry method. The results show the reliability of Nb-AP-based one-step ELISA for the detection of OTA in rice.

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