Study on the experimental ulcerative colitis model induced by dextran sulfate sodium in rats: Estimation of mucosal erosions by the alcian blue-staining method.

ContextInflammatory bowel disease, including ulcerative colitis and Crohn’s disease, comprising a broad spectrum of diseases those have in common chronic inflammation of the gastrointestinal tract, histological alterations and an increased activity levels of certain enzymes, such as, metalloproteinases.ObjectivesEvaluate a possible correlation of disease activity index with the severity of colonic mucosal damage and increased activity of metalloproteinases in a model of ulcerative colitis induced by dextran sulfate sodium.MethodsColitis was induced by oral administration of 5% dextran sulfate sodium for seven days in this group (n=10), whereas control group (n=16) received water. Effects were analyzed daily by disease activity index. In the seventh day, animals were euthanized and hematological measurements, histological changes (hematoxylin and eosin and Alcian Blue staining), myeloperoxidase and metalloproteinase activities (MMP-2 and MMP-9) were determined.ResultsDextran sulfate sodium group showed elevated disease activity index and reduced hematological parameters. Induction of colitis caused tissue injury with loss of mucin and increased myeloperoxidase (P<0.001) and MMP-9 activities (45 fold) compared to the control group.ConclusionsIn this study, we observed a disease activity index correlation with the degree of histopathological changes after induction of colitis, and this result may be related mainly to the increased activity of MMP-9 and mieloperoxidase.

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Effects of Lactobacillus gasseri SBT2055 on Dextran Sulfate Sodium-Induced Ulcerative Colitis Model in Rats

Bioscience and Microflora ◽

10.12938/bifidus1996.21.179 ◽

2002 ◽

Vol 21 (3) ◽

pp. 179-183 ◽

Cited By ~ 5

Author(s):

Eiichi IMAI ◽

Kenji FUKUI ◽

Noriyasu OHTA ◽

Toshie TOMITSUKA ◽

Yasuyuki SETO ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Lactobacillus Gasseri ◽

Colitis Model

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Cryptotanshinone protects dextran sulfate sodium‐induced experimental ulcerative colitis in mice by inhibiting intestinal inflammation

Phytotherapy Research ◽

10.1002/ptr.6693 ◽

2020 ◽

Vol 34 (10) ◽

pp. 2639-2648

Author(s):

Xiangjing Min ◽

Xi Zeng ◽

Wenwen Zhao ◽

Zhiwu Han ◽

Ying Wang ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Intestinal Inflammation ◽

Experimental Ulcerative Colitis

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The ameliorative effect of a Lactobacillus strain with good adhesion ability against dextran sulfate sodium-induced murine colitis

Food & Function ◽

10.1039/c8fo01453a ◽

2019 ◽

Vol 10 (1) ◽

pp. 397-409 ◽

Cited By ~ 9

Author(s):

Guangqiang Wang ◽

Yingnan Liu ◽

Zhi Lu ◽

Yiting Yang ◽

Yongjun Xia ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Good Adhesion ◽

Disease Symptoms ◽

Murine Colitis ◽

Adhesion Ability ◽

Ameliorative Effect ◽

Excellent Adhesion ◽

Colitis Model

The objective of this study was to effectively screen out a Lactobacillus strain with excellent adhesion ability and ameliorative effect on the disease symptoms of a murine ulcerative colitis model.

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Oral administration of Lentinus edodes β-glucans ameliorates DSS-induced ulcerative colitis in mice via MAPK-Elk-1 and MAPK-PPARγ pathways

Food & Function ◽

10.1039/c6fo01043a ◽

2016 ◽

Vol 7 (11) ◽

pp. 4614-4627 ◽

Cited By ~ 22

Author(s):

Limin Shi ◽

Qinlu Lin ◽

Tao Yang ◽

Ying Nie ◽

Xinhua Li ◽

...

Keyword(s):

Ulcerative Colitis ◽

Oral Administration ◽

Molecular Mechanism ◽

Sodium Salt ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Lentinus Edodes ◽

Raw264.7 Cell ◽

Colitis Model ◽

Inflammatory Effect

To evaluate the anti-inflammatory effect of β-glucans fromLentinus edodes, and its molecular mechanism, the dextran sulfate sodium salt (DSS) induced colitis model of mice and the LPS-stimulated RAW264.7 cell inflammation model were used in this study.

