scholarly journals Cloning and Expression of Dcga Gene Encoding α Subunit of GTP-Binding Protein in Carrot Seedlings

2007 ◽  
Vol 30 (9) ◽  
pp. 1800-1804 ◽  
Author(s):  
Yuki Asakura ◽  
Fumiya Kurosaki
Keyword(s):  
Binding Protein ◽  
Cloning And Expression ◽  
Gtp Binding Protein ◽  
Gene Encoding ◽  
Α Subunit ◽  
Gtp Binding

Molecular cloning and expression of a small GTP-binding protein of the Rop family from mung bean

Plant Science ◽  
2006 ◽  
Vol 171 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Eun-Mi Eom ◽  
Jun-Kyong Cho ◽  
Seoung-Ok Lim ◽  
Youn-Jung Byun ◽  
Dong-Hee Lee
Keyword(s):  
Molecular Cloning ◽  
Mung Bean ◽  
Binding Protein ◽  
Cloning And Expression ◽  
Gtp Binding Protein ◽  
Gtp Binding

rha1, a Gene Encoding a Small GTP Binding Protein from Arabidopsis, Is Expressed Primarily in Developing Guard Cells

1993 ◽  
Vol 5 (12) ◽  
pp. 1761 ◽  
Author(s):  
Nancy Terryn ◽  
Marco Brito Arias ◽  
Gilbert Engler ◽  
Christine Tire ◽  
Raimundo Villarroel ◽  
...  
Keyword(s):  
Binding Protein ◽  
Guard Cells ◽  
Gtp Binding Protein ◽  
Gene Encoding ◽  
Gtp Binding

scholarly journals The Trimeric GTP-binding Protein (Gq/G11) α Subunit Is Required for Insulin-stimulated GLUT4 Translocation in 3T3L1 Adipocytes

2000 ◽  
Vol 275 (10) ◽  
pp. 7167-7175 ◽  
Author(s):  
Makoto Kanzaki ◽  
Robert T. Watson ◽  
Nikolai O. Artemyev ◽  
Jeffrey E. Pessin
Keyword(s):  
Binding Protein ◽  
Glut4 Translocation ◽  
Gtp Binding Protein ◽  
Α Subunit ◽  
Gtp Binding

scholarly journals Characterization of GTPase Activity of TrmE, a Member of a Novel GTPase Superfamily, from Thermotoga maritima

2000 ◽  
Vol 182 (24) ◽  
pp. 7078-7082 ◽  
Author(s):  
Kunitoshi Yamanaka ◽  
Jihwan Hwang ◽  
Masayori Inouye
Keyword(s):  
Thermotoga Maritima ◽  
Binding Protein ◽  
Protein A ◽  
Hydrolysis Rate ◽  
Gtpase Activity ◽  
Gtp Binding Protein ◽  
Gene Encoding ◽  
Gtp Hydrolysis ◽  
Gtp Binding

ABSTRACT A gene encoding a putative GTP-binding protein, a TrmE homologue that is highly conserved in both prokaryotes and eukaryotes, was cloned from Thermotoga maritima, a hyperthermophilic bacterium.T. maritima TrmE was overexpressed in Escherichia coli and purified. TrmE has a GTPase activity but no ATPase activity. The GTPase activity can be competed with GTP, GDP, and dGTP but not with GMP, ATP, CTP, or UTP. Km andk cat at 70°C were 833 μM and 9.3 min−1, respectively. Our results indicate that TrmE is a GTP-binding protein with a very high intrinsic GTP hydrolysis rate. We also propose that TrmE homologues constitute a novel subfamily of the GTPase superfamily.

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