Interaction of pentoxifylline with human erythrocytes. I. Interaction of xanthine derivatives with human erythrocyte ghosts.

In this paper we report the lead (Pb) and calcium (Ca) uptake by erythrocyte ghosts. In both cases the transport was carried out by a passive transport system with two kinetic components (Michaelis-Menten and Hill). Pb and Ca were capable of inhibiting the transport of the other metal in a non-competitive way. Under hyperpolarization, the uptakes of Ca and Pb were enhanced and the Michaelis-Menten component prevailed. Both Ca and Pb uptakes were inhibited by N-ethyl-maleimide to the same extent. These results indicate that Pb and Ca share the same permeability pathway in human erythrocytes and that this transport system is electrogenic.

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Shape transformation of erythrocyte ghosts depends on ion concentrations

Bioscience Reports ◽

10.1007/bf01116559 ◽

1985 ◽

Vol 5 (5) ◽

pp. 417-423 ◽

Cited By ~ 13

Author(s):

Andreas Herrmann ◽

Peter Müller ◽

Roland Glaser

Keyword(s):

Human Erythrocyte ◽

Transmembrane Potential ◽

Human Erythrocytes ◽

Erythrocyte Ghosts ◽

Shape Transformation ◽

Ion Concentrations ◽

Nacl Concentration ◽

Shape Transformations

Resealed human erythrocyte ghosts undergo shape transformations similar to those of intact erythrocytes. The results indicate that the shape of these ghosts depends on the inner as well as on the outer NaCl concentration. A correlation between shape and calculated transmembrane potential was established similar to that for intact human erythrocytes.

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FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS

The Journal of Cell Biology ◽

10.1083/jcb.63.1.1 ◽

1974 ◽

Vol 63 (1) ◽

pp. 1-11 ◽

Cited By ~ 103

Author(s):

Hava Peretz ◽

Zivia Toister ◽

Yehudith Laster ◽

Abraham Loyter

Keyword(s):

Red Blood Cells ◽

Clostridium Perfringens ◽

Human Erythrocyte ◽

Blood Cells ◽

Sendai Virus ◽

Iodoacetic Acid ◽

Human Erythrocytes ◽

Erythrocyte Ghosts ◽

Human Red Blood Cells ◽

Bivalent Cations

Sendai virus is able to induce the fusion of human erythrocytes. Bivalent cations or ATP are not essential for polyerythrocyte formation. High fusion indices were obtained when Sendai virus was added to cells incubated in the presence of both EDTA and iodoacetic acid. Human erythrocyte ghosts prepared by gradual hemolysis still retain the potential to undergo virus-induced fusion. Fusion of human red blood cells without the addition of viruses was obtained by incubation of erythrocytes at pH 10.5 in the presence of Ca++ (40 mM) or by addition of phospholipase C Clostridium perfringens preparations to cells previously agglutinated or polylysine.

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ATP Dependent Uptake of Zinc by Human Erythrocyte Ghosts

Zeitschrift für Naturforschung C ◽

10.1515/znc-1978-3-407 ◽

1978 ◽

Vol 33 (3-4) ◽

pp. 210-215 ◽

Cited By ~ 4

Author(s):

Georg Schmetterer

Keyword(s):

Atomic Absorption ◽

Energy Dependence ◽

Absorption Spectroscopy ◽

Atomic Absorption Spectroscopy ◽

Human Erythrocyte ◽

Human Erythrocytes ◽

Erythrocyte Ghosts ◽

Passive Mechanism ◽

Resealed Ghosts

The energy dependence of Zn2+ accumulation by human erythrocytes was studied by preparing resealed ghosts loaded with ATP or AMP. Uptake of Zn2+ was followed by radioactivity or atomic absorption spectroscopy measurements. Zn2+ taken up is not adsorbed by the membrane but transported into the ghosts. Ghosts containing ATP took up more Zn2+ than those containing AMP. The effect is not specific for ATP, however, GTP and CTP showing the same increased uptake. Zn2+ uptake by ATP ghosts can be further drastically enhanced by addition of Cu2t. Therefore, in spite of the enhancement of Zn2+ uptake by ATP, Zn2+ seems to be accumulated by a passive mechanism even when the ghosts contain ATP. The accumulation is probably brought about by binding of Zn2+ to substances inside the ghosts.

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Elemental analysis of human erythrocytes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010015318x ◽

1989 ◽

Vol 47 ◽

pp. 242-243

Author(s):

S. A. Livesey ◽

A. A. del Campo ◽

E. S. Griffey ◽

D. Ohlmer ◽

T. Schifani ◽

...

Keyword(s):

Sample Preparation ◽

Elemental Analysis ◽

Human Erythrocyte ◽

Spectroscopic Analysis ◽

Model System ◽

Human Erythrocytes ◽

List Type ◽

Chemical Fixation ◽

Ethanol Dehydration ◽

Lowicryl K4m

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.

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