Role of Curculigo Orchioides in the Proliferation and Apoptosis of Breast Cancer Michigan Cancer Foundation-7 (MCF-7) Cells Under Network Pharmacology

Based on network pharmacology (NP), the effects of curculigo orchioides (CO) granules alone or CO granules along with tamoxifen on the proliferation and apoptosis (P&A) of human breast cancer (BC) cells were explored. A search database was constructed to obtain the active components (ACs) of CO and related target genes to perform disease mapping, and a BC target map was constructed to perform related pathway enrichment analysis (PEA). The blank control group (CG), estrogen group (EG), and tamoxifen group (TG) were set as controls to observe the effects of CO granules alone and CO granules along with tamoxifen. The PEA showed that the effect of CO on BC may be related to the cooperation of its ACs. The target of action may be related to cell cycle and proliferation, growth factors, metabolic pathways, etc. Different concentrations of CO had different effects, CO granules of various concentrations had no obvious growth-promoting effect (P < 0.05), and so did CO combined with tamoxifen (P > 0.05); high-dose (H-D) CO combined with tamoxifen can induce cell apoptosis (P < 0.05). Through NP, the ACs of CO and related targets of BC were predicted and analyzed. At the same time, it was proved that CO granules are safe in clinical use, and the combined effect of tamoxifen is better in the treatment of estrogen-positive patients, providing reference for the follow-up use of CO in the treatment of BC.

Development of An Efficient Protocol for Rapid Propagation of Curculigo orchioides Using Leaf Explant Culture

Curculigo orchioides is one of the most common medicinal plants used by diverse cultures and tribal groups. The roots of the plant are used medicinally in Asian countries. Curculigo orchioides have the ability to regenerate through seeds and tubers, but the regeneration rate is low. Plant tissue culture method was believed to have potential for rapid multiplication of this medicinal plant. An efficient protocol for rapid propagation of Curculigo orchioides, of the family Amaryllidaceae, was developed using leaf explants culture. The leaf explants (1 cm x 1 cm squares) cultured on Murashige and Skoog (MS) basal medium were supplemented with various concentrations and combinations of auxins and cytokinins with temperature 25 ± 2°C, relative humidity 85-90% and photoperiod of 12 hours light (2000-3000 lux). Callus induction was obtained within 4 weeks, 2,4-D at 3 mg/l formed profuse callus and the degree was found to be the highest (+++) among all the treatments. The best response to shoot induction, with maximum shoot number 5.33 (mean number of shoots per explant) was obtained using 1.0 mg/l 6-benzyl aminopurine (BAP) in combination with 1.0 mg/l Kinetin. In vitro shoots were induced for rooting on 0.5 mg/l of NAA supplemented medium. In order for seedlings propagated in vitro to adapt to natural conditions, plants were growned on a substrate coir: husk ash: sand (with the ratio of 0.5: 0.5: 1) in a greenhouse (humidity: 70%, temperature: 28-300C) gave 88.33% survival rate after 8 weeks of culture. With the results received, this is an effective approach to propagating Curculigo orchioides.

Anti-Amnesic Effect of Curculigo orchioides Plant Extract and Its Function on Acetylcholinesterase Enzyme

Background: Alzheimer’s disease (AD) is a progressive neurodegenerative disorder, and one of the most predominant causes of dementia in older people. Preventing acetylcholine from breakdown by an excess amount of acetylcholinesterase is a proven therapy for AD. Traditionally Curculigo orchioides is known for its antioxidant activity, enzyme inhibition activity, and other medicinal uses. In this study, in vitro acetylcholinesterase, inhibitory activity was investigated, and methanolic extract of the plant showed significant activity. This study aims to investigate cholinesterase inhibitory activity, learning, and memory-enhancing activity of Curculigo orchioides. Materials and Methods: The crude methanol extract of the dried powder was prepared by the cold extraction method. In-vitro acetylcholinesterase (AChE) inhibitory activities were determined by the modified Ellman’s method. To confirm learning and memory-enhancing effects, the scopolamine-induced memory impairment Swiss-albino mice were used, and to find the anti-amnesic effect of the extract, a passive shock avoidance task was applied. Results: Results proved that scopolamine-induced cognitive dysfunction decreased significantly by administration of the plant extract solution in the passive avoidance task, and inhibited brain acetylcholinesterase activity. While running an in-vitro AChE inhibitory test the IC50 was 0.782±0.067μg/ml. Conclusion: The results suggested that methanolic extract of Curculigo orchioides can inhibit AChE in-vitro. Besides this, it provides data that proves its ability to improve whole brain AChE activity and enhance memory.

Optimization of culture conditions for callus proliferation of Curculigo orchioides Gaertn.

Curculigo orchioides Gaertn is a herbaceous plant that has long been used as a tonic in Vietnam with noticeable health benefits. However, the demand for rhizomes of this species could not be met due to their decreasing number in natural habitats. Despite its vulnerability, there are still not enough researches on producing calli of C. orchioides, which is a method having the capacity of creating a large source of cell biomass for bioactive compounds’ extractions. Thus, this study was conducted to figure out the best conditions for C. orchioides’s callus proliferation. Different light regimes, mineral media, and concentrations of some factors like kinetin (KIN), α-naphthaleneacetic acid (NAA), yeast extract (YE), activated charcoal (AC), and silver nitrate (AgNO3) were examined. It is shown by the results that half-strength MS medium (½ MS) given 0.5 mg/L KIN and MS medium supplemented with 0.5 mg/L KIN and put in the dark (0 light hour : 24 dark hours) are the optimal conditions for callus proliferation, with the highest fresh weights (FWs), dry weights (DWs), and growth indices (GIs) of 3.89 g / 0.45 g / 7.78, and 4.10 g / 0.47 g / 8.20, respectively. Additionally, the inhibitory effects of AgNO3, YE, and AC were demonstrated since there was no observed heavy callus in the media containing those factors.