Determination of serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase by using L-glutamate oxidase.

1986 ◽  
Vol 34 (1) ◽  
pp. 409-412 ◽  
Author(s):  
TOSHIO KAMEI ◽  
KAZUKO ASANO ◽  
SHOSHIRO NAKAMURA
Keyword(s):  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Glutamate Oxidase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

scholarly journals EVALUATION OF ANTI-HYPERTROPHIC POTENTIAL OF CAMELLIA SINENSIS IN ISOPROTERENOL INDUCED CARDIAC HYPERTROPHY

2018 ◽  
Vol 10 (10) ◽  
pp. 119
Author(s):  
Doss V. A. ◽  
Jeevitha Parthibhan ◽  
Dharaniyambigai Kuberapandian
Keyword(s):  
Lactate Dehydrogenase ◽  
Cardiac Hypertrophy ◽  
Aqueous Extract ◽  
Marker Enzymes ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Cardiac Marker ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Objective: Camellia sinensis (C. sinensis family-Theaceae) has potent antioxidant activity used in the treatment of cardiovascular disease. The present study evaluates the cardioprotective (anti-hypertrophic) effect of aqueous extract of C. sinensis in isoproterenol (ISO) induced cardiac hypertrophic rats.Methods: The beneficial effect of the green tea extract was examined by the administration of the aqueous extract of the leaves of C. sinensis (100 mg/kg b.w., oral., 7 d) in ISO (10 mg/kg b.w., subcutaneous.,7 d) induced cardiac hypertrophic rats with reference to the standard drug, losartan (50 mg/kg b.w., oral.,7 d) followed by biochemical estimations of glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and lactate dehydrogenase (LDH) in serum and heart tissues thus collected at the end of 7 d.Results: The biochemical assays revealed significantly (P<0.05) increased glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) lactate dehydrogenase (LDH) and significantly (P<0.05) decreased in ISO induced cardiac hypertrophic rats that were reciprocated by the effect of plant extract.Conclusion: Thus, this study showed that the aqueous leaf extract of C. sinensis possesses potent effect against cardiac hypertrophy. This potential is hypothesized to be due to the phytochemical, Catechin present in the plant that requires further isolation and characterization with respect to anti-hypertrophic therapeutics.

scholarly journals HEPATOPROTECTIVE EFFECTS OF PUNICA GRANATUM FRUIT AGAINST D-GALACTOSAMINE-INDUCED HEPATOTOXICITY IN RATS: IN VITRO AND IN VIVO STUDIES

2017 ◽  
Vol 9 (8) ◽  
pp. 168
Author(s):  
Mohd Amir ◽  
Mohd Nasar Mallick ◽  
Niyaz Ahmad ◽  
Abuzer Ali ◽  
Sayeed Ahmad ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Vitro Study ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Objective: Hepatoprotective activity of Punica granatum Linn. (Family: Punicaceae) was evaluated by in vitro and in vivo model. HepG2 cell lines were used for in vitro study and D-Galactosamine (D-GalN) induced hepatic damage model for in vivo evaluation.Methods: Hepatoprotective potential was assessed by measuring serum level of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, total bilirubin, and albumin. Enzymatic antioxidant parameters like TBARs (thiobarbituric acid reactive substances), GSH (glutathione), SOD (superoxide dismutase) and CAT (catalase) also evaluated in this study. Results: Rats treated with D-GalN showed a significant increase in serum levels of SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase), ALP (alkaline phosphatase), bilirubin and TBARs, reflecting liver damage. The in vitro study indicates a beneficial effect of aqueous extract of P. granatum in comparison with methanolic extract, on D-GalN induced toxicity to HepG2 cells. On the basis of in vitro study, aqueous extract was selected for in vivo evaluation. The aqueous extract of P. granatum significantly reduced the elevated serum biomarkers, indicating the recovery of hepatocellular injury. It was also observed that D-GalN induced a significant decrease in GSH, SOD, CAT, protein, and albumin level were increased on the treatment of the rats with aqueous extract of P. granatum. The findings were also confirmed by histopathological studies. Periportal area and extensive hepatocyte damage and haemorrhage are seen in D-GalN treated group. The portal triad with less degree of inflammatory cell infiltration around the bile duct is seen in P. granatum (500 mg/kg)+(D-GalN) treated group.Conclusion: Results of this study revealed that P. granatum fruit could afford a significant protection for the alleviation of hepatic toxicity. Possible mechanism may involve its action against oxidative stress. 

