scholarly journals A Standardized Method for the Preparation of a Gas Phase Extract of Cigarette Smoke

2016 ◽  
Vol 39 (6) ◽  
pp. 898-902 ◽  
Author(s):  
Tsunehito Higashi ◽  
Yosuke Mai ◽  
Yuichi Mazaki ◽  
Takahiro Horinouchi ◽  
Soichi Miwa
Science ◽  
1966 ◽  
Vol 153 (3741) ◽  
pp. 1248-1250 ◽  
Author(s):  
T. R. Walker ◽  
J. E. Kiefer
Keyword(s):  

Author(s):  
T. B. Williams

AbstractNitric oxide in cigarette smoke was conveniently determined by non-dispersive infrared analysis (NDIR). Recoveries of 95 % were obtained with standard gas-air mixtures but recoveries from smoke increased from 87% for high-yield to 91 % for low-yield cigarettes. Relative error was about 4 %. A reduction in the dead volume of Cambridge filter cassettes, to reduce the amount of NO reacted between puffs, increased NO deliveries of cigarettes by 4%. Deliveries of NO were estimated to average 4 % lower due to oxidation, but reaction with other smoke components reduced them further depending upon concentrations. The NO deliveries of cigarettes increased as blend nitrate increased and as the flow of air around cigarettes decreased. Nitric oxide in smoke and in standard gas-air mixtures, determined by non-dispersive infrared (NDIR) spectroscopy, was substantiated by an automated colorimetric analysis. Interfering smoke species were determined and circumvented in both methods.


Author(s):  
M. A. Perinelli ◽  
N. Carugno

Abstract A flameless atomic absorption method has been developed which permits the quick determination of the levels of trace metals in cigarette smoke. The total particulate matter (TPM) was collected by electrostatic precipitation and dissolved in methanol before analysis. In order to trap the last remaining traces of metals, the gas phase was passed through microporous filters which were analysed direct. The sensitivity of this method for the metals studied (Zn, Pb, Cd, Ni) is good in both the particulate and gas phase samples, with the sole exception of Ni in the gas phase. Results obtained by the flame and flameless methods are compared.


1992 ◽  
Vol 286 (2) ◽  
pp. 607-611 ◽  
Author(s):  
A Z Reznick ◽  
C E Cross ◽  
M L Hu ◽  
Y J Suzuki ◽  
S Khwaja ◽  
...  

Exposure of human plasma to gas-phase (but not to whole) cigarette smoke (CS) produces oxidative damage to lipids [Frei, Forte, Ames & Cross (1991) Biochem. J. 277, 133-138], which is prevented by ascorbic acid. The ability of CS to induce protein damage was measured by the carbonyl assay and by loss of enzyme activity and protein -SH groups. Both whole and gas-phase CS caused formation of carbonyls in human plasma, which was partially inhibited by GSH but not by ascorbic acid or metal-ion-chelating agents. Isolated albumin exposed to CS showed much faster carbonyl formation (per unit protein) than did whole plasma; damage to isolated albumin was partially prevented by chelating agents. Isolated creatine kinase (CK) lost activity upon exposure to CS much faster than did CK in plasma. Direct addition to plasma of mixtures of some or all of the aldehydes reported to be present in CS caused protein carbonyl formation and inactivation of CK, but neither occurred to the extent produced by CS exposure.


2008 ◽  
Vol 17 (Supplement 1) ◽  
pp. i10-i16 ◽  
Author(s):  
G M Polzin ◽  
L Zhang ◽  
B A Hearn ◽  
A D Tavakoli ◽  
C Vaughan ◽  
...  

Author(s):  
P.F. Collins ◽  
N.M. Sarji ◽  
J.F. Williams

AbstractA procedure for the combined determination of total HCN and total gas phase aldehydes in cigarette smoke has been developed which is practical to use for the analysis of relatively large cigarette samples. The smoking system includes a Cambridge pad for collection of the particulate phase and a small tube of silica gel to trap gas phase components, with 5 cigarettes being smoked through each pad and trap. Following smoking, the Cambridge pad and silica gel trap are extracted; the silica gel extract is used for the determination of total gas phase aldehydes and the combined extracts for HCN analysis. Colourimetric procedures, automated through use of the Technicon AutoAnalyser as described previously, are employed for the analysis of the sample extracts. Evaluation of this combined procedure indicates that it yields reliable results for both total HCN and total gas phase aldehydes with greater speed and ease of operation than provided by the previously described methods. The procedure has been applied to various cigarette samples with the relative standard deviation for a single port of 5 cigarettes ranging from 1.9 to 5.5 % for gas phase aldehydes and from 2.6 to 8.4 % for total HCN.


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