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Beneficial and preventive effect of chitin nanofibrils in a dextran sulfate sodium-induced acute ulcerative colitis model

Carbohydrate Polymers ◽

10.1016/j.carbpol.2011.09.036 ◽

2012 ◽

Vol 87 (2) ◽

pp. 1399-1403 ◽

Cited By ~ 41

Author(s):

Kazuo Azuma ◽

Tomohiro Osaki ◽

Takashi Wakuda ◽

Shinsuke Ifuku ◽

Hiroyuki Saimoto ◽

...

Keyword(s):

Ulcerative Colitis ◽

Dextran Sulfate ◽

Dextran Sulfate Sodium ◽

Preventive Effect ◽

Colitis Model

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Basophil counting with a new staining method using alcian blue

Blood ◽

10.1182/blood.v46.2.279.bloodjournal462279 ◽

1975 ◽

Vol 46 (2) ◽

pp. 279-286 ◽

Cited By ~ 1

Author(s):

HS Gilbert ◽

L Ornstein

Keyword(s):

Alcian Blue ◽

Toluidine Blue ◽

Staining Method ◽

Blue Staining ◽

Total Leukocyte Count ◽

Blue Dye ◽

Alcian Blue Staining ◽

Nuclear Staining ◽

Chamber Method ◽

Cytoplasmic Proteins

Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspecific nuclear staining. This objective is achieved because of the differences in stability of alcian blue-heparin, alcian blue-nucleic acid, lanthanum-heparin, and lanthanum-mucliec acid complexes. Reduction of pH after staining also favors solubilization of leukocyte cytoplasmic proteins, providing greater contrast between stained and unstained cells by reducing light scattering of the unstained leukocytes . The alcian blue staining method is suitable both for chamber basophil counting and automated basophil counting using continuous-flow sampling and electro-optical detection. The new staining method was evaluated by comparing it with the chamber counting method using toluidine blue in a triple-blind study in which the results of basophil counting by the alcian blue chamber method, alcian blue automated instrument method, and the toluidine blue chamber method were analyzed for reproducibility and compared with an indirect basophil count obtained from a 1000-cell leukocyte differential and a total leukocyte count. Both alcian blue staining methods gave greater reporducibility that toluidine blue and were more accurate, as evidinced by a significantly higher correlation with the indirect basophil count. The improved reproducibility, accuracy, and convenience of this method over existing methods should facilitate the collection of more meaningful information about circulating basophil levels in health and disease.

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Periodic acid-Schiff and alcian blue immunohistochemistry to detect mucin in mucinous breast carcinoma

Medical Journal of Indonesia ◽

10.13181/mji.oa.192768 ◽

2020 ◽

Vol 29 (1) ◽

pp. 53-7 ◽

Cited By ~ 1

Author(s):

Primariadewi Rustamadji ◽

Jason Wibowo ◽

Belinda Murtani ◽

Christy Magdalena

Keyword(s):

Breast Carcinoma ◽

Alcian Blue ◽

Staining Method ◽

Periodic Acid ◽

Mucinous Carcinoma ◽

Blue Staining ◽

Alcian Blue Staining ◽

Periodic Acid Schiff ◽

Pas Staining ◽

Mucinous Breast Carcinoma

BACKGROUND Detection of mucins has been shown to correlate with several clinicopathological characteristics in patients. Currently, periodic acid-Schiff (PAS) and alcian blue staining methods are the histochemistry staining techniques that are frequently used to detect mucin. This study was aimed to evaluate PAS and alcian blue staining in differentiating mucin characteristics between invasive carcinoma of no special type (ICNST) with mucinous degeneration and mucinous carcinoma. METHODS This cross-sectional study of 33 cases included biopsies of mucinous breast carcinoma and ICNST with mucin degeneration that were histologically verified using hematoxylin and eosin (H&E) staining. The PAS and alcian blue staining were conducted in the Laboratory of Histochemistry, Department of Anatomical Pathology, Cipto Mangunkusumo Hospital. Data were recorded using SPSS software, version 21 (IBM Corp, USA). RESULTS There were 17 cases of ICNST with mucinous degeneration and 16 cases of mucinous carcinoma with age varied from 27 to 75 years. PAS had sensitivity of 87.5% and specificity of 41.2%. Alcian blue had sensitivity of 43.8% and specificity of 82.4%. CONCLUSIONS PAS staining method is better than the alcian blue staining method in distinguishing between ICNST with mucinous degeneration and mucinous carcinoma. In the limited setting laboratory, PAS staining alone can be considered to detect mucin.

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