scholarly journals Effect of rhizomes of Curculigo orchioides on Thioacetamide induced hepatotoxicity

2014 ◽  
Vol 15 (1&2) ◽  
pp. 55-60
Author(s):  
Somesh Thapliyal ◽  
Vijay Juyal ◽  
Anil Bhandari
Keyword(s):  
Total Bilirubin ◽  
Serum Alkaline Phosphatase ◽  
Hepatoprotective Activity ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Once Daily ◽  
Curculigo Orchioides ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The hepatoprotective activity of methanolic rhizome extract of Curculigo orchioides (MECO) were evaluated against Thioacetamide-induced hepatic damage in rats. The MECO at dose of 100, 200 and 400 mg/kg were administered orally once daily for 21 days and simultaneously administered TAA 100 mg/kg b.w. s.c. 1 h after the respective assigned treatments every 72 h.  Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues.  

scholarly journals Effect of rosella (Hibiscus sabdariffa L) extract on glutathione-S-transferase activity in rats

2017 ◽  
Vol 16 (10) ◽  
pp. 2411-2416
Author(s):  
Laela Hayu Nurani Nurkhasanah ◽  
Zainur Rahman Hakim
Keyword(s):  
Kinetic Method ◽  
Control Group ◽  
Transferase Activity ◽  
Hibiscus Sabdariffa ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Group I ◽  
Glutamate Pyruvate ◽  
Serum Glutamate ◽  
Gst Activity

Purpose: To determine the effect of rosella (Hibiscus sabdariffa L) extract on  glutathione-S-trasferase (GST) activity and its hepatoprotective effect.Methods: A total of 25 rats were divided randomly into 5 groups (5 rats per group). Group I served as the baseline, group II was the negative control group, while groups III, IV and V were treated with rosella extract at doses of 10, 50 and 100 mg /kg /day, respectively for 35 days. On day 36, the animals were given a single dose of dimethyl benz(a)anthracene (DMBA) orally. After one week, blood was taken from the sinus orbitalis for measurement of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) activities by a kinetic method with DIasys® kit. The activity of GST was measured in liver  homogenate using 1-choloro-2,4-dinitrobenzene (CDNB) method, while the  expression of GST gene was determined by reverse transcriptase polymerase chain reaction (RT-PCR).Results: Treatment with rosella extract at 10, 50 and 100 mg /kg for 35 days led to significant increasesin GST activity relative to the control group. In addition, serum SGPT and SGOT activities were significantly decreased. There were significant increases in the expression of GST gene as evidenced  by increased GST band intensity.Conclusion: These results indicate that rosella possesses significant  hepatoprotective effect against hepatic injury caused by DMBA treatment. Thus, rosella may be useful for the prevention of oxidative stress caused by free radicals produced from pollutants and foods.Keywords: Hibiscus sabdariffa, Rosella, Glutamate pyruvate transaminase (SGPT),  Glutamate oxaloacetate transaminase (SGOT), glutathion-S-transferase (GST)

scholarly journals Hepatoprotective Effects of Thespesia lampas Dalz & Gibs in CCl4 Induced Liver Injury in Rats

1970 ◽  
Vol 7 (1) ◽  
pp. 11-13 ◽  
Author(s):  
B Sangameswaran ◽  
Chumbhale Deshraj ◽  
BR Balakrishnan ◽  
B Jayakar
Keyword(s):  
Carbon Tetrachloride ◽  
Tween 80 ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Chronic Liver Damage ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The extracts of the roots of Thespesia lampas (Malvaceae) were evaluated for hepatoprotective activity in rats by inducing chronic liver damage by subcutaneous injection of 50% v/v carbon tetrachloride in Tween 80 at a dose of 3ml/kg for a period of 4 weeks. The biochemical parameters like serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), serum bilirubin and total proteins were estimated to assess the liver function. Hepatic steatosis, centrilobular necrosis, and often swelling of the hepatic cytoplasm were observed in carbontetra chloride treated group, while these were completely absent in the extracts of T. lampas (300 mg/kg b.wt) treated groups (p < 0.01). The present investigation established pharmacological evidence to support the folkloric claim of hepatoprotective activity of T. lampas.Key words: Thespesia lampas, Carbon tetrachloride, Hepatoprotective, Rats, Root extracts  DOI = 10.3329/dujps.v7i1.1201Dhaka Univ. J. Pharm. Sci. 7(1): 11-13, 2008 (June)

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