scholarly journals EVALUATION OF ANTI-HYPERTROPHIC POTENTIAL OF CAMELLIA SINENSIS IN ISOPROTERENOL INDUCED CARDIAC HYPERTROPHY

2018 ◽  
Vol 10 (10) ◽  
pp. 119
Author(s):  
Doss V. A. ◽  
Jeevitha Parthibhan ◽  
Dharaniyambigai Kuberapandian
Keyword(s):  
Lactate Dehydrogenase ◽  
Cardiac Hypertrophy ◽  
Aqueous Extract ◽  
Marker Enzymes ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Cardiac Marker ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Objective: Camellia sinensis (C. sinensis family-Theaceae) has potent antioxidant activity used in the treatment of cardiovascular disease. The present study evaluates the cardioprotective (anti-hypertrophic) effect of aqueous extract of C. sinensis in isoproterenol (ISO) induced cardiac hypertrophic rats.Methods: The beneficial effect of the green tea extract was examined by the administration of the aqueous extract of the leaves of C. sinensis (100 mg/kg b.w., oral., 7 d) in ISO (10 mg/kg b.w., subcutaneous.,7 d) induced cardiac hypertrophic rats with reference to the standard drug, losartan (50 mg/kg b.w., oral.,7 d) followed by biochemical estimations of glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and lactate dehydrogenase (LDH) in serum and heart tissues thus collected at the end of 7 d.Results: The biochemical assays revealed significantly (P<0.05) increased glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) lactate dehydrogenase (LDH) and significantly (P<0.05) decreased in ISO induced cardiac hypertrophic rats that were reciprocated by the effect of plant extract.Conclusion: Thus, this study showed that the aqueous leaf extract of C. sinensis possesses potent effect against cardiac hypertrophy. This potential is hypothesized to be due to the phytochemical, Catechin present in the plant that requires further isolation and characterization with respect to anti-hypertrophic therapeutics.

scholarly journals HEPATOPROTECTIVE EFFECTS OF PUNICA GRANATUM FRUIT AGAINST D-GALACTOSAMINE-INDUCED HEPATOTOXICITY IN RATS: IN VITRO AND IN VIVO STUDIES

2017 ◽  
Vol 9 (8) ◽  
pp. 168
Author(s):  
Mohd Amir ◽  
Mohd Nasar Mallick ◽  
Niyaz Ahmad ◽  
Abuzer Ali ◽  
Sayeed Ahmad ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Vitro Study ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Objective: Hepatoprotective activity of Punica granatum Linn. (Family: Punicaceae) was evaluated by in vitro and in vivo model. HepG2 cell lines were used for in vitro study and D-Galactosamine (D-GalN) induced hepatic damage model for in vivo evaluation.Methods: Hepatoprotective potential was assessed by measuring serum level of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, total bilirubin, and albumin. Enzymatic antioxidant parameters like TBARs (thiobarbituric acid reactive substances), GSH (glutathione), SOD (superoxide dismutase) and CAT (catalase) also evaluated in this study. Results: Rats treated with D-GalN showed a significant increase in serum levels of SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase), ALP (alkaline phosphatase), bilirubin and TBARs, reflecting liver damage. The in vitro study indicates a beneficial effect of aqueous extract of P. granatum in comparison with methanolic extract, on D-GalN induced toxicity to HepG2 cells. On the basis of in vitro study, aqueous extract was selected for in vivo evaluation. The aqueous extract of P. granatum significantly reduced the elevated serum biomarkers, indicating the recovery of hepatocellular injury. It was also observed that D-GalN induced a significant decrease in GSH, SOD, CAT, protein, and albumin level were increased on the treatment of the rats with aqueous extract of P. granatum. The findings were also confirmed by histopathological studies. Periportal area and extensive hepatocyte damage and haemorrhage are seen in D-GalN treated group. The portal triad with less degree of inflammatory cell infiltration around the bile duct is seen in P. granatum (500 mg/kg)+(D-GalN) treated group.Conclusion: Results of this study revealed that P. granatum fruit could afford a significant protection for the alleviation of hepatic toxicity. Possible mechanism may involve its action against oxidative stress. 

scholarly journals Effect of rhizomes of Curculigo orchioides on Thioacetamide induced hepatotoxicity

