Induction of Colony Formation of Microcystis aeruginosa by Controlling Extracellular Polysaccharides and Metal Cation Concentrations

2018 ◽  
Vol 51 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Ken Omori ◽  
Masahiro Sato ◽  
Yoshimasa Amano ◽  
Motoi Machida
Keyword(s):  
Microcystis Aeruginosa ◽  
Metal Cation ◽  
Colony Formation ◽  
Extracellular Polysaccharides

Effects of lead(II) on the extracellular polysaccharide (EPS) production and colony formation of cultured Microcystis aeruginosa

2013 ◽  
Vol 67 (4) ◽  
pp. 803-809 ◽  
Author(s):  
Xiang-dong Bi ◽  
Shu-lin Zhang ◽  
Wei Dai ◽  
Ke-zhing Xing ◽  
Fan Yang
Keyword(s):  
Heavy Metals ◽  
Microcystis Aeruginosa ◽  
Colony Formation ◽  
Extracellular Polysaccharide ◽  
Extracellular Polysaccharides ◽  
Control Group ◽  
Natural Conditions ◽  
Laboratory Conditions ◽  
Large Colony ◽  
Middle Colonies

To investigate the effects of lead(II) on the production of extracellular polysaccharides (EPS), including bound extracellular polysaccharides (bEPS) and soluble extracellular polysaccharides (sEPS), and the colony formation of Microcystis aeruginosa, cultures of M. aeruginosa were exposed to four concentrations (5.0, 10.0, 20.0 and 40.0 mg/L) of lead(II) for 10 d under controlled laboratory conditions. The results showed that 5.0 and 10.0 mg/L lead(II) stimulated M. aeruginosa growth throughout the experiment while 20.0 and 40.0 mg/L lead(II) inhibited M. aeruginosa growth in the first 2 d exposure and then stimulated it. As compared to the control group, significant increases in the bEPS and sEPS production were observed in 20.0 and 40.0 mg/L lead(II) treatments (P < 0.05). Large colony formations were not observed throughout the experiment. However, four tested concentrations of lead(II) could significantly promote the formation of small and middle colonies after 10 d exposure (P < 0.05), and 40.0 mg/L lead(II) had the best stimulatory effect. Lead(II) could stimulate bEPS production, which conversely promoted colony formation, suggesting that heavy metals might be contributing to the bloom-forming of M. aeruginosa in natural conditions.

Impacts of different extracellular polysaccharides on colony formation and buoyancy of Microcystis aeruginosa

2020 ◽  
Vol 56 ◽  
pp. 28
Author(s):  
Kai Wei ◽  
Yoshimasa Amano ◽  
Motoi Machida
Keyword(s):  
Microcystis Aeruginosa ◽  
Colony Size ◽  
Colony Formation ◽  
Culture Medium ◽  
Divalent Metal ◽  
Extracellular Polysaccharides ◽  
Size Analysis ◽  
Light Conditions ◽  
Divalent Metal Cations ◽  
Calcium And Magnesium

On the surface of Microcystis cells, there is a carbohydrate called extracellular polysaccharides (EPS) playing a significant role in the colony formation of Microcystis. EPS consists of tightly cell-bound EPS (TB-EPS), and both of these substances are considered to be strongly related to the colony formation and buoyancy of Microcystis. In this study, Microcystis aeruginosa (strain: NIES-843) was used to examine the effects of EPS, TB-EPS, and divalent metal cations such as calcium and magnesium on the buoyancy and colony formation of M. aeruginosa NIES-843. Under various light conditions, the addition of TB-EPS into the culture medium induced M. aeruginosa NIES-843 to obtain high buoyancy at concentrations of Ca2+ and Mg2+ concentrations of 10 mg/L and 30 mg/L, respectively. Under the absence of light, the addition of EPS could lead M. aeruginosa to form a colony and obtain buoyancy, and the addition of TB-EPS could not significantly change the buoyancy of M. aeruginosa NIES-843. The colony size analysis showed that at the same cationic concentration, the addition of TB-EPS could induce M. aeruginosa to form the largest colony and present strong buoyancy. This study suggested that temperature and illumination are conducive to colony formation and present higher buoyancy of M. aeruginosa.

The extract of aquatic macrophyte Carex cinerascens induced colony formation in bloom-forming cyanobacterium Microcystis aeruginosa

2020 ◽  
Vol 27 (34) ◽  
pp. 42276-42282 ◽  
Author(s):  
Lin Li ◽  
Xiao-ling Jing ◽  
Ling Wang ◽  
Zhi-gao Zeng ◽  
Wen-hong Chen ◽  
...  
Keyword(s):  
Microcystis Aeruginosa ◽  
Colony Formation ◽  
Aquatic Macrophyte

Physiological response of Microcystis aeruginosa to the extracellular substances from an Aeromonas sp.

RSC Advances ◽  
2016 ◽  
Vol 6 (105) ◽  
pp. 103662-103667 ◽  
Author(s):  
Xing Zhang ◽  
Tao Song ◽  
Hao Ma ◽  
Li Li
Keyword(s):  
Enzyme Activity ◽  
Microcystis Aeruginosa ◽  
Physiological Response ◽  
Physiological Responses ◽  
Extracellular Polysaccharides ◽  
Malondialdehyde Content ◽  
Extracellular Substances

Physiological responses of M. aeruginosa to different amounts of the extracellular substances from Aeromonas sp. were studied, including the changes of extracellular polysaccharides content, enzyme activity and malondialdehyde content.

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