A fluorophotometric determination of serum creatinine and creatine using a creatinineamidohydrolase-creatineamidinohydrolase-sarcosine oxidase-peroxidase system and diacetyldichlorofluorescin.

Abstract Creatinine amidohydrolase is used to measure serum creatinine in a totally enzymatic procedure. Creatine, produced by hydrolysis, is acted upon by creatine kinase, and then by pyruvate kinase and lactate dehydrogenase, to result in a change in absorbance at 340 nm. The amount of creatinine present is related to the rate of change in A340 and is determined from a standard curve. Absorbance and concentration are linearly related to 100 mg/liter and only 250 µl of serum is required. At 1.0 g/liter, heparin, oxalate, citrate, ethylenediaminetetraacetate, ascorbate, or glucose had no significant effect on the accurate determination of creatinine; higher concentrations (30 g/liter) had inhibitory effects on the test. Analytical recovery of creatinine added to either normal or abnormal sera averaged 102%. When results of this procedure and of the standard direct Jaffé test were compared, the latter were significantly higher. Unlike the Jaffé method, the present method of determining creatinine is rapid (about 10 min per test), subject to few or no interfering substances, and requires no serum deproteinization.

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Fe2O3/polyaniline supramolecular nanocomposite: A receptor free sensor platform for the quantitative determination of serum creatinine

Analytica Chimica Acta ◽

10.1016/j.aca.2020.09.004 ◽

2020 ◽

Vol 1137 ◽

pp. 103-114

Author(s):

R. Sriramprabha ◽

M. Sekar ◽

R. Revathi ◽

C. Viswanathan ◽

J. Wilson

Keyword(s):

Serum Creatinine ◽

Quantitative Determination ◽

Sensor Platform

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Effect of detergent on kinetic Jaffé-method assay of creatinine.

Clinical Chemistry ◽

10.1093/clinchem/26.12.1729 ◽

1980 ◽

Vol 26 (12) ◽

pp. 1729-1732 ◽

Cited By ~ 20

Author(s):

A Bergman ◽

G Ohman

Keyword(s):

Mass Spectrometry ◽

Serum Creatinine ◽

Isotope Dilution ◽

Isotope Dilution Mass Spectrometry ◽

Reference Method

Abstract Using isotope dilution-mass spectrometry as reference method, we show that the kinetic Jaffé method for determination of serum creatinine sometimes produces results that are much too high. Such falsely increased values were obtained in about 10% of the sera collected for routine determination of creatinine, and were most common when certain types of collecting tubes were used. The interferences could be overcome by use of detergent in the reaction mixture.

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Comparison of methods for determination of glomerular filtration rate in hypertensive subjects with normal serum creatinine

Blood Pressure ◽

10.3109/08037051003718473 ◽

2010 ◽

Vol 19 (5) ◽

pp. 278-286 ◽

Cited By ~ 2

Author(s):

Alberto Mazza ◽

Domenico Montemurro ◽

Antonio Piccoli ◽

Antonio Pagnan ◽

Achille Cesare Pessina ◽

...

Keyword(s):

Glomerular Filtration Rate ◽

Serum Creatinine ◽

Glomerular Filtration ◽

Filtration Rate ◽

Normal Serum ◽

Comparison Of Methods ◽

Normal Serum Creatinine

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Challenge and limitations in determination of serum creatinine

Orvosi Hetilap ◽

10.1556/oh.2008.28262 ◽

2008 ◽

Vol 149 (7) ◽

pp. 317-323 ◽

Cited By ~ 1

Author(s):

Anna V. Oláh ◽

Bertalan Fodor ◽

Andrea Horváth

Keyword(s):

Renal Disease ◽

Serum Creatinine

A 2006. évi ajánlások szerint az alapellátásban is alkalmazni kell a szérumkreatininből képlettel számított glomerulus filtrációs rátát, amely segít a krónikus vesebetegség korai felismerésében. Emiatt előtérbe kerültek a Jaffe-féle kreatininmeghatározás korlátai, és az eredmények összevethetősége érdekében felmerült az igény a leggyakrabban használt Jaffe- és az újabb kreatininmeghatározások harmonizációjára. A kinetikus Jaffe-féle kreatininmeghatározás hátránya, hogy nem elég specifikus. Ezen javítottak az elmúlt évtizedben bevezetett enzimatikus, kompenzált Jaffe és nagyfelbontású folyadékkromatográfiás módszerek. Az újabb módszerekkel kapott kreatininértékek már pontosabbak, de alacsonyabb értékük miatt a korábbi formulákkal (4 változós Modification of Diet in Renal Disease-186, Cockcroft–Gault, kvadratikus) nem lehet belőlük glomerulus filtrációs rátát számítani, mert durván torzítanák azt. Az új kreatininmódszerek miatt a korábbi számítási mód, s így a krónikus vesebetegség stádiumának meghatározása is bizonytalanná vált. Emiatt 2006-ban módosították a 4-változós Modification of Diet in Renal Disease-186 formulát, és az új (izotópdilutiós tömegspektrometriás referenciamódszerre visszavezethető) kreatininhez csak az új 4 változós Modification of Diet in Renal Disease-175 képlet ajánlott. A szerzők összevetik a kreatininmódszerek diagnosztikai értékét és korlátait.

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Definitive liquid-chromatographic demonstration that N-ethylglycine is the metabolite of lidocaine that interferes in the Kodak sarcosine oxidase-coupled method for creatinine.

Clinical Chemistry ◽

10.1093/clinchem/34.12.2569 ◽

1988 ◽

Vol 34 (12) ◽

pp. 2569-2572 ◽

Cited By ~ 6

Author(s):

R T Roberts ◽

N M Alexander ◽

M J Kelner

Keyword(s):

Serum Creatinine ◽

Human Serum ◽

Sarcosine Oxidase ◽

Coupled Method ◽

Linear Relationships ◽

Liquid Chromatographic ◽

Drug Free ◽

Single Slide

Abstract Patients receiving lidocaine may show false increases of serum creatinine as assayed by the single-slide method on the Kodak Ektachem 700. Bissell et al. (Clin Chem 1987;33:951) suggested that this interference was due to oxidation of N-ethylglycine (NEG), a previously uncharacterized metabolite of lidocaine, by the sarcosine oxidase preparation used in the Ektachem creatinine slide. To investigate this possibility, we synthesized NEG, added it to drug-free human serum, and analyzed the NEG-supplemented sera for creatinine with the Ektachem 700. We found the following linear relationships between creatinine bias (y, mg/L) and NEG concentration (x, mg/L) for first (I), third (III), and fourth (IV) generation slides: I: y = 1.70x - 0.8 mg/L (n = 13, r = 1.0) III: y = 0.39x - 0.3 mg/L (n = 3, r = 1.0) IV: y = 0.79x - 1.8 mg/L (n = 13, r = 1.0) Using HPLC, we directly demonstrated the presence of NEG in sera of patients receiving lidocaine and quantified NEG concentrations in sera from four of these patients. The increasing artifactual bias in creatinine with increasing NEG concentration unequivocally confirmed that NEG is responsible for the lidocaine-associated interference in the Kodak Ektachem single-slide creatinine method.

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