2014 ◽  
Vol 15 (1&2) ◽  
pp. 55-60
Author(s):  
Somesh Thapliyal ◽  
Vijay Juyal ◽  
Anil Bhandari
Keyword(s):  
Total Bilirubin ◽  
Serum Alkaline Phosphatase ◽  
Hepatoprotective Activity ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Once Daily ◽  
Curculigo Orchioides ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The hepatoprotective activity of methanolic rhizome extract of Curculigo orchioides (MECO) were evaluated against Thioacetamide-induced hepatic damage in rats. The MECO at dose of 100, 200 and 400 mg/kg were administered orally once daily for 21 days and simultaneously administered TAA 100 mg/kg b.w. s.c. 1 h after the respective assigned treatments every 72 h.  Serum enzymatic levels of serum glutamate oxaloacetate transaminase (AST), serum glutamate pyruvate transaminase (ALT), serum alkaline phosphatase (ALP) and total bilirubin were estimated along with estimation of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissues.  

scholarly journals Hepatoprotective Effects of Thespesia lampas Dalz & Gibs in CCl4 Induced Liver Injury in Rats

1970 ◽  
Vol 7 (1) ◽  
pp. 11-13 ◽  
Author(s):  
B Sangameswaran ◽  
Chumbhale Deshraj ◽  
BR Balakrishnan ◽  
B Jayakar
Keyword(s):  
Carbon Tetrachloride ◽  
Tween 80 ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Chronic Liver Damage ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The extracts of the roots of Thespesia lampas (Malvaceae) were evaluated for hepatoprotective activity in rats by inducing chronic liver damage by subcutaneous injection of 50% v/v carbon tetrachloride in Tween 80 at a dose of 3ml/kg for a period of 4 weeks. The biochemical parameters like serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), serum bilirubin and total proteins were estimated to assess the liver function. Hepatic steatosis, centrilobular necrosis, and often swelling of the hepatic cytoplasm were observed in carbontetra chloride treated group, while these were completely absent in the extracts of T. lampas (300 mg/kg b.wt) treated groups (p < 0.01). The present investigation established pharmacological evidence to support the folkloric claim of hepatoprotective activity of T. lampas.Key words: Thespesia lampas, Carbon tetrachloride, Hepatoprotective, Rats, Root extracts  DOI = 10.3329/dujps.v7i1.1201Dhaka Univ. J. Pharm. Sci. 7(1): 11-13, 2008 (June)

scholarly journals Nutritional Status of Tuberculosis Patients in Against Serum Glutamate Pyruvate Transaminase Levels

2021 ◽  
Vol 1 (3) ◽  
pp. 14
Author(s):  
Iqbal Donarika Widagdo ◽  
Setyoko Setyoko ◽  
M. Riza Setiawan
Keyword(s):  
Nutritional Status ◽  
Rank Correlation ◽  
Test Results ◽  
Spearman Rank Correlation ◽  
Glutamate Pyruvate Transaminase ◽  
Tuberculosis Patients ◽  
Spearman Rank ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Background: Treatment of Tuberculosis patients are given in the form of a drugs combination. Some drugs which are Pyrazinamide, Isoniazid and Rifampicin can provide hepatotoxic side effects. Specific sign of hepatotoxic occurrence is increasing in the Serum Glutamate Pyruvate Transaminase levels. One of factors that influence hepatotoxicity is nutritional status. This study aims to determine the relationship between nutritional status and levels of Serum Glutamate Pyruvate Transaminase in tuberculosis patients.Methods: A non-experimental correlation study, retrospective approach with total sampling method, according to inclusion and exclusion criteria. Located at tCommunity Lung Health Center (BKPM) Ambarawa area. The analysis used the Spearman rank correlation testResults: Based on the test results of Spearman Rank correlation test, the correlation coefficient (r) is  -0.267. Hypothesis test results also showed 0.037 (p-value <0.05), which means the existence of a significant correlation between variabels.Conclusion: There is a relationship between nutritional status and levels of Serum Glutamate Pyruvate Transaminase in tuberculosis patients.

The Effectiveness of Tsu-San-Li (St-36) and Tai-Chung (Li-3) Acupoints for Treatment of Acute Liver Damage in Rats

2001 ◽  
Vol 29 (02) ◽  
pp. 221-226 ◽  
Author(s):  
Hsu-Jan Liu ◽  
Sheng-Feng Hsu ◽  
Chang-Chi Hsieh ◽  
Tin-Yun Ho ◽  
Ching-Liang Hsieh ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Acupuncture Therapy ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Male Rats ◽  
Acute Liver Damage ◽  
Glutamate Pyruvate Transaminase ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

This study investigated the effects of acupuncture on carbon tetrachloride (CCl4) induced acute liver injury in male rats (n = 36). The experimental groups were injected with CCl4 before, during, or after acupuncture therapy. Acupoints similar to the human Tsu-San-Li (St-36) and Tai-Chung (Li-3) were needled bilaterally. Rats treated with CCl4 had higher levels of serum glutamate-oxalate-transaminase (sGOT) and serum glutamate-pyruvate-transaminase (sGPT). Comparing the experimental groups, biochemical and pathological parameters of liver injury were significantly reduced when rats were acupunctured after, not before, CCl4-induced hepatotoxicity. Acupuncture at the Tsu-Sa-Li and Tai-Chung acupoints cannot prevent acute liver injury but may be effective in treating liver injury induced by carbon tetrachloride in rats.

Hepatoprotective Effect of Phyllanthus in Taiwan on Acute Liver Damage Induced by Carbon Tetrachloride

2006 ◽  
Vol 34 (03) ◽  
pp. 471-482 ◽  
Author(s):  
Chao-Ying Lee ◽  
Wen-Huang Peng ◽  
Hao-Yuan Cheng ◽  
Fei-Na Chen ◽  
Ming-Tsung Lai ◽  
...  
Keyword(s):  
Liver Damage ◽  
Reduced Glutathione ◽  
Protective Effects ◽  
Acute Liver Damage ◽  
Glutamate Pyruvate Transaminase ◽  
Methanolic Extracts ◽  
Antioxidant Agents ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

The effect of oral administration of Phyllanthus methanolic extracts (PME) (i.e. P. acidus, P. emblica, P. myrtifolius, P. multiflorus, P. amarus, P. debilis, P. embergeri, P. hookeri, P. tenellus, P. urinaria L.s. nudicarpus, P. urinaria L.s. urinaria) or gallic acid (GA) on the progression of acute liver damage induced by CCl 4 in rats was examined by morphological and biochemical methods. P. acidus, P. urinaria L.s. urinaria, GA at a dose of 0.5 g/kg, and P. emblica, P. urinaria L.s. nudicarpus at a dose of 1.0 g/kg attenuated CCl 4-induced increase in serum glutamate-oxalate-transaminase (GOT). P. acidus, P. urinaria L.s. nudicarpus, P. urinaria L.s. urinaria, GA at a dose of 0.5 g/kg, and P. emblica, P. amarus, P. hookeri, P. tenellus at a dose of 1.0 g/kg attenuated CCl 4-induced increase in serum glutamate-pyruvate-transaminase (GPT). Concurrently, P. acidus, P. multiflorus, P. embergeri, P. hookeri, P. tenellus and P. urinaria L.s. urinaria elevated the activity of liver reduced glutathione peroxidase (GSH-Px). Since the protective effects of P. acidus, P. emblica, P. myrtifolius, P. embergeri, P. urinaria L.s. nudicarpus, P. urinaria L.s. urinaria and GA correlate with a reduction in liver infiltration and focal necrosis observed using histological methods, these data demonstrate that P. acidus and P. urinaria L.s. urinaria are hepatoprotective and antioxidant agents.

scholarly journals Efek Proteksi Pemberian Ekstrak Daun Jamblang (Syzygium cumini) Terhadap Peningkatan Aktivitas Serum Glutamate Pyruvate Transaminase Tikus (Rattus norvegicus) Jantan Yang Diinduksi Timbal Asetat

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
ADILLA OKTARIZA ZARWIN
Keyword(s):  
Pyridoxal Phosphate ◽  
Glutamate Pyruvate Transaminase ◽  
Group Design ◽  
Post Test ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Post Hoc ◽  
Per Oral ◽  
Serum Glutamate ◽  
One Way Anova

Timbal (Pb) merupakan suatu logam berat yang bersifat toksik bagi tubuh. Timbal dapat menginduksi pembentukan radikal bebas yang dapat menimbulkan stres oksidatif sehingga berujung pada kematian sel. Kematian sel menstimulasi peningkatan enzim hati seperti SGPT dan SGOT. Tubuh memerlukan antioksidan eksogen untuk menghambat stres oksidatif akibat radikal bebas. Daun jamblang mengandung antioksidan alami seperti flavonoid dan fenolik yang dapat menangkal radikal bebas. Penelitian ini bertujuan untuk mengetahui efek proteksi pemberian ekstrak daun jamblang terhadap peningkatan aktivitas serum glutamate pyruvate transaminase (SGPT) tikus jantan yang diinduksi timbal asetat. Penelitian ini adalah penelitian eksperimental dengan rancangan randomized post test only group design menggunakan 15 ekor tikus jantan yang dibagi atas 3 kelompok yaitu kelompok kontrol negatif (K-) yang diberikan pakan standar, kelompok kontrol positif (K+) yang diberikan pakan standar dan timbal asetat dosis 40 mg/KgBB per oral, kelompok perlakuan 1 (P1) yang diberikan pakan standar serta timbal asetat dosis 40 mg/KgBB dan ekstrak daun jamblang dosis 150 mg/KgBB per oral. Penelitian ini dilakukan selama 28 hari dan di hari ke-29 tikus dimatikan lalu diambil serumnya untuk diperiksa kadar SGPT. Pemeriksaan kadar SGPT menggunakan tes berdasarkan kriteria IFCC tanpa pyridoxal phosphate dengan metode Sample Start. Data dianalisis dengan uji Shapiro Wilk, uji One Way Anova dan uji Post Hoc Tukey’s HSD. Hasil penelitian ini menunjukkan rerata kadar SGPT kelompok kontrol negatif (K-) 46,6 U/L, kelompok kontrol positif (K+) 73,9 U/L, dan kelompok perlakuan 1 (P1) 62,0 U/L. Hasil ini menunjukkan perbedaan bermakna rerata kadar SGPT semua kelompok hewan coba yang dibuktikan dengan nilai p < 0,05. Kesimpulan penelitian ini adalah adanya efek proteksi pemberian ekstrak daun jamblang terhadap peningkatan aktivitas SGPT tikus yang diiinduksi oleh timbal asetat. Kata Kunci: Timbal asetat, ekstrak daun jamblang, SGPT.

scholarly journals Pengaruh Hemolisis Terdapat Kadar Serum Glutamate Pyruvate Transaminase (SGPT) Sebagai Salah Satu Parameter Fungsi Hati

2017 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Hartono Kahar
Keyword(s):  
Liver Function ◽  
Clinical Laboratory ◽  
T Test ◽  
Glutamate Pyruvate Transaminase ◽  
Test Analysis ◽  
Key Word ◽  
Serum Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate ◽  
Paired T Test

 AbstractSGPT examination is one of the important checks on the state of a particular patient, for example, in patients with liver disease, hepatitis, and so forth. The other purpose is to detect the presence of liver damage. So there was an effect in the event of an increase in maupaun drop in levels. And the serum circumstances that do not qualify, such as serum hemolysis, will greatly affect the actual levels of ALT. Based on the research background, the authors formulate the problem as follows: "Is there any effect of hemolysis on SGPT levels as one of the parameters of liver function?". The purpose of this study is to prove whether there is an influence of hemolysis on SGPT levels as one of the parameters of liver function. The sample was 20 samples and 20 samples of hemolysis hemolysis. Location and time of the research conducted at Muhammadiyah Hospital Clinical Laboratory Lamongan ie in January and July. Of the 20 samples analyzed, showing that levels higher than the hemolysis ALT SGPT levels no hemolysis. When the serum through lysis will experience a significant increase. To test the effect of hemolysis on SGPT levels paired t test was used because the data from this study with normally distributed. The results of a paired t test analysis between serum hemolysis and no hemolysis on levels of serum ALT levels showed a significant value 0.00 thus P <0.05 it can be concluded that there is no difference between the levels of ALT SGPT levels of hemolysis with hemolysis. That means there is the influence of hemolysis on SGPT levels as one of the parameters of liver function. Key word : hemolysis, glutamate pyruvate transminase 

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